LOCUS       sequence-200471-        9562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and
            Raf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Duan L, Hope J, Ong Q, Lou HY, Kim N, McCarthy C, Acero V, Lin MZ, 
            Cui B
  TITLE     Understanding CRY2 interactions for optical control of intracellular
            signaling.
  JOURNAL   Nat Commun. 2017 Sep 15;8(1):547. doi: 10.1038/s41467-017-00648-8.
  PUBMED    28916751
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-15"; volume: "8(1):547. doi"; pages: " 
            10.1038/s41467-017-00648-8"
COMMENT     Sequence Label: CRY2high-mCherry-Raf1
FEATURES             Location/Qualifiers
     source          1..9562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(32..51)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        223..602
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        603..806
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     757..777
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             863..2356
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRRAQIMIGAA"
     CDS             2399..3106
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2544..2562)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2812..2831
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5103..5121)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5104..5121)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5141..5158)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5147..5371
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5417..5845
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5504..5523)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5714..5735
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5854..5874)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5859..6188
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6039..6174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6101..6120
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6255..7049
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6309..6328)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6919..6938
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7223..7344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7260..7279)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7314..7333
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7393..7409)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7393..7409)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7406..7428)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7417..7433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7441..7447,7448..7465,7466..7471))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7486..7507
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7624..7641)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7795..8383)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7875..7894)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8554..9345,9346..9414))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9177..9196
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9415..9519)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
       61 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
      121 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
      181 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
      241 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      301 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      361 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      421 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      481 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctgacattat gcccagtaca
      541 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      601 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      661 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      721 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      781 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      841 ggactcagat ctcgaggcca ccatgaagat ggacaaaaag actatagttt ggtttagaag
      901 agacctaagg attgaggata atcctgcatt agcagcagct gctcacgaag gatctgtttt
      961 tcctgtcttc atttggtgtc ctgaagaaga aggacagttt tatcctggaa gagcttcaag
     1021 atggtggatg aaacaatcac ttgctcactt atctcaatcc ttgaaggctc ttggatctga
     1081 cctcacttta atcaaaaccc acaacacgat ttcagcgatc ttggattgta tccgcgttac
     1141 cggtgctaca aaagtcgtct ttaaccacct ctatgatcct gtttcgttag ttcgggacca
     1201 taccgtaaag gagaagctgg tggaacgtgg gatctctgtg caaagctaca atggagatct
     1261 attgtatgaa ccgtgggaga tatactgcga aaagggcaaa ccttttacga gtttcaattc
     1321 ttactggaag aaatgcttag atatgtcgat tgaatccgtt atgcttcctc ctccttggcg
     1381 gttgatgcca ataactgcag cggctgaagc gatttgggcg tgttcgattg aagaactagg
     1441 gctggagaat gaggccgaga aaccgagcaa tgcgttgtta actagagctt ggtctccagg
     1501 atggagcaat gctgataagt tactaaatga gttcatcgag aagcagttga tagattatgc
     1561 aaagaacagc aagaaagttg ttgggaattc tacttcacta ctttctccgt atctccattt
     1621 cggggaaata agcgtcagac acgttttcca gtgtgcccgg atgaaacaaa ttatatgggc
     1681 aagagataag aacagtgaag gagaagaaag tgcagatctt tttcttaggg gaatcggttt
     1741 aagagagtat tctcggtata tatgtttcaa cttcccgttt actcacgagc aatcgttgtt
     1801 gagtcatctt cggtttttcc cttgggatgc tgatgttgat aagttcaagg cctggagaca
     1861 aggcaggacc ggttatccgt tggtggatgc cggaatgaga gagctttggg ctaccggatg
     1921 gatgcataac agaataagag tgattgtttc aagctttgct gtgaagtttc ttctccttcc
     1981 atggaaatgg ggaatgaagt atttctggga tacacttttg gatgctgatt tggaatgtga
     2041 catccttggc tggcagtata tctctgggag tatccccgat ggccacgagc ttgatcgctt
     2101 ggacaatccc gcgttacaag gcgccaaata tgacccagaa ggtgagtaca taaggcaatg
     2161 gcttcccgag cttgcgagat tgccaactga atggatccat catccatggg acgctccttt
     2221 aaccgtactc aaagcttctg gtgtggaact cggaacaaac tatgcgaaac ccattgtaga
     2281 catcgacaca gctcgtgagc tactagctaa agctatttca agaacccgta gagcacagat
     2341 catgatcgga gcagcagaat tcgatagtgc tggtagtgct ggtagtgctg gtactagtat
     2401 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     2461 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     2521 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     2581 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     2641 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     2701 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     2761 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     2821 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     2881 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     2941 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     3001 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     3061 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtccg gactcagatc
     3121 tcgagtgatg gagcacatac agggagcttg gaagacgatc agcaatggtt ttggattcaa
     3181 agatgccgtg tttgatggct ccagctgcat ctctcctaca atagttcagc agtttggcta
