LOCUS       sequence-20042-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Hallows WC, Lee S, Denu JM
  TITLE     Sirtuins deacetylate and activate mammalian acetyl-CoA synthetases.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Jul 5. 103(27):10230-5.
  PUBMED    16790548
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2006 Jul 5. 103(27):10230-5."
COMMENT     Sequence Label: ACS2 Truncated
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(188..292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     360..378
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(402..416,417..422,423..439,440..446)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    422..438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    454..470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             489..500
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             519..536
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
       61 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      301 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
      361 ataggcgtat cacgaggccc tttcgtcttc acctcgagaa atcataaaaa atttatttgc
      421 tttgtgagcg gataacaatt ataatagatt caattgtgag cggataacaa tttcacacag
      481 aattcattaa agaggagaaa ttaactatga gaggatcgca tcaccatcac catcacggat
      541 ccccgggctg cgttcctgca gcagcgcaac caggctcgta ccccgcgttg agcgctcagg
      601 cagccaggag c
//
