LOCUS       sequence-200375-       15200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  polycistronic vector for expression of E. coli core RNAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15200)
  AUTHORS   Svetlov V, Artsimovitch I
  TITLE     Purification of bacterial RNA polymerase: tools and protocols.
  JOURNAL   Methods Mol Biol. 2015;1276:13-29. doi: 10.1007/978-1-4939-2392-2_2.
  PUBMED    25665556
REFERENCE   2  (bases 1 to 15200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2015"; volume: "1276:13-29. doi"; pages: " 
            10.1007/978-1-4939-2392-2_2"
COMMENT     Sequence Label: PVS10
FEATURES             Location/Qualifiers
     source          1..15200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        493..597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(598..666,667..1458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(816..835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1629..2217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2118..2137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2371..2388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2403..2542
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2468..2487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2628..2650
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2644..2835)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3644..4726)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4688..4707
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4727..4804)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5010..5029
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5113..5132
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5113..5131
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5132..5156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5171..5193
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             14616..14633
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             14753..15028
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     CDS             15044..15061
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(15114..15132)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      15128..15175
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1141 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     1321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     1801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2401 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca
     2461 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     2521 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     2581 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     2701 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     2761 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     2821 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     2881 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     2941 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
     3001 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
     3061 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     3121 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     3181 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     3241 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
     3301 tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
     3361 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
     3421 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
     3481 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     3541 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
     3601 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
     3661 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3721 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     3781 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     3841 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
     3901 tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
     3961 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     4021 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     4081 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     4141 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     4201 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     4261 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     4321 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     4381 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     4441 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     4501 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     4561 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     4621 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     4681 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     4741 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     4801 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     4861 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
