LOCUS       sequence-200509-       11840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Cas9 based efficient genome editing in S. aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11840)
  AUTHORS   Liu Q, Jiang Y, Shao L, Yang P, Sun B, Yang S, Chen D
  TITLE     CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus.
  JOURNAL   Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):764-770. doi: 
            10.1093/abbs/gmx074.
  PUBMED    28910979
REFERENCE   2  (bases 1 to 11840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Acta Biochim Biophys Sin (Shanghai)"; date: "2017-09-1- 1"; volume:
            "49"; issue: "9"; pages: "764-770"
COMMENT     Sequence Label: pLQ-Pxyl/tet-cas9-Pj23119-sgRNA
FEATURES             Location/Qualifiers
     source          1..11840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(147..735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(227..246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(906..1697,1698..1766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1529..1548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1767..1871)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1939..1957
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1995..2017)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2117..2136
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2330..2352
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2344..2361
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2345..2361
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4341..4967)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     4872..4891
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    4989..5007
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    5074..5092
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     promoter        5684..5718
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        5739..5814
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7393..7411
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(11585..11601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11585..11601)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11598..11620)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11609..11625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11633..11639,11640..11657,11658..11663))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11678..11699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11816..11833)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
       61 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
      121 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      181 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      241 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      301 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      361 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      421 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      481 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      541 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      601 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      661 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      721 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      781 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      841 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      901 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      961 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1021 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     1081 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1141 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1201 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1261 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1321 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1381 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1441 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1501 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1561 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1621 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1681 