LOCUS       sequence-200430-        3626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  to make in situ RNA probes for Tmeff2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Huang H, Teng P, Mei R, Yang A, Zhang Z, Zhao X, Qiu M
  TITLE     Tmeff2 is expressed in differentiating oligodendrocytes but 
            dispensable for their differentiation in vivo.
  JOURNAL   Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1038/s41598-017-00407-1.
  PUBMED    28336932
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-23"; volume: "7(1):337. doi"; pages: " 
            10.1038/s41598-017-00407-1"
COMMENT     Sequence Label: pT7T3D-PacI-Tmeff2
FEATURES             Location/Qualifiers
     source          1..3626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        169..187
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(986..1005)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(987..1005)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1019..1035)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1019..1035)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1032..1054)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1043..1059
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1067..1073,1074..1091,1092..1097))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1112..1133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1250..1267)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1421..2009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1501..1520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2180..2971,2972..3040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIIVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2803..2822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3041..3145)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3171..3626)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3308..3329)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3520..3539
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctaaaa ttaaccctca
      181 ctaaagggaa taagcttgcg gccgcgacat ccacctgtga catttgccag tttggggcag
      241 agtgtgatga agatgctgag gatgtctggt gtgtgtgcaa catagactgt tctcaaacca
      301 acttcaatcc cctctgtgct tctgatggga aatcttatga taatgcatgt caaatcaaag
      361 aagcgtcatg tcagaaacag gagaaaattg aagtcatgtc tctgggtcga tgtcaagata
      421 acacaactac aaccactaaa tctgaagatg ggcattatgc aagaacagat tatgcagaga
      481 atgctaacaa attagaagaa agtgccagag aacaccacat accttgccca gaacattata
      541 atggtttctg catgcatggg aagtgtgaac actccatcaa tatgcaggag ccatcttgca
      601 gatgcgatgc tggttacact ggtcaacact gtgaaaagaa ggactacagt gttctctacg
      661 ttgttcctgg ccctgtccgg ttccagtacg tcttgattgc agctgtgatt ggaacaattc
      721 agattgctgt catctgcgtg gtggtcctct gcatcacaag gaaatgcccc agaagcaaca
      781 gaattcacag acagaagcaa aatacagggc actacagttc agacaacaca acaagagcat
      841 ccacacggtt aatctagagg gagcatgttt cccagtggcc ggacaaccaa cagcttggac
      901 tacacaatac agtattagag acaaaagaat aagacaagat ctacacatgt tgccttgcgt
      961 ttgtggtaat ctcgagggcc aaattcccta tagtgagtcg tattaaattc gtaatcatgt
     1021 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     1081 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     1141 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     1201 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     1261 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     1321 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     1381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     1441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1861 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2101 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2161 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact atgatacggg
     2281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     2341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     2401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     2461 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     2521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     2581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     2641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     2701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     2761 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     2821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     2881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     3181 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     3241 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     3301 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     3361 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     3421 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     3481 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     3541 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     3601 gcttaatgcg ccgctacagg gcgcgt
//
