LOCUS       sequence-200402-       10268 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mouse Fzd8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10268)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd8
FEATURES             Location/Qualifiers
     source          1..10268
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(609..633)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(653..1831)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          662..1600
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(685..705)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    1969..2086
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2129..2731)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2215..2235)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2712..2731
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2763..2959)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(2771..2981)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2777..2912
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(3212..3256)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3441..3674
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4167..4292
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4339..4519
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4520..4746)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4774..4794)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4774..4792)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4813..4829)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4813..4829)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4826..4848)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4837..4853
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4861..4867,4868..4885,4886..4891))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4906..4927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5044..5061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5215..5803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5295..5314)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5974..6765,6766..6834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6597..6616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6835..6939)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6965..7420)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7102..7123)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7314..7333
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7547..7569
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7561..7578
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7562..7578
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7588..7606
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7627..7762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7802..7923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             8001..8234
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    8306..8894
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8412..8423
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8821..8841
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8945..8986)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    8994..9018
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9108..9242
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gctgtggccg ctgccagagg gcggctgctc gccgggagga ggcggcggag gcaggcggcg
       61 cggtgggctg ggcgcggccg tggtgaggtc ggtgcggttg tagtccatgc acagagtgtc
      121 cgggttgccc tgctccggca accgatcgca gcgcatgcgg tcaggccaag caaagccgta
      181 ctggcgcatg agcggcgcgc agccggcctt ggcgcgttca cacacagagc gacaaggcgg
      241 cagaggcttc ttgtagtcct ccaggcagat gggcgtgtac atgctacaca gaaagaactt
      301 gaggtccggg gagcactgta tctccaccag cggccaaaac tggtgcacct ctaggcccgc
      361 ctcatcttgc gtgtcgtggt tgaactggtt gggcatgtaa gtgtagttgt aaccgatgcc
      421 tttgcacaac ggcaccgtga tctcttggca cgccagctcc ttggccgaag ccgcggcagc
      481 gccgctagag cgctgtagca ccgccaaggc ggctaggagc gaggtcactt ccaacaggta
      541 accccactcc atggtaggcc ggcctgaatt cggtaccgga tccagtcgac tgaattggtt
      601 ccggcgccag cctgcttttt tgtacaaact tgttgatcaa tcgatgctag cctcacgaca
      661 cctgaaatgg aagaaaaaaa ctttgaacca ctgtctgagg cttgagaatg aaccaagatc
      721 caaactcaaa aagggcaaat tccaaggaga attacatcaa gtgccaagct ggcctaactt
      781 cagtctccac ccactcagtg tggggaaact ccatcgcata aaacccctcc ccccaaccta
      841 aagacgacgt actccaaaag ctcgagaact aatcgaggtg cctggacggc gcccggtact
      901 ccgtggagtc acatgaagcg acggctgagg acggaaaggc ccttttcctt tgtgtgggtg
      961 actcacccgc ccgctctccc gagcgccgcg tcctccattt tgagctccct gcagcagggc
     1021 cgggaagcgg ccatctttcc gctcacgcaa ctggtgccga ccgggccagc cttgccgccc
     1081 agggcggggc gatacacggc ggcgcgaggc caggcaccag agcaggccgg ccagcttgag
     1141 actacccccg tccgattctc ggtggccgcg ctcgcaggcc ccgcctcgcc gaacatgtgc
     1201 gctgggacgc acgggccccg tcgccgcccg cggccccaaa aaccgaaata ccagtgtgca
     1261 gatcttggcc cgcatttaca agactatctt gccagaaaaa aagcgtcgca gcaggtcatc
     1321 aaaaatttta aatggctaga gacttatcga aagcagcgag acaggcgcga aggtgccacc
     1381 agattcgcac gcggcggccc cagcgcccag gccaggcctc aactcaagca cgaggcgaag
     1441 gggctcctta agcgcaaggc ctcgaactct cccacccact tccaacccga agctcgggat
     1501 caagaatcac gtactgcagc caggtggaag taattcaagg cacgcaaggg ccataacccg
     1561 taaagaggcc aggcccgcgg gaaccacaca cggcacttac ctgtgttctg gcggcaaacc
     1621 cgttgcgaaa aagaacgttc acggcgacta ctgcacttat atacggttct cccccaccct
     1681 cgggaaaaag gcggagccag tacacgacat cactttccca gtttaccccg cgccaccttc
     1741 tctaggcacc ggttcaattg ccgacccctc cccccaactt ctcggggact gtgggcgatg
     1801 tgcgctctgc ccactgacgg gcaccggagc caattcccac tcctttcaag acctagaagg
     1861 tccattagct gcaaagattc ctctctgttt aaaactttat ccatctttgc acctgcaggc
     1921 ctcgagccgg cgccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat
     1981 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     2041 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     2101 tgccatttgt ctcaagatct agaattcgtt aactggcacc gggcttgcgg gtcatgcacc
     2161 aggtgcgcgg tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt
     2221 agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg
     2281 ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg
     2341 agacgccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc
     2401 cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga
     2461 tctcggcgaa caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccacggcgg
     2521 cgccgtcgtc cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt
     2581 cttgcagctc ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg cgcgtggcgg
     2641 ggtagtcggc gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg tcgcgggtgg
     2701 cgaggcgcac cgtgggcttg tactcggtca tggtaagctc cggtggtacc tctagaaagc
     2761 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     2821 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     2881 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     2941 gctgactaat tgagacgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     3001 ccacacctgg ttgctgacta attgagatgc aatgcatggg tcccctcggg gttgggaggt
     3061 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag
     3121 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc
     3181 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata
     3241 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc
     3301 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt
     3361 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc
     3421 cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga
     3481 ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct
     3541 caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct
     3601 gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa
     3661 ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct
     3721 tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc
     3781 tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg
     3841 gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt
     3901 atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt
     3961 tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct
     4021 gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact
     4081 atatgtttta atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc
     4141 gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct
     4201 ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc
     4261 gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag
     4321 tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc
     4381 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     4441 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     4501 ggtctaacca gagagacccg tttatgtatc gagctaggca cttaaataca atatctctgc
     4561 aatgcggcaa ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc cttcctaata
     4621 aggcacgatc gtaccacctt acttccacca atcggcatgc acggtgcttt ttctctcctt
     4681 gtaaggcatg ttgctaactc atcgttacca tgttgcaaga ctacaagagt attgcataag
     4741 actacattaa gcttgcagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     4801 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4861 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4921 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4981 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5401 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5641 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5881 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5941 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6001 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6061 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6121 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6181 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6241 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6301 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6361 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6421 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6481 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6541 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6601 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6661 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6721 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6781 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6841 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6901 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6961 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7021 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     7081 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     7141 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     7201 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     7261 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     7321 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     7381 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     7441 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     7501 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     7561 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacg
     7621 tccctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     7681 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     7741 tgggcggagt taggggcggg atgggcggag ttaggggcgg gatagctaga gccagacatg
     7801 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     7861 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     7921 gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
     7981 acatgaacta ctatacgtac tgctagagat tttccacact gactaaaagg gtctgaggga
     8041 tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa
     8101 gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat
     8161 ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg
     8221 aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt
     8281 gtaagtcatt ggtcttaaag tcgacgcggg gaggcggccc aaagggagat ccgactcgtc
     8341 tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga
     8401 aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag
     8461 gtggcaacac aggcgagcag ccaaggaaag gacgatgatt tccccgacaa caccacggaa
     8521 ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt
     8581 tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg
     8641 gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc
     8701 ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag
     8761 aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca
     8821 catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa
     8881 tccagaggtt gattgtcgac ttaacgcgtt gattgtcgac ttaaccggta ctagttcatt
     8941 actacgtaga atcgagaccg aggagagggt tagggatagg cttaccgata tcaaccactt
     9001 tgtacaagaa agctgggtct agatatctcg agtgcggccg cgaattaaaa aacctcccac
     9061 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     9121 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     9181 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     9241 tctacgtaat acgactcact atagttctag aggcgcgcct cagacctggg acaatggcat
     9301 ttgcttaggg taagatacag agctggccgt gccagaccgc cacgtcaggc cggtactgac
     9361 gtcgctgtag agggatcccc cgcctccgcc gtgtccccca cctccgccgg gcccgggtcc
     9421 actgccccca gggccgctgc ctccagcgcc cgcgcctact gcagcgccct tgctggccca
     9481 gcagcaccta gtgcacaacg cgcgccagga ctccagagtc ttgccggacc agacccacac
     9541 gcccgatgtg atgcccacta ctaggcacat gaagtacttg agcatgaaga ccgcgtaatc
     9601 gggcctgcga gcctggtccg gttgcaggtc ccgaaggcat gggcagttgt gcgtggcctc
     9661 ccagcgcggt cggttgtgct gctcatagaa aaggcaggcg acaacgacgg cagcgggcac
     9721 cgtgtagagc acggtgaaga ggcccaagcg gatcatgagt ttttctagct tgtgtgtctt
     9781 agttggacct ccttgctgct tgatgactga acggattcgg aacagcgaca cgaagccagc
     9841 taacagaaac atagtcccaa tgaagaggta gataaccagt ggcgccagca caaagccgcg
     9901 taggttgtca aggctctggt tgcccacgta gcagatgccc gccaccgggt cgccgtctac
     9961 ggagctgagc gccagcaccg cgatggactt gacgctgggc acaagccacg cggccaggtg
    10021 gaagtactgc gagtagcctg ctatggcctc gttaccccac ttcatgccag ctgccaagaa
    10081 ccacgtgagc gacaggatta cccaccagat ggagctggcc atgccaaaaa agtagacaag
    10141 gagaaagacc accgtgcaca gcgcggggcc agtggtctca tagcgaacat gctgctcaac
    10201 tgcgcccagc tcctcgtact cgccgcgcgc gcccgggctg ctcgcccccg ctcccgctgc
    10261 ccctgcgc
//