     4921 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     4981 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     5041 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
     5101 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
     5161 tagaaataat tttgtttaac tttaagaagg agatatacat atgcagggtt ctgtgacaga
     5221 gtttctaaaa ccgcgcctgg ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac
     5281 ccttgagcct ttagagcgtg gctttggcca tactctgggt aacgcactgc gccgtattct
     5341 gctctcatcg atgccgggtt gcgcggtgac cgaggttgag attgatggtg tactacatga
     5401 gtacagcaca aaagaaggcg ttcaggaaga tatcctggaa atcctgctca acctgaaagg
     5461 gctggcggtg agagttcagg gcaaagatga agttattctt accttgaata aatctggcat
     5521 tggccctgtg actgcagccg atatcaccca cgacggtgat gtcgaaatcg tcaagccgca
     5581 gcacgtgatc tgccacctga ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca
     5641 gcgcggtcgt ggttatgtgc cggcttctac ccgaattcat tcggaagaag atgagcgccc
     5701 aatcggccgt ctgctggtcg acgcatgcta cagccctgtg gagcgtattg cctacaatgt
     5761 tgaagcagcg cgtgtagaac agcgtaccga cctggacaag ctggtcatcg aaatggaaac
     5821 caacggcaca atcgatcctg aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca
     5881 actggaggct ttcgttgact tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc
     5941 agagttcgat ccgatcctgc tgcgccctgt tgacgatctg gaattgactg tccgctctgc
     6001 taactgcctt aaagcagaag ctatccacta tatcggtgat ctggtacagc gtaccgaggt
     6061 tgagctcctt aaaacgccta accttggtaa aaaatctctt actgagatta aagacgtgct
     6121 ggcttcccgt ggactgtctc tgggcatgcg cctggaaaac tggccaccgg caagcatcgc
     6181 tgacgagtaa ccggatcccc gatccgtgga cttgtcagcg actgaggaaa cagaccatgg
     6241 atccccgatc cgtcgacttg tcagcgagct gaggaaccct atggtttact cctataccga
     6301 gaaaaaacgt attcgtaagg attttggtaa acgtccacaa gttctggatg taccttatct
     6361 cctttctatc cagcttgact cgtttcagaa atttatcgag caagatcctg aagggcagta
     6421 tggtctggaa gctgctttcc gttccgtatt cccgattcag agctacagcg gtaattccga
     6481 gctgcaatac gtcagctacc gccttggcga accggtgttt gacgtccagg aatgtcaaat
     6541 ccgtggcgtg acctattccg caccgctgcg cgttaaactg cgtctggtga tctatgagcg
     6601 cgaagcgccg gaaggcaccg taaaagacat taaagaacaa gaagtctaca tgggcgaaat
     6661 tccgctcatg acagacaacg gtacctttgt tatcaacggt actgagcgtg ttatcgtttc
     6721 ccagctgcac cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa cccactcttc
     6781 gggtaaagtg ctgtataacg cgcgtatcat cccttaccgt ggttcctggc tggacttcga
     6841 attcgatccg aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac tgcctgcgac
     6901 catcattctg cgcgccctga actacaccac agagcagatc ctcgacctgt tctttgaaaa
     6961 agttatcttt gaaatccgtg ataacaagct gcagatggaa ctggtgccgg aacgcctgcg
     7021 tggtgaaacc gcatcttttg acatcgaagc taacggtaaa gtgtacgtag aaaaaggccg
     7081 ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac tgatcgaagt
     7141 cccggttgag tacatcgcag gtaaagtggt tgctaaagac tatattgatg agtctaccgg
     7201 cgagctgatc tgcgcagcga acatggagct gagcctggat ctgctggcta agctgagcca
     7261 gtctggtcac aagcgtatcg aaacgctgtt caccaacgat ctggatcacg gcccatatat
     7321 ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg tagaaatcta
     7381 ccgcatgatg cgccctggcg agccgccgac tcgtgaagca gctgaaagcc tgttcgagaa
     7441 cctgttcttc tccgaagacc gttatgactt gtctgcggtt ggtcgtatga agttcaaccg
     7501 ttctctgctg cgcgaagaaa tcgaaggttc cggtatcctg agcaaagacg acatcattga
     7561 tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg atatcgacca
     7621 cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt tccgcgttgg
     7681 cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc tggataccct
     7741 gatgccacag gatatgatca acgccaagcc gatttccgca gcagtgaaag agttcttcgg
     7801 ttccagccag ctgtctcagt ttatggacca gaacaacccg ctgtctgaga ttacgcacaa
     7861 acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag gcttcgaagt
     7921 tcgagacgta cacccgactc actacggtcg cgtatgtcca atcgaaaccc ctgaaggtcc
     7981 gaacatcggt ctgatcaact ctctgtccgt gtacgcacag actaacgaat acggcttcct
     8041 tgagactccg tatcgtaaag tgaccgacgg tgttgtaact gacgaaattc actacctgtc
     8101 tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac tccaacttgg atgaagaagg
