LOCUS       sequence-200450-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses thermostable variant of human carbonic anhydrase II with 
            TAG stop codon at site 20 and C-terminal 6x his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Mehl R, van Zee K, Kean K
  TITLE     
  JOURNAL   2020
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "2020"
COMMENT     Sequence Label: pBad-CA TAG20
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             781..798
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggcgcatc attggggtta cggtaaacac aacggtccgg agcattggca caaagattag
       61 ccaattgcga agggcgaacg tcaaagcccg gttgacattg atacgcacac ggcaaagtac
      121 gacccgagcc tgaaaccgct gagcgtttcc tatgaccagg ctacgagcct gcgtatcctg
      181 aacaatggcc acaccttcaa cgtggagttt gatgattccc aagataaggc ggttctgaaa
      241 ggtggtccgt tggatggcac ctaccgcctg atccaatttc actttcactg gggtagccac
      301 gacggtcagg gcagcgagca taccgtggac aaaaagaagt atgcagccga actgcacctg
      361 gtgcattgga acacgaagta cggcgacttc ggtaaagcgg tccagcaacc ggacggtctg
      421 gctgttctgg gtattttcct gaaggtcggc agcgcgaacc cgggtctgca gaaagtggtt
      481 gacgtgttgg actctatcaa gaccaaaggc aagagcgcgg acttcaccaa tttcgatccg
      541 cgtggtctgc tgccggagag cctggattac tggacttatc cgggcagcct gaccaccccg
      601 ccattgctgg agtgcgtgac ctggatcgtc ttgaaagaac cgatcagcgt tagctctgaa
      661 caggtcagca agttccgcaa gctgaatttc aatggtgagg gcgagccgga agaaccgatg
      721 gtcgataatt ggcgtcctac ccaaccgctg aaaaaccgcc agattaaagc atcctttaag
      781 catcaccacc accatcacta aa
//
