LOCUS       sequence-200465-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding comCerulean3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-comCerulean3
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..78
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1017..1103
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1641..2591)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2639..2861
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3353..3447)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3478..4272)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3589..3608)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4199..4218
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccccagctgg caattccgac gtctaagaaa ccattattat catgacatta acctataaaa
       61 ataggcgtat cacgaggccc tttcgtcttc accacagcta acaccacgtc gtccctatct
      121 gctgccctag gtctatgagt ggttgctgga taactttacg ggcatgcata aggctcgtat
      181 gatatattca gggagaccac aacggtttcc ctctacaaat aattttgttt aacttttcta
      241 gatttaagaa ggagatatac atatgagcaa gggcgaggag ctgtttactg gtgtggttcc
      301 tatcctggtc gaactggacg gtgatgtcaa cggtcataag ttcagcgtta gcggcgaggg
      361 tgaaggcgac gcaacctacg gtaagctgac cctgaagttc atttgtacga cgggtaagct
      421 gccggtgcct tggcctaccc tggttaccac cctgagttgg ggtgttcaat gcttcgctcg
      481 ctatccagac cacatgaagc agcacgattt ttttaagagc gccatgccag agggttacgt
      541 tcaggagcgc accattttct ttaaagatga tggtaattac aagactcgcg cagaggtgaa
      601 gtttgaaggc gacactctgg tgaatcgtat tgagctgaag ggcatcgatt ttaaggaaga
      661 tggtaatatc ctgggccata aactggaata taatgcgatt cacggtaatg tgtatatcac
      721 cgccgataaa caaaagaatg gtattaaggc gaattttgga ctgaactgta atatcgagga
      781 cggctctgtg caactggctg accattatca acaaaatacc ccgattggcg acggtcctgt
      841 tctgctgccg gacaatcact atctgagcac tcaatctaag ctgagcaaag atcctaatga
      901 gaagcgcgat cacatggtgc tgctggaatt tgtaacggct gctggcatca cgcatggcat
      961 ggatgagctg tataaataaa tgtccagacc tgcaggcatg caagctctag aggcatcaaa
     1021 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1081 acgctctcct gagtaggaca aatccgccgc cctagaccta gggtacgggt tttgctgccc
     1141 gcaaacgggc tgttctggtg ttgctagttt gttatcagaa tcgcagatcc ggcttcagcc
     1201 ggtttgccgg ctgaaagcgc tatttcttcc agaattgcca tgattttttc cccacgggag
     1261 gcgtcactgg ctcccgtgtt gtcggcagct ttgattcgat aagcagcatc gcctgtttca
     1321 ggctgtctat gtgtgactgt tgagctgtaa caagttgtct caggtgttca atttcatgtt
     1381 ctagttgctt tgttttactg gtttcacctg ttctattagg tgttacatgc tgttcatctg
     1441 ttacattgtc gatctgttca tggtgaacag ctttgaatgc accaaaaact cgtaaaagct
     1501 ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt tttccctttg
     1561 atatgtaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct tcactgatag
     1621 atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg
     1681 gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatactt actttgcatg
     1741 tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa agcatcgtgt
     1801 agtgtttttc ttagtccgtt atgtaggtag gaatctgatg taatggttgt tggtattttg
     1861 tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat ttgtctatct
     1921 agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac caccaatttc
     1981 atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg gttaagcctt
     2041 ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc aaggctaatc
     2101 tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca ctcataaatc
     2161 ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt tatgaatttt
     2221 tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa tttttcgctt
     2281 gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa aggattcctg
     2341 atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc ataagcattt
     2401 tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac cgtccgttct
     2461 ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa aattagcttg
     2521 gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt gaaaacaact
     2581 aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca attgagatgg
     2641 gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg taaattctgc
     2701 tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc cgctagacct
     2761 ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa gaaagaataa
     2821 aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta gtcagttccg
     2881 cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag accttaaaac
     2941 cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc
     3001 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac
     3061 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag
     3121 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg
     3181 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc
     3241 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta
     3301 aggcagcggt atcatcaaca ggcttacccg tcttactgtc cctagtgctt ggattctcac
     3361 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     3421 tcattactgg atctatcaac aggagtccaa gcgagctctc gaaccccaga gtcccgctca
     3481 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     3541 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     3601 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     3661 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     3721 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     3781 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     3841 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     3901 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     3961 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     4021 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     4081 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     4141 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     4201 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     4261 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg
     4321 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     4381 accagagggc g
//
