LOCUS       sequence-200457-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding comVenME.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-comVenME
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      900..986
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1524..2474)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2522..2744
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3236..3330)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3361..4155)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3472..3491)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4082..4101
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4334..4352
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctgctgccc taggtctatg agtggttgct ggataacttt acgggcatgc ataaggctcg
       61 tatgatatat tcagggagac cacaacggtt tccctctaca aataattttg tttaactttt
      121 ctagatttaa gaaggagata tacatatgag caaaggtgaa gaactgttca ccggcgttgt
      181 gccaattctg gttgagctgg atggtgacgt gaatggccac aaattttccg tgtctggtga
      241 aggcgagggt gatgctactt atggcaaact gactctgaaa ctgatctgta ccaccggcaa
      301 actgcctgtt ccgtggccaa ctctggtcac tactctgggt tacggcctga tgtgttttgc
      361 gcgttacccg gatcacatga aacagcatga cttcttcaaa tctgccatgc cggaaggcta
      421 tgtccaagaa cgtacgatct ttttcaagga cgacggcaac tataaaaccc gtgccgaagt
      481 taaattcgag ggtgacaccc tggttaaccg catcgaactg aaaggcattg acttcaaaga
      541 ggacggcaac attctgggtc acaagctgga atacaactac aactcccaca acgtttacat
      601 tactgctgac aagcagaaaa acggcatcaa agcaaacttc aagatccgtc acaacattga
      661 agatggtggc gtacagctgg cagatcacta ccagcagaac actccaatcg gtgatggccc
      721 agtactgctg ccagataacc attacctgtc ctaccagagc aaactgtcta aagacccgaa
      781 cgaaaaacgt gaccacatgg tactgctgga atttgttacc gcggcaggca ttacccacgg
      841 tatggacgaa ctgtataaat aaatgtccag acctgcaggc atgcaagctc tagaggcatc
      901 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
      961 tgaacgctct cctgagtagg acaaatccgc cgccctagac ctagggtacg ggttttgctg
     1021 cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga tccggcttca
     1081 gccggtttgc cggctgaaag cgctatttct tccagaattg ccatgatttt ttccccacgg
     1141 gaggcgtcac tggctcccgt gttgtcggca gctttgattc gataagcagc atcgcctgtt
     1201 tcaggctgtc tatgtgtgac tgttgagctg taacaagttg tctcaggtgt tcaatttcat
     1261 gttctagttg ctttgtttta ctggtttcac ctgttctatt aggtgttaca tgctgttcat
     1321 ctgttacatt gtcgatctgt tcatggtgaa cagctttgaa tgcaccaaaa actcgtaaaa
     1381 gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac agttttccct
     1441 ttgatatgta acggtgaaca gttgttctac ttttgtttgt tagtcttgat gcttcactga
     1501 tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat gttctctagt
     1561 gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata cttactttgc
     1621 atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg
     1681 tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt tgttggtatt
     1741 ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta
     1801 tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat
     1861 ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc
     1921 cttttaaact catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta
     1981 atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa
     2041 atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat
     2101 ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg
     2161 cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac caaaggattc
     2221 ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac accataagca
     2281 ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga taccgtccgt
     2341 tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc
     2401 ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca
     2461 actaattcag acatacatct caattggtct aggtgatttt aatcactata ccaattgaga
     2521 tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat ctgtaaattc
     2581 tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga
     2641 cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa
     2701 taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt
     2761 ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa
     2821 aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa tattcctttt
     2881 gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt tcgctgcgct
     2941 cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat ggattcatgc
     3001 aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc gttttatggc
     3061 gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc cctctgattt
     3121 tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct aatgcaccca
     3181 gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctagtg cttggattct
     3241 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     3301 aggtcattac tggatctatc aacaggagtc caagcgagct ctcgaacccc agagtcccgc
     3361 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     3421 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     3481 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     3541 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     3601 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     3661 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     3721 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     3781 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     3841 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     3901 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     3961 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     4021 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     4081 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     4141 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct
     4201 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     4261 cttaccagag ggcgccccag ctggcaattc cgacgtctaa gaaaccatta ttatcatgac
     4321 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaccaca gctaacacca
     4381 cgtcgtccct a
//