     8161 ccacttcgta gaagacctgg taacttgccg tagcaaaggc gaatccagct tgttcagccg
     8221 cgaccaggtt gactacatgg acgtatccac ccagcaggtg gtatccgtcg gtgcgtccct
     8281 gatcccgttc ctggaacacg atgacgccaa ccgtgcattg atgggtgcga acatgcaacg
     8341 tcaggccgtt ccgactctgc gcgctgataa gccgctggtt ggtactggta tggaacgtgc
     8401 tgttgccgtt gactccggtg taactgcggt agctaaacgt ggtggtgtcg ttcagtacgt
     8461 ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg gtgaagcagg
     8521 tatcgacatc tacaacctga ccaaatacac ccgttctaac cagaacacct gtatcaacca
     8581 gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg cagacggtcc
     8641 gtccaccgac ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt tcatgccgtg
     8701 gaatggttac aacttcgaag actccatcct cgtatccgag cgtgttgttc aggaagaccg
     8761 tttcaccacc atccacattc aggaactggc gtgtgtgtcc cgtgacacca agctgggtcc
     8821 ggaagagatc accgctgaca tcccgaacgt gggtgaagct gcgctctcca aactggatga
     8881 atccggtatc gtttacattg gtgcggaagt gaccggtggc gacattctgg ttggtaaggt
     8941 aacgccgaaa ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg cgatcttcgg
     9001 tgagaaagcc tctgacgtta aagactcttc tctgcgcgta ccaaacggtg tatccggtac
     9061 ggttatcgac gttcaggtct ttactcgcga tggcgtagaa aaagacaaac gtgcgctgga
     9121 aatcgaagaa atgcagctca aacaggcgaa gaaagacctg tctgaagaac tgcagatcct
     9181 cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta gccggtggcg ttgaagctga
     9241 gaagctcgac aaactgccgc gcgatcgctg gctggagctg ggcctgacag acgaagagaa
     9301 acaaaatcag ctggaacagc tggctgagca gtatgacgaa ctgaaacacg agttcgagaa
     9361 gaaactcgaa gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac cgggcgtgct
     9421 gaagattgtt aaggtatatc tggcggttaa acgccgtatc cagcctggtg acaagatggc
     9481 aggtcgtcac ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag atatgcctta
     9541 cgatgaaaac ggtacgccgg tagacatcgt actgaacccg ctgggcgtac cgtctcgtat
     9601 gaacatcggt cagatcctcg aaacccacct gggtatggct gcgaaaggta tcggcgacaa
     9661 gatcaacgcc atgctgaaac agcagcaaga agtcgcgaaa ctgcgcgaat tcatccagcg
     9721 tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac ctgagtacct tcagcgatga
     9781 agaagttatg cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa cgccggtgtt
     9841 cgacggtgcg aaagaagcag aaattaaaga gctgctgaaa cttggcgacc tgccgacttc
     9901 cggtcagatc cgcctgtacg atggtcgcac tggtgaacag ttcgagcgtc cggtaaccgt
     9961 tggttacatg tacatgctga aactgaacca cctggtcgac gacaagatgc acgcgcgttc
    10021 caccggttct tacagcctgg ttactcagca gccgctgggt ggtaaggcac agttcggtgg
    10081 tcagcgtttc ggggagatgg aagtgtgggc gctggaagca tacggcgcag catacaccct
    10141 gcaggaaatg ctcaccgtta agtctgatga cgtgaacggt cgtaccaaga tgtataaaaa
    10201 catcgtggac ggcaaccatc agatggagcc gggcatgcca gaatccttca acgtattgtt
    10261 gaaagagatt cgttcgctgg gtatcaacat cgaactggaa gacgagtaat tctcgctcaa
    10321 acaggtcact gctgtcgggt taaaacccgg cagcggattg tgctaactcc gacgggagca
    10381 aatccgtgaa agatttatta aagtttctga aagcgcagac taaaaccgaa gagtttgatg
    10441 cgatcaaaat tgctctggct tcgccagaca tgatccgttc atggtctttc ggtgaagtta
    10501 aaaagccgga aaccatcaac taccgtacgt tcaaaccaga acgtgacggc cttttctgcg
    10561 cccgtatctt tgggccggta aaagattacg agtgcctgtg cggtaagtac aagcgcctga
    10621 aacaccgtgg cgtcatctgt gagaagtgcg gcgttgaagt gacccagact aaagtacgcc
    10681 gtgagcgtat gggccacatc gaactggctt ccccgactgc gcacatctgg ttcctgaaat
    10741 cgctgccgtc ccgtatcggt ctgctgctcg atatgccgct gcgcgatatc gaacgcgtac
    10801 tgtactttga atcctatgtg gttatcgaag gcggtatgac caacctggaa cgtcagcaga
    10861 tcctgactga agagcagtat ctggacgcgc tggaagagtt cggtgacgaa ttcgacgcga
    10921 agatgggggc ggaagcaatc caggctctgc tgaagagcat ggatctggag caagagtgcg
    10981 aacagctgcg tgaagagctg aacgaaacca actccgaaac caagcgtaaa aagctgacca
    11041 agcgtatcaa actgctggaa gcgttcgttc agtctggtaa caaaccagag tggatgatcc
    11101 tgaccgttct gccggtactg ccgccagatc tgcgtccgct ggttccgctg gatggtggtc
    11161 gtttcgcgac ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt aacaaccgtc
    11221 tgaaacgtct gctggatctg gctgcgccgg acatcatcgt acgtaacgaa aaacgtatgc
    11281 tgcaggaagc ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg atcaccggtt
    11341 ctaacaagcg tcctctgaaa tctttggccg acatgatcaa aggtaaacag ggtcgtttcc
    11401 gtcagaacct gctcggtaag cgtgttgact actccggtcg ttctgtaatc accgtaggtc
    11461 catacctgcg tctgcatcag tgcggtctgc cgaagaaaat ggcactggag ctgttcaaac
    11521 cgttcatcta cggcaagctg gaactgcgtg gtcttgctac caccattaaa gctgcgaaga
    11581 aaatggttga gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt atccgcgaac
    11641 acccggtact gctgaaccgt gcaccgactc tgcaccgtct gggtatccag gcatttgaac
