LOCUS       sequence-200512-       12779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid has been discontinued. Please see the Depositor 
            Comments section for additional information..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12779)
  AUTHORS   Liu Q, Jiang Y, Shao L, Yang P, Sun B, Yang S, Chen D
  TITLE     CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus.
  JOURNAL   Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):764-770. doi: 
            10.1093/abbs/gmx074.
  PUBMED    28910979
REFERENCE   2  (bases 1 to 12779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Acta Biochim Biophys Sin (Shanghai)"; date: "2017-09-1- 1"; volume:
            "49"; issue: "9"; pages: "764-770"
COMMENT     Sequence Label: pLQ-kO-tgt
FEATURES             Location/Qualifiers
     source          1..12779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(43..631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(123..142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(802..1593,1594..1662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1425..1444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1663..1767)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1835..1853
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1891..1913)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2013..2032
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2226..2248
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2240..2257
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2241..2257
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(3992..4035)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3994..4075
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4094..4121
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      4201..4235
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     protein_bind    4534..4550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_RNA        4573..4648
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    6227..6245
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             6270..7988
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     primer_bind     complement(10419..10435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10419..10435)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10432..10454)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10443..10459
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10467..10473,10474..10491,10492..10497))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10512..10533
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12651..12668)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
       61 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
      121 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
      181 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
      241 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
      301 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
      361 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
      421 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
      481 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
      541 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
      601 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
      661 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
      721 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
      781 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
      841 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
      901 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
      961 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     1021 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     1081 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     1141 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     1201 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     1261 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     1321 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     1381 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     1441 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     1501 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     1561 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     1621 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     1681 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     1741 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     1801 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     1861 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     1921 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1981 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     2041 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     2101 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     2161 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     2221 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcagaa
     2281 cggattgttg atgattacga aaatattaag agcacagact attacacaga aaatcaagaa
     2341 ttaaaaaaac gtagagagag tttgaaagaa gtagtgaata catggaaaga ggggtatcac
     2401 gaaaaaagta aagaggttaa taaattaaag cgagagaatg atagtttgaa tgagcagttg
     2461 aatgtatcag agaaatttca agatagtaca gtgactttat atcgtgctgc gagggcgaat
     2521 ttccctgggt ttgagaaagg gtttaatagg cttaaagaga aattctttaa tgattccaaa
     2581 ttcgagcgtg tgggacagtt tatggatgtt gtacaggata atgtccagaa ggtcgataga
     2641 aagcgtgaga aacagcgtac agacgattta gagatgtaga ggtactttta tgccgagaaa
     2701 actttttgcg tgtgacagtc cttaaaatat acttagagcg taagcgaaag tagtagcgac
