LOCUS       sequence-200462-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding comVenJBC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-comVenJBC
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..42
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      981..1067
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1605..2555)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2603..2825
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3317..3411)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3442..4236)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3553..3572)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4163..4182
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaccaca
       61 gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc tggataactt
      121 tacgggcatg cataaggctc gtatgatata ttcagggaga ccacaacggt ttccctctac
      181 aaataatttt gtttaacttt tctagattta agaaggagat atacatatga gcaaaggtga
      241 agaactgttc accggcgttg tgccaattct ggttgagctg gatggtgacg tgaatggcca
      301 caaattttcc gtgtctggtg aaggcgaggg tgatgctact tatggcaaac tgactctgaa
      361 actgatctgt accaccggca aactgcctgt tccgtggcca actctggtca ctactctggg
      421 ttacggcctg cagtgttttg cgcgttaccc ggatcacatg aaacagcatg acttcttcaa
      481 atctgccatg ccggaaggct atgtccaaga acgtacgatc tttttcaagg acgacggcaa
      541 ctataaaacc cgtgccgaag ttaaattcga gggtgacacc ctggttaacc gcatcgaact
      601 gaaaggcatt gacttcaaag aggacggcaa cattctgggt cacaagctgg aatacaacta
      661 caactcccac aacgtttaca ttactgctga caagcagaaa aacggcatca aagcaaactt
      721 caagatccgt cacaacattg aagatggtgg cgtacagctg gcagatcact accagcagaa
      781 cactccaatc ggtgatggcc cagtactgct gccagataac cattacctgt cctaccagag
      841 caaactgtct aaagacccga acgaaaaacg tgaccacatg gtactgctgg aatttgttac
      901 cgcggcaggc attacccacg gtatggacga actgtataaa taaatgtcca gacctgcagg
      961 catgcaagct ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     1021 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga
     1081 cctagggtac gggttttgct gcccgcaaac gggctgttct ggtgttgcta gtttgttatc
     1141 agaatcgcag atccggcttc agccggtttg ccggctgaaa gcgctatttc ttccagaatt
     1201 gccatgattt tttccccacg ggaggcgtca ctggctcccg tgttgtcggc agctttgatt
     1261 cgataagcag catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt
     1321 gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca cctgttctat
     1381 taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga acagctttga
     1441 atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg
     1501 tgcatatgga cagttttccc tttgatatgt aacggtgaac agttgttcta cttttgtttg
     1561 ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat
     1621 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca
     1681 ttgagatcat acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga
     1741 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct
     1801 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg
     1861 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg
     1921 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt
     1981 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg
     2041 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt
     2101 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt
     2161 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta
     2221 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca
     2281 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct
     2341 ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta
     2401 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt
     2461 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct
     2521 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt
     2581 taatcactat accaattgag atgggctagt caatgataat tactagtcct tttcctttga
     2641 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga
     2701 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata
     2761 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat
     2821 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct
     2881 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc
     2941 aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg
     3001 tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag
     3061 gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg
     3121 cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca
     3181 gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat
     3241 tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta cccgtcttac
     3301 tgtccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     3361 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     3421 tctcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     3481 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     3541 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     3601 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     3661 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     3721 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     3781 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     3841 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     3901 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     3961 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     4021 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     4081 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     4141 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     4201 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     4261 ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     4321 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccgacgtcta
     4381 agaaaccatt a
//
