LOCUS       sequence-20041-e         723 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Hallows WC, Lee S, Denu JM
  TITLE     Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5.
  PUBMED    16790548
REFERENCE   2  (bases 1 to 723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 5. 103(27):10230-5."
COMMENT     Sequence Label: ACS2 Truncated
FEATURES             Location/Qualifiers
     source          1..723
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..33
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(57..71,72..77,78..94,95..101)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    77..93
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    109..125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             144..155
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             174..191
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acttaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagaaatcat
       61 aaaaaattta tttgctttgt gagcggataa caattataat agattcaatt gtgagcggat
      121 aacaatttca cacagaattc attaaagagg agaaattaac tatgagagga tcgcatcacc
      181 atcaccatca cggatccccg ggctgcgttc ctgcagcagc gcaaccaggc tcgtaccccg
      241 cgttgagcgc tcaggcagcc caggagccgg ccgccttctg ggggccgctg gcccgtgaca
      301 cactagtttg ggacactcct taccatactg tctgggactg tgacttcagg acgggcaaga
      361 tcggctggtt cctgggaggc cagttgaatg tgtctgtcaa ctgcctggat cagcatgtgc
      421 agaagtctcc tgagaccata gctttgatct gggagagaga tgaacctggg acagaagtga
      481 gaatcactta cagggagctt ctggagacca catgccgcct ggccaataca ctgaagagac
      541 atggagtgca ccgaggggac cgtgtggcca tctacatgcc tgtgtcccca ctggctgtgg
      601 cagcaatgct ggcctgtgcc aggattggag ctatccacac agtggtattt gctggcttta
      661 gtgcagagtc cttggctggg angatcaatg atgccaaatg caaggctgtt atcaccttca
      721 acc
//