     2761 agctattaac tttcggttgc aaagctctag gatttttaat ggacgcagcg catcacacgc
     2821 aaaaaggaaa ttggaataaa tgcgaaattt gagatgttaa ttaaagacct ttttgaggtc
     2881 tttttttctt agatttttgg ggttatttag gggagaaaac ataggggggt actacgacct
     2941 cccccctagg tgtccattgt ccattgtcca aacaaataaa taaatattgg gtttttaatg
     3001 ttaaaaggtt gttttttatg ttaaagtgaa aaaaacagat gttgggaggt acagtgatgg
     3061 ttgtagatag aaaagaagag aaaaaagttg ctgttacttt aagacttaca acagaagaaa
     3121 atgagatatt aaatagaatc aaagaaaaat ataatattag caaatcagat gcaaccggta
     3181 ttctaataaa aaaatatgca aaggaggaat acggtgcatt ttaaacaaaa aaagatagac
     3241 agcactggca tgctgcctat ctatgactaa attttgttaa atgtattagc accgttatta
     3301 tatcatgagc gaaaatgtaa taaaagaaac tgaaaacaag aaaaattcaa gaggacgtaa
     3361 ttggacattt gttttatatc cagaatcagc aaaagccgag tggttagagt atttaaaaga
     3421 gttacacatt caatttgtag tgtctccatt acatgatagg gatactgata cagaagatag
     3481 gatgaaaaaa gagcattatc atattctagt gatgtatgag ggtaataaat cttatgaaca
     3541 gataaaaata attacagaag aattgaatgc gactattccg cagattgcag gaagtgtgaa
     3601 aggtcttgtg agatatatgc ttcacatgga cgatcctaat aaatttaaat atcaaaaaga
     3661 agatatgata gtttatggcg gtgtagatgt tgatgaatta ttaaagaaaa caacaacaga
     3721 tagatataaa ttaattaaag aaatgattga gtttattgat gaacaaggaa tcgtagaatt
     3781 taagagttta atggattatg caatgaagtt taaatttgat gattggttcc cgcttttatg
     3841 tgataactcg gcgtatgtta ttcaagaata tataaaatca aatcggtata aatctgaccg
     3901 atagattttg aatttaagag tgtcacaaga cactcttttt tcgcaccagc gaaaactggt
     3961 ttaagccgac tgcgcaaaag acatactcga gataaaacga aaggctcagt cgaaagactg
     4021 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     4081 gctctagcta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     4141 gttaactcta gagctgcctg ccgcgtttcg gtgatgaaga tcttcccgat gattaattaa
     4201 ttcagaacgc tcggttgccg ccgggcgttt tttatgcagc aatggcaaga acgttgctct
     4261 agaataattc tacacagccc agtccagact attcggcact gaaattatgg gtgaagtggt
     4321 caagacctca ctaggcacct taaaaatagc gcaccctgaa gaagatttat ttgaggtagc
     4381 ccttgcctac ctagcttcca agaaagatat cctaacagca caagagcgga aagatgtttt
     4441 gttctacatc cagaacaacc tctgctaaaa ttcctgaaaa attttgcaaa aagttgttga
     4501 ctttatctac aaggtgtggc ataatgtgtg gaattgtgag cggataacaa ttatgtgttc
     4561 gtatgttact gcgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     4621 acttgaaaaa gtggcaccga gtcggtgctt tttttgcggc cgcgtgatct aatgattcat
     4681 atctacatca aatgctgggg catatgtttt accctcaggt acaggtaagt cacccgcttc
     4741 aaacgtcttc ttaatatgcg taattgcatc tactctaaat ccatcaatgc ctttatcaaa
     4801 ccaccagttc atcatttcaa atacagcatc tctaacttcc ggattacccc aattcaaatc
     4861 aggttgtttt ttactgaata aatggaaata atattgctca gtattagcat catattcccg
     4921 cgcgccaatt gagctcccct ttctatgtat gttttttact agtcatttaa aacgatacat
     4981 taataggtac gaaaaagcaa ctttttttgc gcttaaaacc agtcatacca ataacttaag
     5041 ggtaactagc ctcgccggca atagttaccc ttattatcaa gataagaaag aaaaggattt
     5101 ttcgctacgc tcaaatcctt taaaaaaaca caaaagacca cattttttaa tgtggtcttt
     5161 tattcttcaa ctaaagcacc cattagttca acaaacgaaa attggataaa gtgggatatt
     5221 tttaaaatat atatttatgt tacagtaata ttgactttta aaaaaggatt gattctaatg
     5281 aagaaagcag acaagtaagc ctcctaaatt cactttagat aaaaatttag gaggcatatc
     5341 aaatgaaatt taataaaatt gatttagaca attggaagag aaaagagata tttaatcatt
     5401 atttgaacca acaaacgact tttagtataa ccacagaaat tgatattagt gttttatacc
     5461 gaaacataaa acaagaagga tataaatttt accctgcatt tattttctta gtgacaaggg
     5521 tgataaactc aaatacagct tttagaactg gttacaatag cgacggagag ttaggttatt
     5581 gggataagtt agagccactt tatacaattt ttgatggtgt atctaaaaca ttctctggta
     5641 tttggactcc tgtaaagaat gacttcaaag agttttatga tttatacctt tctgatgtag
     5701 agaaatataa tggttcgggg aaattgtttc ccaaaacacc tatacctgaa aatgcttttt
     5761 ctctttctat tattccatgg acttcattta ctgggtttaa cttaaatatc aataataata
     5821 gtaattacct tctacccatt attacagcag gaaaattcat taataaaggt aattcaatat
     5881 atttaccgct atctttacag gtacatcatt ctgtttgtga tggttatcat gcaggattgt
     5941 ttatgaactc tattcaggaa ttgtcagata ggcctaatga ctggctttta taatatgaga
     6001 taatgccgac tgtacttttt acagtcggtt ttctaatgtc actaacctgc cccgttagtt
     6061 gaagaaggtt tttatattac agctccagat ccatatcctt ctttttctga accgacttct
     6121 cctttttcgc ttctttattc caattgcttt attgacgttg agcctcggaa ccgtcgactt
     6181 tatttggatc ccctcgagtt catgaaaaac taaaaaaaat attgacactc tatcattgat
     6241 agagtataat taacaaggag gaataaaaaa tggataagaa atactcaata ggcttagata
     6301 tcggcacaaa tagcgtcgga tgggcggtga tcactgatga atataaggtt ccgtctaaaa
     6361 agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt ataggggctc
     6421 ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca gctcgtagaa
     6481 ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca aatgagatgg
     6541 cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg gaagaagaca
     6601 agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct tatcatgaga
     6661 aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat aaagcggatt
     6721 tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat tttttgattg
     6781 agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag ttggtacaaa
     6841 cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat gctaaagcga
     6901 ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct cagctccccg
     6961 gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt ttgaccccta
     7021 attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca aaagatactt
     7081 acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct gatttgtttt
     7141 tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga gtaaatactg
