LOCUS       sequence-20040-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Hallows WC, Lee S, Denu JM
  TITLE     Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5.
  PUBMED    16790548
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 5. 103(27):10230-5."
COMMENT     Sequence Label: ACS2
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..35
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(59..73,74..79,80..96,97..103)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    79..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    111..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             146..157
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             176..193
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      198..207
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tnacttaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcacc tcgagaaatc
       61 ataaaaaatt tatttgcttt gtgagcggat aacaattata atagattcaa ttgtgagcgg
      121 ataacaattt cacacagaat tcattaaaga ggagaaatta actatgagag gatcgcatca
      181 ccatcaccat cacggatcca tggcggcgcg cagcctcggc agcggtgtgg ggcgactgct
      241 gcgtggcctg caagggcgct ctggacaatc ggggtggtca ctgagcgtgt cccgctcgac
      301 cgccacccga ctcccgggct gcgttcctgc agcagcgcaa ccaggctcgt accccgcgtt
      361 gagcgctcag gcagcccagg agccggccgc cttctggggg ccgctggccc gtgacacact
      421 agtttgggac actccttacc atactgtctg ggactgtgac ttcaggacgg gcaagatcgg
      481 ctggttcctg ggaggccagt tgaatgtgtc tgtcaactgc ctggatcagc atgtgcagaa
      541 gtctcctgag accatagctt tgatctggga gagagatgaa cctgggacag aagtgagaat
      601 cacttacagg gagcttcnnn aanaccacat gccgcctggc caatacactg aagagacatg
      661 gagtgcaccg aggggaccgt gtggccatct acatgcctgt gtccccactg gctgtggcag
      721 caatgctggg cctgtgccng gattggngct atccacacag tggnatttgc tggctttant
      781 gcanantcct ngnngggnnn tcangatgcc naatgcaann tntatcactt caccagnant
      841 cngggangnc ntnnnnnng
//