    11701 cggtactgat cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg gcatataacg
    11761 ccgacttcga tggtgaccag atggctgttc acgtaccgct gacgctggaa gcccagctgg
    11821 aagcgcgtgc gctgatgatg tctaccaaca acatcctgtc cccggcgaac ggcgaaccaa
    11881 tcatcgttcc gtctcaggac gttgtactgg gtctgtacta catgacccgt gactgtgtta
    11941 acgccaaagg cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt ctgtatcgct
    12001 ctggtctggc ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat gaaaaagatg
    12061 ctaacggtga attagtagcg aaaaccagcc tgaaagacac gactgttggc cgtgccattc
    12121 tgtggatgat tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg ctgggtaaaa
    12181 aagcaatctc caaaatgctg aacacctgct accgcattct cggtctgaaa ccgaccgtta
    12241 tttttgcgga ccagatcatg tacaccggct tcgcctatgc agcgcgttct ggtgcatctg
    12301 ttggtatcga tgacatggtc atcccggaga agaaacacga aatcatctcc gaggcagaag
    12361 cagaagttgc tgaaattcag gagcagttcc agtctggtct ggtaactgcg ggcgaacgct
    12421 acaacaaagt tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa gcgatgatgg
    12481 ataacctgca aactgaaacc gtgattaacc gtgacggtca ggaagagaag caggtttcct
    12541 tcaacagcat ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca cagattcgtc
    12601 agcttgctgg tatgcgtggt ctgatggcga agccggatgg ctccatcatc gaaacgccaa
    12661 tcaccgcgaa cttccgtgaa ggtctgaacg tactccagta cttcatctcc acccacggtg
    12721 ctcgtaaagg tctggcggat accgcactga aaactgcgaa ctccggttac ctgactcgtc
    12781 gtctggttga cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt acccatgaag
    12841 gtatcatgat gactccggtt atcgagggtg gtgacgttaa agagccgctg cgcgatcgcg
    12901 tactgggtcg tgtaactgct gaagacgttc tgaagccggg tactgctgat atcctcgttc
    12961 cgcgcaacac gctgctgcac gaacagtggt gtgacctgct ggaagagaac tctgtcgacg
    13021 cggttaaagt acgttctgtt gtatcttgtg acaccgactt tggtgtatgt gcgcactgct
    13081 acggtcgtga cctggcgcgt ggccacatca tcaacaaggg tgaagcaatc ggtgttatcg
    13141 cggcacagtc catcggtgaa ccgggtacac agctgaccat gcgtacgttc cacatcggtg
    13201 gtgcggcatc tcgtgcggct gctgaatcca gcatccaagt gaaaaacaaa ggtagcatca
    13261 agctcagcaa cgtgaagtcg gttgtgaact ccagcggtaa actggttatc acttcccgta
    13321 atactgaact gaaactgatc gacgaattcg gtcgtactaa agaaagctac aaagtacctt
    13381 acggtgcggt actggcgaaa ggcgatggcg aacaggttgc tggcggcgaa accgttgcaa
    13441 actgggaccc gcacaccatg ccggttatca ccgaagtaag cggttttgta cgctttactg
    13501 acatgatcga cggccagacc attacgcgtc agaccgacga actgaccggt ctgtcttcgc
    13561 tggtggttct ggattccgca gaacgtaccg caggtggtaa agatctgcgt ccggcactga
    13621 aaatcgttga tgctcagggt aacgacgttc tgatcccagg taccgatatg ccagcgcagt
    13681 acttcctgcc gggtaaagcg attgttcagc tggaagatgg cgtacagatc agctctggtg
    13741 acaccctggc gcgtattccg caggaatccg gcggtaccaa ggacatcacc ggtggtctgc
    13801 cgcgcgttgc ggacctgttc gaagcacgtc gtccgaaaga gccggcaatc ctggctgaaa
    13861 tcagcggtat cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg gttatcaccc
    13921 cggtagacgg tagcgatccg tacgaagaga tgattccgaa atggcgtcag ctcaacgtgt
    13981 tcgaaggtga acgtgtagaa cgtggtgacg taatttccga cggtccggaa gcgccgcacg
    14041 acattctgcg tctgcgtggt gttcatgctg ttactcgtta catcgttaac gaagtacagg
    14101 acgtataccg tctgcagggc gttaagatta acgataaaca catcgaagtt atcgttcgtc
    14161 agatgctgcg taaagctacc atcgttaacg cgggtagctc cgacttcctg gaaggcgaac
    14221 aggttgaata ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac ggcaaagtgg
    14281 gtgcaactta ctcccgcgat ctgctgggta tcaccaaagc gtctctggca accgagtcct
    14341 tcatctccgc ggcatcgttc caggagacca ctcgcgtgct gaccgaagca gccgttgcgg
    14401 gcaaacgcga cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt ctgatcccgg
    14461 caggtaccgg ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg ggtgaagctc
    14521 cggctgcacc gcaggtgact gcagaagacg catctgccag cctggcagaa ctgctgaacg
    14581 caggtctggg cggttctgat aacgagctcg aggtgcacca tcaccatcac cattaaggcc
    14641 tcaaataacg taaaaacccg cttcggcggg tttttttatg gggggagttt agggaaagag
    14701 catttgtcag aataagcttc aatgactgca gacttaagaa ggagattaat gtatggcacg
    14761 cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg tactggtcgc
    14821 cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac cggaagaaaa
    14881 cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca acaaccagat
    14941 cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat tacaagccgt
    15001 taccgctatt gctgaaggtc gtcgttaagc ggccgcactc gagcaccacc accaccacca
    15061 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
    15121 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
    15181 aggaggaact atatccggat
//
