LOCUS       sequence-200482-        9706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9 plasmid expressing 3A sRNA trigger.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Galizi R, Duncan JN, Rostain W, Quinn CM, Storch M, Kushwaha M, 
            Jaramillo A
  TITLE     Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and
            Diagnostics.
  JOURNAL   CRISPR J. 2020 Oct;3(5):398-408. doi: 10.1089/crispr.2020.0029.
  PUBMED    33095053
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2020"; journalName: "CRISPR J"; date: "2020-10"; 
            volume: "3"; issue: "5"; pages: "398-408"
COMMENT     Sequence Label: PAJ390
FEATURES             Location/Qualifiers
     source          1..9706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1708..2367)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2282..2301
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2368..2470)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    2855..2873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2880..2898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(3242..3259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3376..3921)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(3456..3475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(4033..4038,4039..4055,4056..4061)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     misc_RNA        complement(4379..4457)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             join(4753..4779,4780..4782,4783..7269,7270..7272,
                     7273..8859)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    8880..9011
                     /label=crRNA leader
                     /note="crRNA leader sequence for the Streptococcus pyogenes
                     CRISPR/Cas system"
     repeat_region   9012..9047
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   9078..9113
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     primer_bind     complement(9542..9561)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc
       61 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
      121 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
      181 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
      241 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
      301 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
      361 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
      421 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
      481 agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc attggaccgc
      541 tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
      601 taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
      661 ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
      721 tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
      781 atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
      841 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
      901 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
      961 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     1021 aagtctggaa acgcggaagt cccctacgtg ctgctgaagt tgcccgcaac agagagtgga
     1081 accaaccggt gataccacga tactatgact gagagtcaac gccatgagcg gcctcatttc
     1141 ttattctgag ttacaacagt ccgcaccgct gccggtagct ccttccggtg ggcgcggggc
     1201 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     1261 ccggcagcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
     1321 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga
     1381 acacctacat ctgtattaac gaagcgctaa ccgtttttat caggctctgg gaggcagaat
     1441 aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg
     1501 catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat
     1561 agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt
     1621 cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcaac caggcgttta
     1681 agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtactg
     1741 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     1801 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     1861 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     1921 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     1981 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2041 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2101 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg
     2161 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2221 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2281 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2341 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2401 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2461 gtgccgatca acgtctcatt ttcgccaaaa gttggcccgg ggcttcccgg tatcaacagg
     2521 gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcggcgca
     2581 aagtgcgtcg ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg
     2641 ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac agcttgattc
     2701 tagctgatcg tggaccggaa ggtgagccag tgagttgatt gcagtccagt tacgctggag
     2761 tctgaggctc gtcctgaatg atatgcgacc gccggagggt tgcgtttgag acgggcgaca
     2821 gatcgacact gctcgatccg ctcgcaccga attctcccta tcagtgatag agattgacat
     2881 ccctatcagt gatagagata ctgagcacgg cagacaaaga aacctacgtt gaacagcatg
     2941 aagtcgcatc cttagcgaaa gctaaggatt ttttttctgc agggatcgac gagagcagcg
     3001 cgactggatc agttctggac gagcgagctg tcgtccgacc cgtgatctta cggcattata
     3061 cgtatgatcg ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag
     3121 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     3181 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     3241 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     3301 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     3361 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     3421 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3481 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     3541 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     3601 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     3661 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     3721 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     3781 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
     3841 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
     3901 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt
     3961 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
     4021 taattctaat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
     4081 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
     4141 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
     4201 cttgcgggat tacgaaatca tcctgtggag cttagtaggt ttagcaagat ggcagcgcct
     4261 aaatgtagaa tgataaaagg attaagagat taatttccct aaaaatgata aaacaagcgt
