LOCUS       sequence-20044-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Boatright KM, Deis C, Denault JB, Sutherlin DP, Salvesen GS
  TITLE     Activation of caspases-8 and -10 by FLIP(L).
  JOURNAL   Biochem J. 2004 Sep 1. 382(Pt 2):651-7.
  PUBMED    15209560
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem J. 
            2004 Sep 1. 382(Pt 2):651-7."
COMMENT     Sequence Label: Delta DED FLIP-L D/A
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             41..64
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgaaa caccaccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag ccatatgcaa gcagcaatcc aaaagagtct
      121 caaggatcct tcaaataact tcaggctcca taatgggaga agtaaagaac aaagacttaa
      181 ggaacagctt ggcgctcaac aagaaccagt gaagaaatcc attcaggaat cagaagcttt
      241 tttgcctcag agcatacctg aagagagata caagatgaag agcaagcccc taggaatctg
      301 cctgataatc gattgcattg gcaatgagac agagcttctt cgagacacct tcacttccct
      361 gggctatgaa gtccagaaat tcttgcatct cagtatgcat ggtatatccc agattcttgg
      421 ccaatttgcc tgtatgcccg agcaccgaga ctacgacagc tttgtgtgtg tcctggtgag
      481 ccgaggaggc tcccagagtg tgtatggtgt ggatcagact cactcagggc tccccctgca
      541 tcacatcagg aggatgttca tgggagattc atgcccttat ctagcaagga agccaaagat
      601 gttttttatt caaaactatg tggtgtcaga gggccagctg gaggacagca gcctcttgga
      661 ggtggctggg ccagcgatga agaatgtgga gttcaaggct cataa
//
