LOCUS       sequence-200467-        4391 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding coSCFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB1-coSCFP1
FEATURES             Location/Qualifiers
     source          1..4391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..46
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      985..1071
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1609..2559)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2607..2829
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3321..3415)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3446..4240)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3557..3576)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4167..4186
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac
       61 cacagctaac accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata
      121 actttacggg catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct
      181 ctacaaataa ttttgtttaa cttttctaga tttaagaagg agatatacat atgagcaagg
      241 gcgaggagct gtttactggt gtggttccta tcctggtcga actggacggt gatgtcaacg
      301 gtcataagtt cagcgttagc ggcgagggtg aaggcgacgc aacctacggt aagctgaccc
      361 tgaagttcat ttgtacgacg ggtaagctgc cggtgccttg gcctaccctg gttaccaccc
      421 tgacctgggg tctgcaatgc ttcgctcgct atccagacca catgaagcag cacgattttt
      481 ttaagagcgc catgccagag ggttacgttc aggagcgcac cattttcttt aaagatgatg
      541 gtaattacaa gactcgcgca gaggtgaagt ttgaaggcga cactctggtg aatcgtattg
      601 agctgaaggg catcgatttt aaggaagatg gtaatatcct gggccataaa ctggaatata
      661 attacattag ccataatgtg tatatcaccg ccgataaaca aaagaatggt attaaggcga
      721 attttaaaat tcgccataat atcgaggacg gcggcgtgca actggctgac cattatcaac
      781 aaaatacccc gattggcgac ggtcctgttc tgctgccgga caatcactat ctgagcactc
      841 aatctaagct gagcaaagat cctaatgaga agcgcgatca catggtgctg ctggaatttg
      901 taacggctgc tggcatcacg catggcatgg atgagctgta taaataaatg tccagacctg
      961 caggcatgca agctctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1021 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
     1081 tagacctagg gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt
     1141 tatcagaatc gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag
     1201 aattgccatg attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt
     1261 gattcgataa gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca
     1321 agttgtctca ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt
     1381 ctattaggtg ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct
     1441 ttgaatgcac caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca
     1501 tctgtgcata tggacagttt tccctttgat atgtaacggt gaacagttgt tctacttttg
     1561 tttgttagtc ttgatgcttc actgatagat acaagagcca taagaacctc agatccttcc
     1621 gtatttagcc agtatgttct ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga
     1681 accattgaga tcatacttac tttgcatgtc actcaaaaat tttgcctcaa aactggtgag
     1741 ctgaattttt gcagttaaag catcgtgtag tgtttttctt agtccgttat gtaggtagga
     1801 atctgatgta atggttgttg gtattttgtc accattcatt tttatctggt tgttctcaag
     1861 ttcggttacg agatccattt gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg
     1921 gcggcctcgc ttatcaacca ccaatttcat attgctgtaa gtgtttaaat ctttacttat
     1981 tggtttcaaa acccattggt taagcctttt aaactcatgg tagttatttt caagcattaa
     2041 catgaactta aattcatcaa ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt
     2101 tagttctttt aataaccact cataaatcct catagagtat ttgttttcaa aagacttaac
     2161 atgttccaga ttatatttta tgaatttttt taactggaaa agataaggca atatctcttc
     2221 actaaaaact aattctaatt tttcgcttga gaacttggca tagtttgtcc actggaaaat
     2281 ctcaaagcct ttaaccaaag gattcctgat ttccacagtt ctcgtcatca gctctctggt
     2341 tgctttagct aatacaccat aagcattttc cctactgatg ttcatcatct gagcgtattg
     2401 gttataagtg aacgataccg tccgttcttt ccttgtaggg ttttcaatcg tggggttgag
     2461 tagtgccaca cagcataaaa ttagcttggt ttcatgctcc gttaagtcat agcgactaat
     2521 cgctagttca tttgctttga aaacaactaa ttcagacata catctcaatt ggtctaggtg
     2581 attttaatca ctataccaat tgagatgggc tagtcaatga taattactag tccttttcct
     2641 ttgagttgtg ggtatctgta aattctgcta gacctttgct ggaaaacttg taaattctgc
     2701 tagaccctct gtaaattccg ctagaccttt gtgtgttttt tttgtttata ttcaagtggt
     2761 tataatttat agaataaaga aagaataaaa aaagataaaa agaatagatc ccagccctgt
     2821 gtataactca ctactttagt cagttccgca gtattacaaa aggatgtcgc aaacgctgtt
     2881 tgctcctcta caaaacagac cttaaaaccc taaaggctta agtagcaccc tcgcaagctc
     2941 gggcaaatcg ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt
     3001 ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact
     3061 acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca
     3121 cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg
     3181 ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg
     3241 tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc
     3301 ttactgtccc tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt
     3361 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc
     3421 gagctctcga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     3481 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     3541 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     3601 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     3661 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     3721 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     3781 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     3841 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     3901 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     3961 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     4021 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg
     4081 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     4141 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     4201 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     4261 tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc
     4321 agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg
     4381 tctaagaaac c
//