     4321 tttgaaagcg cttgtttttt tggtttgcag tcagagtaga atagaagtat caaaaaaagc
     4381 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatg
     4441 ctgttttgaa tggttccaac aagattattt tataactttt ataacaaata atcaaggaga
     4501 aattcaaaga aatttatcag ccataaaaca atacttaata ctatagaatg ataacaaaat
     4561 aaactacttt ttaaaagaat tttgtgttat aatctattta ttattaagta ttgggtaata
     4621 ttttttgaag agatattttg aaaaagaaaa attaaagcat attaaactaa tttcggaggt
     4681 cattaaaact attattgaaa tcatcaaact cattatggat ttaatttaaa ctttttattt
     4741 taggaggcaa aaatggataa gaaatactca ataggcttag ctatcggcac aaatagcgtc
     4801 ggatgggcgg tgatcactga tgaatataag gttccgtcta aaaagttcaa ggttctggga
     4861 aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt tgacagtgga
     4921 gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac acgtcggaag
     4981 aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt agatgatagt
     5041 ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca tgaacgtcat
     5101 cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc aactatctat
     5161 catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt aatctatttg
     5221 gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga tttaaatcct
     5281 gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa tcaattattt
     5341 gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc tgcacgattg
     5401 agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa gaaaaatggc
     5461 ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa atcaaatttt
     5521 gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga tgatttagat
     5581 aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc taagaattta
     5641 tcagatgcta ttttactttc agatatccta agagtaaata ctgaaataac taaggctccc
     5701 ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac tcttttaaaa
     5761 gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga tcaatcaaaa
     5821 aacggatatg caggttatat tgatggggga gctagccaag aagaatttta taaatttatc
     5881 aaaccaattt tagaaaaaat ggatggtact gaggaattat tggtgaaact aaatcgtgaa
     5941 gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca aattcacttg
     6001 ggtgagctgc atgctatttt gagaagacaa gaagactttt atccattttt aaaagacaat
     6061 cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg tccattggcg
     6121 cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat taccccatgg
     6181 aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga acgcatgaca
     6241 aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt gctttatgag
     6301 tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaagg aatgcgaaaa
     6361 ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt caaaacaaat
     6421 cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga atgttttgat
     6481 agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac ctaccatgat
     6541 ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga agatatctta
     6601 gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga ggaaagactt
     6661 aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg tcgccgttat
     6721 actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa gcaatctggc
     6781 aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt tatgcagctg
     6841 atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt gtctggacaa
     6901 ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat taaaaaaggt
     6961 attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg gcataagcca
     7021 gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg ccagaaaaat
     7081 tcgcgagagc gtatgaaacg aatcgaagaa ggtatcaaag aattaggaag tcagattctt
     7141 aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct ctattatctc
     7201 caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt aagtgattat
     7261 gatgtcgatg ccattgttcc acaaagtttc cttaaagacg attcaataga caataaggtc
     7321 ttaacgcgtt ctgataaaaa tcgtggtaaa tcggataacg ttccaagtga agaagtagtc
     7381 aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac tcaacgtaag
     7441 tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa agctggtttt
     7501 atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca aattttggat
     7561 agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt taaagtgatt
     7621 accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta taaagtacgt
     7681 gagattaaca attaccatca tgcccatgat gcgtatctaa atgccgtcgt tggaactgct
     7741 ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta taaagtttat
     7801 gatgttcgta aaatgattgc taagtctgag caagaaatag gcaaagcaac cgcaaaatat
     7861 ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc aaatggagag
     7921 attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt ctgggataaa
     7981 gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa tattgtcaag
     8041 aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa aagaaattcg
     8101 gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg ttttgatagt
     8161 ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa atcgaagaag
     8221 ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc ctttgaaaaa
     8281 aatccgattg actttttaga agctaaagga tataaggaag ttaaaaaaga cttaatcatt
     8341 aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat gctggctagt
     8401 gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt gaatttttta
     8461 tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga acaaaaacaa
     8521 ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat cagtgaattt
     8581 tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc atataacaaa
     8641 catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt tacgttgacg
     8701 aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg taaacgatat
     8761 acgtctacaa aagaagtttt agatgccact cttatccatc aatccatcac tggtctttat
     8821 gaaacacgca ttgatttgag tcagctagga ggtgactgaa gtatatttta gatgaagatt
     8881 atttcttaat aactaaaaat atggtataat actcttaata aatgcagtaa tacaggggct
     8941 tttcaagact gaagtctagc tgagacaaat agtgcgatta cgaaattttt tagacaaaaa
     9001 tagtctacga ggttttagag ctatgctgtt ttgaatggtc ccaaaactga gaccagtctc
     9061 ggaagctcaa aggtctcgtt ttagagctat gctgttttga atggtcccaa aacttcagca
     9121 cactgagact tgttgagttc catgttttag agctatgctg ttttgaatgg actccattca
     9181 acattgccga tgataacttg agaaagaggg ttaataccag cagtcggata ccttcctatt
     9241 ctttctgtta aagcgttttc atgttataat aggcaaaaga agagtagtgt gatcgtccat
     9301 tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt
     9361 ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc
     9421 gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg
     9481 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     9541 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     9601 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     9661 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctca
//
