LOCUS       sequence-200503-        6708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6708)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 6708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-LaminA-C-18
FEATURES             Location/Qualifiers
     source          1..6708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1260
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(698..716)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     966..985
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3436..3557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3473..3492)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3527..3546
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3564..4019)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3701..3722)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3913..3932
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4046..4150
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4152..4509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4152..4343
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4175..4195)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4360..4495
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4544..5338
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4544..5338
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4598..4617)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5208..5227
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5526..5545)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5570..5617
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5946..6534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6435..6454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1261 tccggactca gatctggcag cggtggaggc agcgcatccg gcggaagcgg aagcgagacc
     1321 ccgtcccagc ggcgcgccac ccgcagcggg gcgcaggcca gctccactcc gctgtcgccc
     1381 acccgcatca cccggctgca ggagaaggag gacctgcagg agctcaatga tcgcttggcg
     1441 gtctacatcg accgtgtgcg ctcgctggaa acggagaacg cagggctgcg ccttcgcatc
     1501 accgagtctg aagaggtggt cagccgcgag gtgtccggca tcaaggccgc ctacgaggcc
     1561 gagctcgggg atgcccgcaa gacccttgac tcagtagcca aggagcgcgc ccgcctgcag
     1621 ctggagctga gcaaagtgcg tgaggagttt aaggagctga aagcgcgcaa taccaagaag
     1681 gagggtgacc tgatagctgc tcaggctcgg ctgaaggacc tggaggctct gctgaactcc
     1741 aaggaggccg cactgagcac tgctctcagt gagaagcgca cgctggaggg cgagctgcat
     1801 gatctgcggg gccaggtggc caagcttgag gcagccctag gtgaggccaa gaagcaactt
     1861 caggatgaga tgctgcggcg ggtggatgct gagaacaggc tgcagaccat gaaggaggaa
     1921 ctggacttcc agaagaacat ctacagtgag gagctgcgtg agaccaagcg ccgtcatgag
     1981 acccgactgg tggagattga caatgggaag cagcgtgagt ttgagagccg gctggcggat
     2041 gcgctgcagg aactgcgggc ccagcatgag gaccaggtgg agcagtataa gaaggagctg
     2101 gagaagactt attctgccaa gctggacaat gccaggcagt ctgctgagag gaacagcaac
     2161 ctggtggggg ctgcccacga ggagctgcag cagtcgcgca tccgcatcga cagcctctct
     2221 gcccagctca gccagctcca gaagcagctg gcagccaagg aggcgaagct tcgagacctg
     2281 gaggactcac tggcccgtga gcgggacacc agccggcggc tgctggcgga aaaggagcgg
     2341 gagatggccg agatgcgggc aaggatgcag cagcagctgg acgagtacca ggagcttctg
     2401 gacatcaagc tggccctgga catggagatc cacgcctacc gcaagctctt ggagggcgag
     2461 gaggagaggc tacgcctgtc ccccagccct acctcgcagc gcagccgtgg ccgtgcttcc
     2521 tctcactcat cccagacaca gggtgggggc agcgtcacca aaaagcgcaa actggagtcc
     2581 actgagagcc gcagcagctt ctcacagcac gcacgcacta gcgggcgcgt ggccgtggag
     2641 gaggtggatg aggagggcaa gtttgtccgg ctgcgcaaca agtccaatga ggaccagtcc
     2701 atgggcaatt ggcagatcaa gcgccagaat ggagatgatc ccttgctgac ttaccggttc
     2761 ccaccaaagt tcaccctgaa ggctgggcag gtggtgacga tctgggctgc aggagctggg
     2821 gccacccaca gcccccctac cgacctggtg tggaaggcac agaacacctg gggctgcggg
     2881 aacagcctgc gtacggctct catcaactcc actggggaag aagtggccat gcgcaagctg
     2941 gtgcgctcag tgactgtggt tgaggacgac gaggatgagg atggagatga cctgctccat
     3001 caccaccacg gctcccactg cagcagctcg ggggaccccg ctgagtacaa cctgcgctcg
     3061 cgcaccgtgc tgtgcgggac ctgcgggcag cctgccgaca aggcatctgc cagcggctca
     3121 ggagcccagg tgggcggacc catctcctct ggctcttctg cctccagtgt cacggtcact
     3181 cgcagctacc gcagtgtggg gggcagtggg ggtggcagct tcggggacaa tctggtcacc
     3241 cgctcctacc tcctgggcaa ctccagcccc cgaacccaga gcccccagaa ctgcagcatc
     3301 atgtaaggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     3361 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     3421 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3481 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3541 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     3601 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     3661 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     3721 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     3781 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     3841 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     3901 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     3961 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4021 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4081 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4141 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4201 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4261 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4321 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4381 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4441 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4501 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     4561 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     4621 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     4681 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     4741 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     4801 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     4861 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     4921 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     4981 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5041 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     5101 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5161 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5221 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5281 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5341 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     5401 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5461 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     5521 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     5581 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     5641 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     5701 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     5761 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     5821 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5881 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5941 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6061 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6601 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
     6661 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccg
//
