LOCUS       sequence-200246-        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red NMMII phosphorylation sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Rizzo
  TITLE     Rizzo lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: mCherry-myosinRLC
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     414..433
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
      421 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagtc cggactcaga
      721 tctcgagtta tgtccagcaa gcgggccaaa gccaagacca ccaagaagcg gccacagcgg
      781 gccacatcca atgtcttcgc aatgtttgac cagtcccaga tccaggagtt taaggaggct
      841 ttcaacatga ttgaccagaa ccgtgatggc ttcattgaca aggaggacct gcacgacatg
      901 ctggcctcgc tggggaagaa ccccacagac gaatacctgg agggcatgat gagcgaggcc
      961 ccggggccca tcaacttcac catgttcctc accatgtttg gggagaagct gaacggcacg
     1021 gaccccgagg atgtgattcg caacgccttt gcctgcttcg acgaggaagc ctcaggtttc
     1081 atccatgagg accacctccg ggagctgctc accaccatgg gtgaccgctt cacagatgag
     1141 gaagtggacg agatgtaccg ggaggcaccc attgataaga aaggcaactt caactacgtg
     1201 gagttcaccc gcatcctcaa acatggcgcc aaggataaag acgactag
//
