LOCUS       sequence-200407-       10369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9 plasmid expressing full length mKate2 mRNA without RBS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Galizi R, Duncan JN, Rostain W, Quinn CM, Storch M, Kushwaha M, 
            Jaramillo A
  TITLE     Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and
            Diagnostics.
  JOURNAL   CRISPR J. 2020 Oct;3(5):398-408. doi: 10.1089/crispr.2020.0029.
  PUBMED    33095053
REFERENCE   2  (bases 1 to 10369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2020"; journalName: "CRISPR J"; date: "2020-10"; 
            volume: "3"; issue: "5"; pages: "398-408"
COMMENT     Sequence Label: PAJ408
FEATURES             Location/Qualifiers
     source          1..10369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    179..197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    204..222
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(1229..1246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1363..1908)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1443..1462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(2020..2025,2026..2042,2043..2048)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(2366..2444)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             join(2740..2766,2767..2769,2770..5256,5257..5259,
                     5260..6846)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    6867..6998
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   6999..7034
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   7065..7100
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(7529..7548)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(9401..10060)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     9975..9994
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(10061..10163)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gttcagctac tgacagcttg attctagctg atcgtggacc ggaaggtgag ccagtgagtt
       61 gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatgc gaccgccgga
      121 gggttgcgtt tgagacgggc gacagatcga cactgctcga tccgctcgca ccgaattctc
      181 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc acatggtaag
      241 cgaactgatc aaggagaata tgcatatgaa actgtacatg gaaggtacgg taaataacca
      301 ccacttcaag tgcacctccg agggcgaagg taaaccgtat gagggtactc agaccatgcg
      361 tatcaaagca gtggaaggcg gccctctgcc gtttgcattc gacatcctgg cgacgtcctt
      421 catgtacggc agcaaaactt tcattaacca tacccagggt attccggact tcttcaaaca
      481 atctttcccg gaaggcttta cctgggaacg tgtgactact tacgaagatg gtggcgtact
      541 gaccgccact caagatacca gcctgcagga cggttgtctg atctacaacg taaaaatccg
      601 tggtgttaat ttcccgtcca acggcccggt aatgcagaag aaaactctgg gctgggaagc
      661 gtccactgaa acgctgtacc cggctgacgg tggtctggaa ggccgcgcgg atatggcact
      721 gaaactggtt ggtggcggcc acctgatctg caacctgaag actacctacc gttctaaaaa
      781 gcctgctaag aacctgaaga tgcctggcgt ctattacgtt gatcgtcgtc tggaacgtat
      841 caaagaggca gacaaagaaa cctacgttga acagcatgaa gtcgcggttg cacgctactg
      901 cgatctgcct tctaagctgg gtcaccgtta ataaatcctt agcgaaagct aaggattttt
      961 tttctgcagg gatcgacgag agcagcgcga ctggatcagt tctggacgag cgagctgtcg
     1021 tccgacccgt gatcttacgg cattatacgt atgatcgggg gtggtgcgta acggcaaaag
     1081 caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag
     1141 ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga
     1201 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
     1261 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     1321 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     1381 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     1441 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
     1501 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
     1561 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1621 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
     1681 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
     1741 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
     1801 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
     1861 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
     1921 attaatcaga taaaatattt ctagatttca gtgcaattta tctcttcaaa tgtagcacct
     1981 gaagtcagcc ccatacgata taagttgtaa ttctaatgtt tgacagctta tcatcgataa
     2041 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
     2101 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     2161 cttggttatg ccggtactgc cgggcctctt gcgggattac gaaatcatcc tgtggagctt
     2221 agtaggttta gcaagatggc agcgcctaaa tgtagaatga taaaaggatt aagagattaa
     2281 tttccctaaa aatgataaaa caagcgtttt gaaagcgctt gtttttttgg tttgcagtca
     2341 gagtagaata gaagtatcaa aaaaagcacc gactcggtgc cactttttca agttgataac
     2401 ggactagcct tattttaact tgctatgctg ttttgaatgg ttccaacaag attattttat
     2461 aacttttata acaaataatc aaggagaaat tcaaagaaat ttatcagcca taaaacaata
     2521 cttaatacta tagaatgata acaaaataaa ctacttttta aaagaatttt gtgttataat
     2581 ctatttatta ttaagtattg ggtaatattt tttgaagaga tattttgaaa aagaaaaatt
     2641 aaagcatatt aaactaattt cggaggtcat taaaactatt attgaaatca tcaaactcat
     2701 tatggattta atttaaactt tttattttag gaggcaaaaa tggataagaa atactcaata
     2761 ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt
     2821 ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt
     2881 ataggggctc ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca
     2941 gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca
     3001 aatgagatgg cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg
     3061 gaagaagaca agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct
     3121 tatcatgaga aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat
     3181 aaagcggatt tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat
     3241 tttttgattg agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag
     3301 ttggtacaaa cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat
     3361 gctaaagcga ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct
     3421 cagctccccg gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt
     3481 ttgaccccta attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca
     3541 aaagatactt acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct
     3601 gatttgtttt tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga
     3661 gtaaatactg aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa
     3721 catcatcaag acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat
     3781 aaagaaatct tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct
     3841 agccaagaag aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag
     3901 gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac
     3961 ggctctattc cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa
     4021 gacttttatc catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga
     4081 attccttatt atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg
     4141 aagtctgaag aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca
     4201 gctcaatcat ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta
     4261 ctaccaaaac atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc