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1741 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1801 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     1861 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     1921 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     1981 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2041 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2101 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     2161 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     2221 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     2281 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     2341 cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc agaacggatt gttgatgatt
     2401 acgaaaatat taagagcaca gactattaca cagaaaatca agaattaaaa aaacgtagag
     2461 agagtttgaa agaagtagtg aatacatgga aagaggggta tcacgaaaaa agtaaagagg
     2521 ttaataaatt aaagcgagag aatgatagtt tgaatgagca gttgaatgta tcagagaaat
     2581 ttcaagatag tacagtgact ttatatcgtg ctgcgagggc gaatttccct gggtttgaga
     2641 aagggtttaa taggcttaaa gagaaattct ttaatgattc caaattcgag cgtgtgggac
     2701 agtttatgga tgttgtacag gataatgtcc agaaggtcga tagaaagcgt gagaaacagc
     2761 gtacagacga tttagagatg tagaggtact tttatgccga gaaaactttt tgcgtgtgac
     2821 agtccttaaa atatacttag agcgtaagcg aaagtagtag cgacagctat taactttcgg
     2881 ttgcaaagct ctaggatttt taatggacgc agcgcatcac acgcaaaaag gaaattggaa
     2941 taaatgcgaa atttgagatg ttaattaaag acctttttga ggtctttttt tcttagattt
     3001 ttggggttat ttaggggaga aaacataggg gggtactacg acctcccccc taggtgtcca
     3061 ttgtccattg tccaaacaaa taaataaata ttgggttttt aatgttaaaa ggttgttttt
     3121 tatgttaaag tgaaaaaaac agatgttggg aggtacagtg atggttgtag atagaaaaga
     3181 agagaaaaaa gttgctgtta ctttaagact tacaacagaa gaaaatgaga tattaaatag
     3241 aatcaaagaa aaatataata ttagcaaatc agatgcaacc ggtattctaa taaaaaaata
     3301 tgcaaaggag gaatacggtg cattttaaac aaaaaaagat agacagcact ggcatgctgc
     3361 ctatctatga ctaaattttg ttaaatgtat tagcaccgtt attatatcat gagcgaaaat
     3421 gtaataaaag aaactgaaaa caagaaaaat tcaagaggac gtaattggac atttgtttta
     3481 tatccagaat cagcaaaagc cgagtggtta gagtatttaa aagagttaca cattcaattt
     3541 gtagtgtctc cattacatga tagggatact gatacagaag ataggatgaa aaaagagcat
     3601 tatcatattc tagtgatgta tgagggtaat aaatcttatg aacagataaa aataattaca
     3661 gaagaattga atgcgactat tccgcagatt gcaggaagtg tgaaaggtct tgtgagatat
     3721 atgcttcaca tggacgatcc taataaattt aaatatcaaa aagaagatat gatagtttat
     3781 ggcggtgtag atgttgatga attattaaag aaaacaacaa cagatagata taaattaatt
     3841 aaagaaatga ttgagtttat tgatgaacaa ggaatcgtag aatttaagag tttaatggat
     3901 tatgcaatga agtttaaatt tgatgattgg ttcccgcttt tatgtgataa ctcggcgtat
     3961 gttattcaag aatatataaa atcaaatcgg tataaatctg accgatagat tttgaattta
     4021 agagtgtcac aagacactct tttttcgcac cagcgaaaac tggtttaagc cgactgcgca
     4081 aaagacataa tcgattcaca aaaaataggc acacgaaaaa caagttaagg gatgcagttt
     4141 atgcatccct taacttactt attaaataat ttatagctat tgaaaagaga taagaattgt
     4201 tcaaagctaa tattgtttaa atcgtcaatt cctgcatgtt ttaaggaatt gttaaattga
     4261 ttttttgtaa atattttctt gtattctttg ttatcttggt taccgtgaag ttaccatcac
     4321 ggaaaaaggt tatgctgctt ttaagaccca ctttcacatt taagttgttt ttctaatccg
     4381 catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat
     4441 tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct
     4501 ttagcgactt gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg
     4561 ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga
     4621 ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca
     4681 tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc
     4741 cagctttccc cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct
     4801 aaggcgtcga gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca
     4861 cctagcttct gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc
     4921 tctaatgcgc tgttaatcac tttactttta tctaatctag acatcattaa ttcctccttt
     4981 ttgttgacac tctatcattg atagagttat ttgtcaaact agttttttat ttggatcccc
     5041 tcgagttcat gaaaaactaa aaaaaatatt gacactctat cattgataga gtataattaa
