LOCUS       sequence-200238-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a circularly permuted mCerulean3 for FRET sensor optimization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Markwardt ML, Seckinger KM, Rizzo MA
  TITLE     Regulation of Glucokinase by Intracellular Calcium Levels in 
            Pancreatic beta Cells.
  JOURNAL   J Biol Chem. 2016 Feb 5;291(6):3000-9. doi: 10.1074/jbc.M115.692160.
            Epub 2015 Dec 23.
  PUBMED    26698632
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.692160"; journalName: "J Biol Chem"; date: 
            "2016-02-5- 5"; volume: "291"; issue: "6"; pages: "3000-9"
COMMENT     Sequence Label: cp173-mCer3-C1
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     137..158
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(261..282)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(522..541)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
       61 ccgtgctgct gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca
      121 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
      181 gcatggacga gctgtacaag ggtggcagcg gtggcatggt gagcaagggc gaggagctgt
      241 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      301 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      361 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg agctggggcg
      421 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      481 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      541 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      601 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatccacg
      661 gcaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcggcctca
      721 actgcaacat cgagtccgga ctca
//