     4321 aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc
     4381 attgttgatt tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat
     4441 tatttcaaaa aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt
     4501 aatgcttcat taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg
     4561 gataatgaag aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa
     4621 gatagggaga tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg
     4681 atgaaacagc ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt
     4741 aatggtatta gggataagca atctggcaaa acaatattag attttttgaa atcagatggt
     4801 tttgccaatc gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac
     4861 attcaaaaag cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta
     4921 gctggtagcc ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg
     4981 gtcaaagtaa tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat
     5041 cagacaactc aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt
     5101 atcaaagaat taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa
     5161 aatgaaaagc tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa
     5221 ttagatatta atcgtttaag tgattatgat gtcgatgcca ttgttccaca aagtttcctt
     5281 aaagacgatt caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg
     5341 gataacgttc caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta
     5401 aacgccaagt taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt
     5461 ttgagtgaac ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc
     5521 actaagcatg tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat
     5581 aaacttattc gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga
     5641 aaagatttcc aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg
     5701 tatctaaatg ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag
     5761 tttgtctatg gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa
     5821 gaaataggca aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa
     5881 acagaaatta cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg
     5941 gaaactggag aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg
     6001 tccatgcccc aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag
     6061 gagtcaattt taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat
     6121 ccaaaaaaat atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct
     6181 aaggtggaaa aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca
     6241 attatggaaa gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat
     6301 aaggaagtta aaaaagactt aatcattaaa ctacctaaat atagtctttt tgagttagaa
     6361 aacggtcgta aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct
     6421 ctgccaagca aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt
     6481 agtccagaag ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat
     6541 gagattattg agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta
     6601 gataaagttc ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa
     6661 aatattattc atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt
     6721 gatacaacaa ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactctt
     6781 atccatcaat ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt
     6841 gactgaagta tattttagat gaagattatt tcttaataac taaaaatatg gtataatact
     6901 cttaataaat gcagtaatac aggggctttt caagactgaa gtctagctga gacaaatagt
     6961 gcgattacga aattttttag acaaaaatag tctacgaggt tttagagcta tgctgttttg
     7021 aatggtccca aaactgagac cagtctcgga agctcaaagg tctcgtttta gagctatgct
     7081 gttttgaatg gtcccaaaac ttcagcacac tgagacttgt tgagttccat gttttagagc
     7141 tatgctgttt tgaatggact ccattcaaca ttgccgatga taacttgaga aagagggtta
     7201 ataccagcag tcggatacct tcctattctt tctgttaaag cgttttcatg ttataatagg
     7261 caaaagaaga gtagtgtgat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
     7321 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
     7381 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
     7441 atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc
     7501 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     7561 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     7621 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     7681 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     7741 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     7801 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     7861 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     7921 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     7981 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     8041 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     8101 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     8161 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     8221 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     8281 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     8341 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca
     8401 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc
     8461 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg
     8521 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc
     8581 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt
     8641 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt
     8701 tccgtgtttc gtaaagtctg gaaacgcgga agtcccctac gtgctgctga agttgcccgc
     8761 aacagagagt ggaaccaacc ggtgatacca cgatactatg actgagagtc aacgccatga
     8821 gcggcctcat ttcttattct gagttacaac agtccgcacc gctgccggta gctccttccg
     8881 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     8941 cgtaggacag gtgccggcag cgcccaacag tcccccggcc acggggcctg ccaccatacc
     9001 cacgccgaaa caagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
     9061 gctaccctgt ggaacaccta catctgtatt aacgaagcgc taaccgtttt tatcaggctc
     9121 tgggaggcag aataaatgat catatcgtca attattacct ccacggggag agcctgagca
     9181 aactggcctc aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg
     9241 gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg
     9301 tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     9361 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     9421 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     9481 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     9541 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     9601 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     9661 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     9721 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     9781 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     9841 ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     9901 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     9961 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
    10021 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
    10081 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
    10141 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cggggcttcc
    10201 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
    10261 tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg
    10321 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcct
//