     5101 aataagcttg atgggaatat gataatagaa ataccattac caacaccgaa ctgagtgatt
     5161 tgatcaccaa gccatattaa gaaagcagtt cctgctgtca aaactagtgc tattaataaa
     5221 taactcataa ttgactgatt gataatcagc gcacctttga gataattatt aaattggaat
     5281 gccataccta tagattggat aaatgctaaa gaaattgcta aataacgagt aacgttattt
     5341 aactttcttc tacctacttc accttgtttt gcccattctg agaatttagg gacaatatcc
     5401 atttgtaata attgcattac gattgatgca gtgatgtagg gtacaatacc cattgcaaaa
     5461 atagaaaatc gtttcaaggc tccgccacca aaagtattta ataactcagt ggcaccttga
     5521 gaaccttggg gattatcaaa agctgcagga tttactcctg gagctggtat ataagtccct
     5581 attttaaaaa ttactaacat tgctagtgtg aagaaaatct tgttacgaac ctcttttgtt
     5641 ctaaagaagt tcacaagggt ttgaatcatt agatcacaga tctttgacag ctagctcagt
     5701 cctaggtata atactagtat gtgttcgtat gttactgcgt tttagagcta gaaatagcaa
     5761 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     5821 tgcggccgcg tgatctaatg attcatatct acatcaaatg ctggggcata tgttttaccc
     5881 tcaggtacag gtaagtcacc cgcttcaaac gtcttcttaa tatgcgtaat tgcatctact
     5941 ctaaatccat caatgccttt atcaaaccac cagttcatca tttcaaatac agcatctcta
     6001 acttccggat taccccaatt caaatcaggt tgttttttac tgaataaatg gaaataatat
     6061 tgctcagtat tagcatcata ttcccgcgcg ccaattgagc tcccctttct atgtatgttt
     6121 tttactagtc atttaaaacg atacattaat aggtacgaaa aagcaacttt ttttgcgctt
     6181 aaaaccagtc ataccaataa cttaagggta actagcctcg ccggcaatag ttacccttat
     6241 tatcaagata agaaagaaaa ggatttttcg ctacgctcaa atcctttaaa aaaacacaaa
     6301 agaccacatt ttttaatgtg gtcttttatt cttcaactaa agcacccatt agttcaacaa
     6361 acgaaaattg gataaagtgg gatattttta aaatatatat ttatgttaca gtaatattga
     6421 cttttaaaaa aggattgatt ctaatgaaga aagcagacaa gtaagcctcc taaattcact
     6481 ttagataaaa atttaggagg catatcaaat gaaatttaat aaaattgatt tagacaattg
     6541 gaagagaaaa gagatattta atcattattt gaaccaacaa acgactttta gtataaccac
     6601 agaaattgat attagtgttt tataccgaaa cataaaacaa gaaggatata aattttaccc
     6661 tgcatttatt ttcttagtga caagggtgat aaactcaaat acagctttta gaactggtta
     6721 caatagcgac ggagagttag gttattggga taagttagag ccactttata caatttttga
     6781 tggtgtatct aaaacattct ctggtatttg gactcctgta aagaatgact tcaaagagtt
     6841 ttatgattta tacctttctg atgtagagaa atataatggt tcggggaaat tgtttcccaa
     6901 aacacctata cctgaaaatg ctttttctct ttctattatt ccatggactt catttactgg
     6961 gtttaactta aatatcaata ataatagtaa ttaccttcta cccattatta cagcaggaaa
     7021 attcattaat aaaggtaatt caatatattt accgctatct ttacaggtac atcattctgt
     7081 ttgtgatggt tatcatgcag gattgtttat gaactctatt caggaattgt cagataggcc
     7141 taatgactgg cttttataat atgagataat gccgactgta ctttttacag tcggttttct
     7201 aatgtcacta acctgccccg ttagttgaag aaggttttta tattacagct ccagatccat
     7261 atccttcttt ttctgaaccg acttctcctt tttcgcttct ttattccaat tgctttattg
     7321 acgttgagcc tcggaaccgt cgactttatt tggatcccct cgagttcatg aaaaactaaa
     7381 aaaaatattg aaactctatc attgatagag tataattaac aaggaggaat aaaaaatgga
     7441 taagaaatac tcaataggct tagatatcgg cacaaatagc gtcggatggg cggtgatcac
     7501 tgatgaatat aaggttccgt ctaaaaagtt caaggttctg ggaaatacag accgccacag
     7561 tatcaaaaaa aatcttatag gggctctttt atttgacagt ggagagacag cggaagcgac
     7621 tcgtctcaaa cggacagctc gtagaaggta tacacgtcgg aagaatcgta tttgttatct
     7681 acaggagatt ttttcaaatg agatggcgaa agtagatgat agtttctttc atcgacttga
     7741 agagtctttt ttggtggaag aagacaagaa gcatgaacgt catcctattt ttggaaatat
     7801 agtagatgaa gttgcttatc atgagaaata tctaactatc tatcatctgc gaaaaaaatt
     7861 ggtagattct actgataaag cggatttgcg cttaatctat ttggccttag cgcatatgat
     7921 taagtttcgt ggtcattttt tgattgaggg agatttaaat cctgataata gtgatgtgga
     7981 caaactattt atccagttgg tacaaaccta caatcaatta tttgaagaaa accctattaa
     8041 cgcaagtgga gtagatgcta aagcgattct ttctgcacga ttgagtaaat caagacgatt
     8101 agaaaatctc attgctcagc tccccggtga gaagaaaaat ggcttatttg ggaatctcat
     8161 tgctttgtca ttgggtttga cccctaattt taaatcaaat tttgatttgg cagaagatgc
     8221 taaattacag ctttcaaaag atacttacga tgatgattta gataatttat tggcgcaaat
     8281 tggagatcaa tatgctgatt tgtttttggc agctaagaat ttatcagatg ctattttact
     8341 ttcagatatc ctaagagtaa atactgaaat aactaaggct cccctatcag cttcaatgat
     8401 taaacgctac gatgaacatc atcaagactt gactctttta aaagctttag ttcgacaaca
