LOCUS       sequence-20049-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Hoppe AD, Swanson JA
  TITLE     Cdc42, Rac1, and Rac2 display distinct patterns of activation during
            phagocytosis.
  JOURNAL   Mol Biol Cell 2004 Aug;15(8):3509-19. Epub 2004 May 28.
  PUBMED    15169870
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell"; date: "2004-08"; volume: "15(8)"; pages: "3509-19. Epub 2004 
            May 28"
COMMENT     Sequence Label: YFP-Rac1(N17)
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(15..62,63..65,66..593)
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     CDS             15..593
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     polyA_signal    762..883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(799..818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     853..872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccggactcag atctatgcag gccatcaagt gtgtggtggt gggagacgga gctgtaggta
       61 aaaattgcct actgatcagt tacacaacca atgcatttcc tggagaatat atccctactg
      121 tctttgacaa ttattctgcc aatgttatgg tagatggaaa accggtgaat ctgggcttat
      181 gggatacagc tggacaagaa gattatgaca gattacgccc cctatcctat ccgcaaacag
      241 atgtgttctt aatttgcttt tcccttgtga gtcctgcatc atttgaaaat gtccgtgcaa
      301 agtggtatcc tgaggtgcgg caccactgtc ccaacactcc catcatccta gtgggaacta
      361 aacttgatct tagggatgat aaagacacga tcgagaaact gaaggagaag aagctgactc
      421 ccatcaccta tccgcagggt ctagccatgg ctaaggagat tggtgctgta aaatacctgg
      481 agtgctcggc gctcacacag cgaggcctca agacagtgtt tgacgaagcg atccgagcag
      541 tcctctgccc gcctcccgtg aagaagagga agagaaaatg cctgctgttg tgacaagctt
      601 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca
      661 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
      721 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      781 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      841 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta atttgtaagc
      901 gttaatattt tgttaaaatt cgcgttaaat tttttgttaa tcagctcatt ttttaaccaa
      961 taggccgaaa tcggcaaaa
//
