LOCUS       sequence-200429-        3911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding DsRed-Express.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-DsRed-Express
FEATURES             Location/Qualifiers
     source          1..3911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      838..884
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1057..2007)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2055..2277
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2783..2817)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2858..3652)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2969..2988)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3579..3598
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3831..3849
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggttgctgga taactttacg ggcatgcata aggctcgtat gatatattca gggagaccac
       61 aacggtttcc ctctacaaat aattttgttt aacttttcta gatttaagaa ggagatatac
      121 atatggccag cagcgaggat gtgattaaag agttcatgcg cttcaaagtt cgcatggagg
      181 gttcggttaa cggtcacgag ttcgagatcg aaggcgaagg cgagggccgt ccgtatgaag
      241 gcactcaaac cgcgaaactg aaagtgacta aaggcggccc gctgcctttt gcgtgggaca
      301 tcctgagccc gcaatttcag tacggttcta aagtgtatgt taaacaccca gcggatatcc
      361 cggactataa aaagctgtct tttccggaag gtttcaagtg ggaacgcgta atgaattttg
      421 aagatggtgg tgtcgtgacc gtcactcagg actcctccct gcaggatggc agcttcatct
      481 ataaagttaa atttattggt gtgaattttc catctgatgg cccggtgatg cagaaaaaga
      541 cgatgggttg ggaggcgtct accgaacgcc tgtatccgcg cgatggtgtg ctgaaaggcg
      601 aaattcataa agcgctgaaa ctgaaagatg gcggccatta tctggtggaa tttaaaagca
      661 tttacatggc caagaaacct gtgcagctgc ctggctatta ctatgtggat tccaaactgg
      721 acatcacctc tcataatgaa gattatacga tcgtagagca atatgagcgc gccgagggtc
      781 gtcatcatct gtttctgtaa atgtccagac ctgcaggcat gcaagctcta gaggcatcaa
      841 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg aaatgcacca
      901 aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc tgtgcatatg
      961 gacagttttc cctttgatat ctaacggtga acagttgttc tacttttgtt tgttagtctt
     1021 gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt atttagccag
     1081 tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc
     1141 atgcttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc
     1201 agttaaagca tcgtgtagtg tttttcttag tccgttacgt aggtaggaat ctgatgtaat
     1261 ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag
     1321 atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt
     1381 atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac
     1441 ccattggtta agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa
     1501 ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa
     1561 taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt
     1621 atattttatg aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa
     1681 ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt
     1741 aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa
     1801 tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa
     1861 cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca
     1921 gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt
     1981 tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact
     2041 ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt gagttgtggg
     2101 tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt
     2161 aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag
     2221 aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact
     2281 actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca
     2341 aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct
     2401 gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc
     2461 agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt
     2521 tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag
     2581 ggcgttttat ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt cagcagttcc
     2641 tgccctctga ttttccagtc tgaccacttc ggattatccc gtgacaggtc attcagactg
     2701 gctaatgcac ccagtaaggc agcggtatca tcaacaggct tacccgtctt actgtcaacc
     2761 agacccgcca ggattctcat gcataaaaaa cgcccggcgg caaccgagcg ttctgaatta
     2821 attaatcatc gcgactgcag agatcccaga gtccttatta gaagaactcg tcaagaaggc
     2881 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     2941 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     3001 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     3061 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     3121 tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     3181 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     3241 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     3301 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     3361 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     3421 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat
     3481 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     3541 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     3601 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     3661 atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca
     3721 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
     3781 gcaattccga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3841 tcacgaggcc ctttcgtctt caccacagct aacaccacgt cgtccctatc tgctgcccta
     3901 ggtctatgag t
//
