LOCUS       sequence-200500-        1239 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for red fluorescent Ca2+ indicator 
            K-GECO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Shen Y, Dana H, Abdelfattah AS, Patel R, Shea J, Molina RS, Rawal B,
            Rancic V, Chang YF, Wu L, Chen Y, Qian Y, Wiens MD, Hambleton N, 
            Ballanyi K, Hughes TE, Drobizhev M, Kim DS, Koyama M, Schreiter ER, 
            Campbell RE
  TITLE     A genetically encoded Ca(2+) indicator based on circularly 
            permutated sea anemone red fluorescent protein eqFP578.
  JOURNAL   BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1186/s12915-018-0480-0.
  PUBMED    29338710
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2018-01-16"; volume: "16(1):9. doi"; pages: " 
            10.1186/s12915-018-0480-0"
COMMENT     Sequence Label: pBAD-K-GECO1
FEATURES             Location/Qualifiers
     source          1..1239
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggcagcg tgaagctgat ccccagcctg accaccgtga tcctcgtgaa gtccatgctg
       61 cggaagcgga gcttcggcaa ccccttcaag tataatacgg agaccctgta ccccgctgac
      121 ggcggcctgg aaggcgcatg tgacatggcc ctgaagctcg tgggcggggg ccacctgaac
      181 tgcagccttg agaccacata cagatccaag aaacccgcta cgaacctcaa gatgcccggt
      241 gtctacaacg tggaccacag actggaacga atcaaagagg ccgacgatga gacctacgtc
      301 gagctgcacg aggtggctgt ggccagatac gtgggcctgg gtggtggcgg aggtacaggc
      361 gggagtgtga gcgagctgat taaggagaac atgccaatga agctgtacat ggagggcacc
      421 gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
      481 cagaccatga gaatcaaggt cgtcgagggc ggccctctcc ccttcgcctt cgacatcctg
      541 gctaccagct tcatgtacgg cagcagaacc ttcatcaagc accctcctgg catccccgac
      601 ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
      661 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
      721 gtcaaggtta gagggatgaa cttcccagcc aacggccctg tgatgcagaa gaaaacactc
      781 ggctgggagg ccagtaatgg ccaactgact gaagagcaga tcgcagaatt taaagaggct
      841 ttctccctat ttgacaagga cggggatggg acgataacaa ccaaggagct ggggacggtg
      901 atgcggtctc tggggcagaa ccccacagaa gcggagctgc aggacatgat caatgaagta
      961 gatgccgacg gtgacggcac attcgacttc cctgagttcc tgacgatgat ggcaagaaaa
     1021 atgagttata gagtcactga agaggaaatt agagaagcgt tccgcgtgtt tgataaggac
     1081 ggcaatggct acatcggcgc agcagagctt cgccacgtga tgacagacct tggagagaag
     1141 ttaacagatg aggaggttga tgaaatgatc agggtagcag acatcgatgg ggatggtcag
     1201 gtaaactacg aagagtttgt acaaatgatg acagcgaag
//
