LOCUS       sequence-20043-e         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Boatright KM, Deis C, Denault JB, Sutherlin DP, Salvesen GS
  TITLE     Activation of caspases-8 and -10 by FLIP(L).
  JOURNAL   Biochem J. 2004 Sep 1. 382(Pt 2):651-7.
  PUBMED    15209560
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Sep 1. 382(Pt 2):651-7."
COMMENT     Sequence Label: Delta DED FLIP-L
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..70
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             80..97
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatatacc atgaaacacc accatcatca
       61 tcatcatcac agcagcggcc tggtgccgcg cggcagccat atgcaagcag caatccaaaa
      121 gagtctcaag gatccttcaa ataacttcag gctccataat gggagaagta aagaacaaag
      181 acttaaggaa cagcttggcg ctcaacaaga accagtgaag aaatccattc aggaatcaga
      241 agcttttttg cctcagagca tacctgaaga gagatacaag atgaagagca agcccctagg
      301 aatctgcctg ataatcgatt gcattggcaa tgagacagag cttcttcgag acaccttcac
      361 ttccctgggc tatgaagtcc agaaattctt gcatctcagt atgcatggta tatcccagat
      421 tcttggccaa tttgcctgta tgcccgagca ccgagactac gacagctttg tgtgtgtcct
      481 ggtgagccga ggaggctccc agagtgtgta tggtgtggat cagactcact cagggctccc
      541 cctgcatcac atcaggagga tgttcatggg agattcatgc ccttatctag cagggaagcc
      601 aaagatgttt tttattcaga actatgtggt gtcagagggc cagctggagg acagcagcct
      661 cttggaggtg gatgggccag cga
//