     3241 tcagcgccgg gcatcagatg atggcaaact cacagatcct tctaagacaa gcaacactat
     3301 ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat gtgcgaaatg gaatgagctt
     3361 gcatgactgc cttatgaaag cactcaaggt gaggggcctg caaccagagt gctgtgcagt
     3421 gttcagactt ctccacgaac acaaaggtaa aaaagcacgc ttagattgga atactgatgc
     3481 tgcgtctttg attggagaag aacttcaagt agatttcctg gatcatgttc ccctcacaac
     3541 acacaacttt gctcggaaga cgttcctgaa gcttgccttc tgtgacatct gtcagaaatt
     3601 cctgctcaat ggatttcgat gtcagacttg tggctacaaa tttcatgagc actgtagcac
     3661 caaagtacct actatgtgtg tggactggag taacatcaga caactcttat tgtttccaaa
     3721 ttccactatt ggtgatagtg gagtcccagc actaccttct ttgactatgc gtcgtatgcg
     3781 agagtctgtt tccaggatgc ctgttagttc tcagcacaga tattctacac ctcacgcctt
     3841 cacctttaac acctccagtc cctcatctga aggttccctc tcccagaggc agaggtcgac
     3901 atccacacct aatgtccaca tggtcagcac caccctgcct gtggacagca ggatgattga
     3961 ggatgcaatt cgaagtcaca gcgaatcagc ctcaccttca gccctgtcca gtagccccaa
     4021 caatctgagc ccaacaggct ggtcacagcc gaaaaccccc gtgccagcac aaagagagcg
     4081 ggcaccagta tctgggaccc aggagaaaaa caaaattagg cctcgtggac agagagattc
     4141 aagctattat tgggaaatag aagccagtga agtgatgctg tccactcgga ttgggtcagg
     4201 ctcttttgga actgtttata agggtaaatg gcacggagat gttgcagtaa agatcctaaa
     4261 ggttgtcgac ccaaccccag agcaattcca ggccttcagg aatgaggtgg ctgttctgcg
     4321 caaaacacgg catgtgaaca ttctgctttt catggggtac atgacaaagg acaacctggc
     4381 aattgtgacc cagtggtgcg agggcagcag cctctacaaa cacctgcatg tccaggagac
     4441 caagtttcag atgttccagc taattgacat tgcccggcag acggctcagg gaatggacta
     4501 tttgcatgca aagaacatca tccatagaga catgaaatcc aacaatatat ttctccatga
     4561 aggcttaaca gtgaaaattg gagattttgg tttggcaaca gtaaagtcac gctggagtgg
     4621 ttctcagcag gttgaacaac ctactggctc tgtcctctgg atggccccag aggtgatccg
     4681 aatgcaggat aacaacccat tcagtttcca gtcggatgtc tactcctatg gcatcgtatt
     4741 gtatgaactg atgacggggg agcttcctta ttctcacatc aacaaccgag atcagatcat
     4801 cttcatggtg ggccgaggat atgcctcccc agatcttagt aagctatata agaactgccc
     4861 caaagcaatg aagaggctgg tagctgactg tgtgaagaaa gtaaaggagg agaggcctct
     4921 ttttccccag atcctgtctt ccattgagct gctccaacac tctctaccga agatcaaccg
     4981 gagcgcttcc gagccatcct tgcatcgggc agcccacact gaggatatca atgcttgcac
     5041 gctgaccacg tccccgaggc tgcctgtctt ctaagcggcc gctcgagtct agagggccct
     5101 attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt gccttctagt
     5161 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     5221 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     5281 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     5341 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     5401 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5461 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5521 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5581 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5641 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5701 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5761 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5821 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     5881 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5941 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6001 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6061 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     6121 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     6181 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     6241 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6301 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6361 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6421 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6481 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6541 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     6601 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     6661 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6721 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     6781 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     6841 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     6901 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     6961 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     7021 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     7081 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     7141 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     7201 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     7261 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7321 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     7381 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7441 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7501 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7561 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     7621 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     7681 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     7741 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     7801 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     7861 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     7921 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     7981 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     8041 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     8101 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     8161 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     8221 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     8281 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     8341 tgtttgcaag cagcagatta cgtgcagaaa aaaaggatct caagaagatc ctttgatctt
     8401 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     8461 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     8521 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     8581 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     8641 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     8701 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     8761 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     8821 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     8881 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     8941 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9001 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9061 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9121 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9181 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9241 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9301 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9361 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     9421 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     9481 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     9541 acctgacgtc gacggatcgg ga
//
