LOCUS       sequence-200244-        1258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cyan NMMII phosphorylation sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Rizzo
  TITLE     Rizzo lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mCer3-myosinRLC
FEATURES             Location/Qualifiers
     source          1..1258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgagctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaacgccat ccacggcaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg ccaacttcgg cctcaactgc aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc
      721 ggactcagat ctcgagttat gtccagcaag cgggccaaag ccaagaccac caagaagcgg
      781 ccacagcggg ccacatccaa tgtcttcgca atgtttgacc agtcccagat ccaggagttt
      841 aaggaggctt tcaacatgat tgaccagaac cgtgatggct tcattgacaa ggaggacctg
      901 cacgacatgc tggcctcgct ggggaagaac cccacagacg aatacctgga gggcatgatg
      961 agcgaggccc cggggcccat caacttcacc atgttcctca ccatgtttgg ggagaagctg
     1021 aacggcacgg accccgagga tgtgattcgc aacgcctttg cctgcttcga cgaggaagcc
     1081 tcaggtttca tccatgagga ccacctccgg gagctgctca ccaccatggg tgaccgcttc
     1141 acagatgagg aagtggacga gatgtaccgg gaggcaccca ttgataagaa aggcaacttc
     1201 aactacgtgg agttcacccg catcctcaaa catggcgcca aggataaaga cgactagg
//
