LOCUS       sequence-20033-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Hallows WC, Lee S, Denu JM
  TITLE     Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5.
  PUBMED    16790548
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 5. 103(27):10230-5."
COMMENT     Sequence Label: SIRT1.1
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..30
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(54..68,69..74,75..91,92..98)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    74..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    106..122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             141..152
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             171..188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa
       61 aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac
      121 aatttcacac agaattcatt aaagaggaga aattaactat gagaggatcg catcaccatc
      181 accatcacgg atccatggcg gacgaggcgg cggcggcagg cggggagcaa gaggcccagg
      241 cgactgcggc ggctggggaa ggagacaatg ggccgggcct gcagggccca tctcgggagc
      301 caccgctggc cgacaacttg tacgacgaag acgacgacga cgagggcgag gaggaggaag
      361 aggcggcggc ggcggcgatt gggtaccgag ataaccttct gttcggtgat gaaattatca
      421 ctaatggttt tcattcctgt gaaagtgatg aggaggatag agcctcacat gcaagctcta
      481 gtgactggac tccaaggcca cggataggtc catatacttt tgttcagcaa catcttatga
      541 ttggcacaga tcctcgaaca attcttaaag atttattgcc ggaaacaata cctccacctg
      601 agttggatga tatgacactg tggcagattg ttattaatat cctttcagaa ccaccaaaaa
      661 ggaaaaaaag aaaagatatt aatacaattg aagatgctgt gaaattac
//
