LOCUS       sequence-200308-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding moxCerulean3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-moxCerulean3
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..63
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(278..306)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     901..921
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1002..1088
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1626..2576)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2624..2846
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3338..3432)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3463..4257)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3574..3593)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4184..4203
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
       61 ggccctttcg tcttcaccac agctaacacc acgtcgtccc tatctgctgc cctaggtcta
      121 tgagtggttg ctggataact ttacgggcat gcataaggct cgtatgatat attcagggag
      181 accacaacgg tttccctcta caaataattt tgtttaactt ttctagattt aagaaggaga
      241 tatacatatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      301 agatggtgat gttaatgggc acaaattttc tgtccgtgga gagggtgaag gtgatgcaac
      361 aaacggaaaa cttaccctta aatttatttc cactactgga aaactacctg ttccatggcc
      421 aacacttgtc actactttaa gctggggtgt tcaatcgttt gcaagatacc cagatcatat
      481 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      541 atttttcaaa gatgacggga cctacaagac acgtgctgaa gtcaagtttg aaggtgatac
      601 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      661 acacaaattg gaatacaacg ctatccacgg caatgtatac atcacggcag acaaacaaaa
      721 gaatggaatc aaagctaact tcggcctcaa ctgcaacgtt gaagatggaa gcgttcaact
      781 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      841 ccattacctg tccacacaat ctaagctttc gaaagatccc aacgaaaaga gagaccacat
      901 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
      961 ataaatgtcc agacctgcag gcatgcaagc tctagaggca tcaaataaaa cgaaaggctc
     1021 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     1081 ggacaaatcc gccgccctag acctagggta cgggttttgc tgcccgcaaa cgggctgttc
     1141 tggtgttgct agtttgttat cagaatcgca gatccggctt cagccggttt gccggctgaa
     1201 agcgctattt cttccagaat tgccatgatt ttttccccac gggaggcgtc actggctccc
     1261 gtgttgtcgg cagctttgat tcgataagca gcatcgcctg tttcaggctg tctatgtgtg
     1321 actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt tgctttgttt
     1381 tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca ttgtcgatct
     1441 gttcatggtg aacagctttg aatgcaccaa aaactcgtaa aagctctgat gtatctatct
     1501 tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatg taacggtgaa
     1561 cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca agagccataa
     1621 gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc
     1681 gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt
     1741 gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt
     1801 ccgttatgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt
     1861 atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa
     1921 atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg
     1981 tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag
     2041 ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg
     2101 tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg
     2161 ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga
     2221 taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag
     2281 tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc
     2341 gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct actgatgttc
     2401 atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt
     2461 tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt
     2521 aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat
     2581 ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa
     2641 ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga
     2701 aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt
     2761 gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga
     2821 atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg
     2881 atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt
     2941 agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga ccatcaggca
     3001 cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc tggcagtgaa
     3061 tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac tacccataat
     3121 acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc
     3181 tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct gaccacttcg
     3241 gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca gcggtatcat
     3301 caacaggctt acccgtctta ctgtccctag tgcttggatt ctcaccaata aaaaacgccc
     3361 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     3421 tcaacaggag tccaagcgag ctctcgaacc ccagagtccc gctcagaaga actcgtcaag
     3481 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     3541 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     3601 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     3661 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     3721 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     3781 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     3841 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     3901 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     3961 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     4021 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag
     4081 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     4141 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     4201 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg
     4261 aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg
     4321 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     4381 agctggcaat t
//