     8461 acttccagaa aagtataaag aaatcttttt tgatcaatca aaaaacggat atgcaggtta
     8521 tattgatggg ggagctagcc aagaagaatt ttataaattt atcaaaccaa ttttagaaaa
     8581 aatggatggt actgaggaat tattggtgaa actaaatcgt gaagatttgc tgcgcaagca
     8641 acggaccttt gacaacggct ctattcccca tcaaattcac ttgggtgagc tgcatgctat
     8701 tttgagaaga caagaagact tttatccatt tttaaaagac aatcgtgaga agattgaaaa
     8761 aatcttgact tttcgaattc cttattatgt tggtccattg gcgcgtggca atagtcgttt
     8821 tgcatggatg actcggaagt ctgaagaaac aattacccca tggaattttg aagaagttgt
     8881 cgataaaggt gcttcagctc aatcatttat tgaacgcatg acaaactttg ataaaaatct
     8941 tccaaatgaa aaagtactac caaaacatag tttgctttat gagtatttta cggtttataa
     9001 cgaattgaca aaggtcaaat atgttactga aggaatgcga aaaccagcat ttctttcagg
     9061 tgaacagaag aaagccattg ttgatttact cttcaaaaca aatcgaaaag taaccgttaa
     9121 gcaattaaaa gaagattatt tcaaaaaaat agaatgtttt gatagtgttg aaatttcagg
     9181 agttgaagat agatttaatg cttcattagg tacctaccat gatttgctaa aaattattaa
     9241 agataaagat tttttggata atgaagaaaa tgaagatatc ttagaggata ttgttttaac
     9301 attgacctta tttgaagata gggagatgat tgaggaaaga cttaaaacat atgctcacct
     9361 ctttgatgat aaggtgatga aacagcttaa acgtcgccgt tatactggtt ggggacgttt
     9421 gtctcgaaaa ttgattaatg gtattaggga taagcaatct ggcaaaacaa tattagattt
     9481 tttgaaatca gatggttttg ccaatcgcaa ttttatgcag ctgatccatg atgatagttt
     9541 gacatttaaa gaagacattc aaaaagcaca agtgtctgga caaggcgata gtttacatga
     9601 acatattgca aatttagctg gtagccctgc tattaaaaaa ggtattttac agactgtaaa
     9661 agttgttgat gaattggtca aagtaatggg gcggcataag ccagaaaata tcgttattga
     9721 aatggcacgt gaaaatcaga caactcaaaa gggccagaaa aattcgcgag agcgtatgaa
     9781 acgaatcgaa gaaggtatca aagaattagg aagtcagatt cttaaagagc atcctgttga
     9841 aaatactcaa ttgcaaaatg aaaagctcta tctctattat ctccaaaatg gaagagacat
     9901 gtatgtggac caagaattag atattaatcg tttaagtgat tatgatgtcg atcacattgt
     9961 tccacaaagt ttccttaaag acgattcaat agacaataag gtcttaacgc gttctgataa
    10021 aaatcgtggt aaatcggata acgttccaag tgaagaagta gtcaaaaaga tgaaaaacta
    10081 ttggagacaa cttctaaacg ccaagttaat cactcaacgt aagtttgata atttaacgaa
    10141 agctgaacgt ggaggtttga gtgaacttga taaagctggt tttatcaaac gccaattggt
    10201 tgaaactcgc caaatcacta agcatgtggc acaaattttg gatagtcgca tgaatactaa
    10261 atacgatgaa aatgataaac ttattcgaga ggttaaagtg attaccttaa aatctaaatt
    10321 agtttctgac ttccgaaaag atttccaatt ctataaagta cgtgagatta acaattacca
    10381 tcatgcccat gatgcgtatc taaatgccgt cgttggaact gctttgatta agaaatatcc
    10441 aaaacttgaa tcggagtttg tctatggtga ttataaagtt tatgatgttc gtaaaatgat
    10501 tgctaagtct gagcaagaaa taggcaaagc aaccgcaaaa tatttctttt actctaatat
    10561 catgaacttc ttcaaaacag aaattacact tgcaaatgga gagattcgca aacgccctct
    10621 aatcgaaact aatggggaaa ctggagaaat tgtctgggat aaagggcgag attttgccac
    10681 agtgcgcaaa gtattgtcca tgccccaagt caatattgtc aagaaaacag aagtacagac
    10741 aggcggattc tccaaggagt caattttacc aaaaagaaat tcggacaagc ttattgctcg
    10801 taaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca
    10861 gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag
    10921 ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta
    10981 gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt
    11041 ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa
    11101 ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat
    11161 gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat
    11221 aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta
    11281 gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga caaaccaata
    11341 cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct
    11401 gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt
    11461 ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg
    11521 agtcagctag gaggtgactg aagtatattt tagatgaaga ttatttctta agaattctga
    11581 ctatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    11641 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    11701 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    11761 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    11821 tcactgactc gctgcgctcg
//
