LOCUS       sequence-20038-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Hallows WC, Lee S, Denu JM
  TITLE     Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5.
  PUBMED    16790548
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 5. 103(27):10230-5."
COMMENT     Sequence Label: SIRT7.4
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..30
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(54..68,69..74,75..91,92..98)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    74..90
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    106..122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             141..152
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             171..188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacctcgag aaatcataaa
       61 aaatttattt gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac
      121 aatttcacac agaattcatt aaagaggaga aattaactat gagaggatcg catcaccatc
      181 accatcacgg atccgcatgc atggcagccg ggggtctgag ccgctccgag cgcaaagcgg
      241 cggagcgggt ccggaggttg cgggaggagc agcagaggga gcgcctccgc caggtgtgcg
      301 acgacccgga ggagctgcgg gggaaggtcc gggggctggc cagcgccgtc cggaacgcaa
      361 aatacttggt cgtctacaca ggcgcgggaa tcagcacggc agcgtctatc ccagactacc
      421 ggggccctaa tggagtgtgg acactgcttc agaaagggag aagcgttagt gctgccgacc
      481 tgagcgaggc cgagccaacc ctcacccaca tgagcatcac ccgtctgcat gagcagaagc
      541 tggtgcagca tgtggtgtct cagaactgtg acgggctcca cctgaggagt gggctgccgc
      601 gcacggccat ctccgagctc cacgggaaca tgtacattga agtctgtacc tcctgcgttc
      661 ccaacaggga gtacgtgcgg gtgttcgatg tgacggagcg cactgccctc cacagacacc
      721 agacaggccg gaactgccac aagtgtggga cccagctgc
//
