LOCUS       sequence-20034-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Hallows WC, Lee S, Denu JM
  TITLE     Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5.
  PUBMED    16790548
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 5. 103(27):10230-5."
COMMENT     Sequence Label: SIRT3L-12
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(37..51,52..57,58..74,75..81)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    57..73
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    89..105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             124..135
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cgtatcacga ggccctttcg tcttcacctc gagaaatcat aaaaaattta tttgctttgt
       61 gagcggataa caattataat agattcaatt gtgagcggat aacaatttca cacagaattc
      121 attaaagagg agaaattaac tatgagagga tcgcatcacc atcaccatca cggatccaag
      181 gggaagcttt ccctgcagga tgtagctgag ctgattcggg ccagagcctg ccagagggtg
      241 gtggtcatgg tgggggccgg catcagcaca cccagtggca ttccagactt cagatcgccg
      301 gggagtggcc tgtacagcaa cctccagcag tacgatctcc cgtaccccga ggccattttt
      361 gaactcccat tcttctttca caaccccaag ccctttttca ctttggccaa ggagctgtac
      421 cctggaaact acaagcccaa cgtcactcac tactttctcc ggctgcttca tgacaagggg
      481 ctgcttctgc ggctctacac gcagaacatc gatgggcttg agagagtgtc gggcatccct
      541 gcctcaaagc tggttgaagc tcatggaacc tttgcctctg ccacctgcac agtctgccaa
      601 agacccttcc caggggagga cattcgggct gacgtgatgg cagacagggt tccccgctgc
      661 ccggtctgca ccggcgttgt gaagcccgac attgtgttct ttggggagcc gctgccccag
      721 aggttcttgc tgcatg
//
