LOCUS       sequence-200301-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding mTurquoise.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-mTurquoise
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             176..892
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISDNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(206..234)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     829..849
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      930..1016
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1554..2504)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2552..2774
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3266..3360)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3391..4185)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3502..3521)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4112..4131
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4364..4382
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttcaccacag ctaacaccac gtcgtcccta tctgctgccc taggtctatg agtggttgct
       61 ggataacttt acgggcatgc ataaggctcg tatgatatat tcagggagac cacaacggtt
      121 tccctctaca aataattttg tttaactttt ctagatttaa gaaggagata tacatatgag
      181 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      241 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      301 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      361 tactctctct tggggtgttc aatgctttgc aagataccca gatcatatga aacagcatga
      421 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
      481 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      541 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
      601 atacaactac atctcagaca atgtatacat cacggcagac aaacaaaaga atggaatcaa
      661 agctaacttc aaaattagac acaacattga agatggaggc gttcaactag cagaccatta
      721 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
      781 cacacaatct aagctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
      841 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aaatgtccag
      901 acctgcaggc atgcaagctc tagaggcatc aaataaaacg aaaggctcag tcgaaagact
      961 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1021 cgccctagac ctagggtacg ggttttgctg cccgcaaacg ggctgttctg gtgttgctag
     1081 tttgttatca gaatcgcaga tccggcttca gccggtttgc cggctgaaag cgctatttct
     1141 tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca
     1201 gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg
     1261 taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac
     1321 ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa
     1381 cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
     1441 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac
     1501 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
     1561 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
     1621 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg
     1681 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg
     1741 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
     1801 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
     1861 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
     1921 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
     1981 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
     2041 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
     2101 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
     2161 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     2221 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     2281 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
     2341 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     2401 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     2461 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     2521 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt
     2581 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     2641 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     2701 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     2761 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     2821 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     2881 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     2941 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     3001 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     3061 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     3121 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     3181 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     3241 ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     3301 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     3361 caagcgagct ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa
     3421 ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca
     3481 ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc
     3541 cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat
     3601 attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc
     3661 cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc
     3721 ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg
     3781 gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat
     3841 gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc
     3901 gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg
     3961 aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc
     4021 accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac
     4081 ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac
     4141 ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     4201 tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     4261 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     4321 cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     4381 gccctttcgt c
//
