LOCUS       sequence-200463-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding coVenNB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-coVenNB
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     46..64
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1003..1089
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1627..2577)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2625..2847
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3339..3433)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3464..4258)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3575..3594)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4185..4204
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tccgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
       61 aggccctttc gtcttcacca cagctaacac cacgtcgtcc ctatctgctg ccctaggtct
      121 atgagtggtt gctggataac tttacgggca tgcataaggc tcgtatgata tattcaggga
      181 gaccacaacg gtttccctct acaaataatt ttgtttaact tttctagatt taagaaggag
      241 atatacatat gagcaaaggt gaagaactgt tcaccggcgt tgtgccaatt ctggttgagc
      301 tggatggtga cgtgaatggc cacaaatttt ccgtgtctgg tgaaggcgag ggtgatgcta
      361 cttatggcaa actgactctg aaactgatct gtaccaccgg caaactgcct gttccgtggc
      421 caactctggt cactactctg ggttacggcg tgcagtgttt tgcgcgttac ccggatcaca
      481 tgaaacagca tgacttcttc aaatctgcca tgccggaagg ctatgtccaa gaacgtacga
      541 tctttttcaa ggacgacggc aactataaaa cccgtgccga agttaaattc gagggtgaca
      601 ccctggttaa ccgcatcgaa ctgaaaggca ttgacttcaa agaggacggc aacattctgg
      661 gtcacaagct ggaatacaac tacaactccc acaacgttta cattactgct gacaagcaga
      721 aaaacggcat caaagcaaac ttcaagatcc gtcacaacat tgaagatggt ggcgtacagc
      781 tggcagatca ctaccagcag aacactccaa tcggtgatgg cccagtactg ctgccagata
      841 accattacct gtcctaccag agcaaactgt ctaaagaccc gaacgaaaaa cgtgaccaca
      901 tggtactgct ggaatttgtt accgcggcag gcattaccca cggtatggac gaactgtata
      961 aataaatgtc cagacctgca ggcatgcaag ctctagaggc atcaaataaa acgaaaggct
     1021 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     1081 aggacaaatc cgccgcccta gacctagggt acgggttttg ctgcccgcaa acgggctgtt
     1141 ctggtgttgc tagtttgtta tcagaatcgc agatccggct tcagccggtt tgccggctga
     1201 aagcgctatt tcttccagaa ttgccatgat tttttcccca cgggaggcgt cactggctcc
     1261 cgtgttgtcg gcagctttga ttcgataagc agcatcgcct gtttcaggct gtctatgtgt
     1321 gactgttgag ctgtaacaag ttgtctcagg tgttcaattt catgttctag ttgctttgtt
     1381 ttactggttt cacctgttct attaggtgtt acatgctgtt catctgttac attgtcgatc
     1441 tgttcatggt gaacagcttt gaatgcacca aaaactcgta aaagctctga tgtatctatc
     1501 ttttttacac cgttttcatc tgtgcatatg gacagttttc cctttgatat gtaacggtga
     1561 acagttgttc tacttttgtt tgttagtctt gatgcttcac tgatagatac aagagccata
     1621 agaacctcag atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg
     1681 cgtgagccat gagaacgaac cattgagatc atacttactt tgcatgtcac tcaaaaattt
     1741 tgcctcaaaa ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag
     1801 tccgttatgt aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt
     1861 tatctggttg ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa
     1921 aatcaacgta tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt
     1981 gtttaaatct ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta
     2041 gttattttca agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt
     2101 gtgagttttc ttttgtgtta gttcttttaa taaccactca taaatcctca tagagtattt
     2161 gttttcaaaa gacttaacat gttccagatt atattttatg aattttttta actggaaaag
     2221 ataaggcaat atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata
     2281 gtttgtccac tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct
     2341 cgtcatcagc tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt
     2401 catcatctga gcgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt
     2461 ttcaatcgtg gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt
     2521 taagtcatag cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca
     2581 tctcaattgg tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata
     2641 attactagtc cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg
     2701 aaaacttgta aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt
     2761 tgtttatatt caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag
     2821 aatagatccc agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag
     2881 gatgtcgcaa acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag
     2941 tagcaccctc gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc
     3001 acctgagtcg ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga
     3061 atgggggtaa atggcactac aggcgccttt tatggattca tgcaaggaaa ctacccataa
     3121 tacaagaaaa gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg
     3181 ctatctgact ttttgctgtt cagcagttcc tgccctctga ttttccagtc tgaccacttc
     3241 ggattatccc gtgacaggtc attcagactg gctaatgcac ccagtaaggc agcggtatca
     3301 tcaacaggct tacccgtctt actgtcccta gtgcttggat tctcaccaat aaaaaacgcc
     3361 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     3421 atcaacagga gtccaagcga gctctcgaac cccagagtcc cgctcagaag aactcgtcaa
     3481 gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga
     3541 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt
     3601 cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat
     3661 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt
     3721 cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt
     3781 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc
     3841 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca
     3901 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct
     3961 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca
     4021 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca
     4081 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg
     4141 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga
     4201 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc
     4261 gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat cagatccttg
     4321 gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc
     4381 cagctggcaa t
//