     7201 aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa catcatcaag
     7261 acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat aaagaaatct
     7321 tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct agccaagaag
     7381 aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag gaattattgg
     7441 tgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac ggctctattc
     7501 cccatcaaat tcacttgggt gagctgcatg ctattttgag aagacaagaa gacttttatc
     7561 catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga attccttatt
     7621 atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg aagtctgaag
     7681 aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca gctcaatcat
     7741 ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta ctaccaaaac
     7801 atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc aaatatgtta
     7861 ctgaaggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc attgttgatt
     7921 tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat tatttcaaaa
     7981 aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt aatgcttcat
     8041 taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg gataatgaag
     8101 aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa gatagggaga
     8161 tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg atgaaacagc
     8221 ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt aatggtatta
     8281 gggataagca atctggcaaa acaatattag attttttgaa atcagatggt tttgccaatc
     8341 gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac attcaaaaag
     8401 cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta gctggtagcc
     8461 ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg gtcaaagtaa
     8521 tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat cagacaactc
     8581 aaaagggcca gaaaaattcg cgagagcgta tgaaacgaat cgaagaaggt atcaaagaat
     8641 taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc
     8701 tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa ttagatatta
     8761 atcgtttaag tgattatgat gtcgatcaca ttgttccaca aagtttcctt aaagacgatt
     8821 caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg gataacgttc
     8881 caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta aacgccaagt
     8941 taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt ttgagtgaac
     9001 ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc actaagcatg
     9061 tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat aaacttattc
     9121 gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga aaagatttcc
     9181 aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg tatctaaatg
     9241 ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag tttgtctatg
     9301 gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcaa gaaataggca
     9361 aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa acagaaatta
     9421 cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg gaaactggag
     9481 aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg tccatgcccc
     9541 aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag gagtcaattt
     9601 taccaaaaag aaattcggac aagcttattg ctcgtaaaaa gactgggatc caaaaaaata
     9661 tggtggtttt gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa
     9721 agggaaatcg aagaagttaa aatccgttaa agagttacta gggatcacaa ttatggaaag
     9781 aagttccttt gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa
     9841 aaaagactta atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa
     9901 acggatgctg gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa
     9961 atatgtgaat tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga
    10021 taacgaacaa aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga
    10081 gcaaatcagt gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct
    10141 tagtgcatat aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca
    10201 tttatttacg ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat
    10261 tgatcgtaaa cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc
    10321 catcactggt ctttatgaaa cacgcattga tttgagtcag ctaggaggtg actgaagtat
    10381 attttagatg aagattattt cttaagaatt ctgactatgt catagctgtt tcctgtgtga
    10441 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10501 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10561 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10621 ggtttgcgta ttgggcgctc ttccaaacgc ctttaaaaga tcgtgatcat agtcgtttat
    10681 tagtcatgga tagagaaact ggtgaaatga aacatttaca tttcaaagat atcattgagt
    10741 attttagacc tggtgataca ttagtgctta acgatacgcg agtaatgcca gctagacttt
    10801 ttggtttaaa agaagaaact ggtgcaaaag ttgaaatgtt aatgttaact caaattgaag
    10861 gtaatgattg ggaagtctta ctgaaaccag ctaagcgtat taaagttggt aataaattga
    10921 attttggtaa tggcaaaatt atagctgaat gcataaaaga aatggatcaa ggtggacgca
    10981 tcatgcgttt acattatgaa ggtattttac aagaaagatt agatgaatta ggggaaatgc
    11041 cactgccacc atacatcaaa gaacgtttag atgatccaga tcgttatcaa acagtttacg
    11101 ctaaagaaag tggttcagcg gcagcaccaa cagcaggatt acattttact gatgagttat
    11161 taattgaaat taaaaataaa ggtgttaata tcgcatttgt tacattacat gttgggttag
    11221 gtacgtttag accggtgagc gtagacgatg tgaatgacca cgaaatgcat agtgaatatt
    11281 atcaaatgac acaagaaaca gctgatttat taaatgatac taagtccaaa ggacatcgca
    11341 ttatatcagt tggtacaact tcaacacgta cacttgaaac aattcgacgc gatcatgata
    11401 aatttgttga aacgagtggc tggactaata tatttattta tccaggattt gattttaaag
    11461 caattgatgg ccagattact aattttcatt taccaaaatc aacattagtt atgctagtat
    11521 cagcgtttag tagtcgtgaa aatgttctga atgcttataa aacggcagta aatttagaat
    11581 atagattctt tagttttggc gatgcaatgt taattatata aaaagaatgt gaggattttg
    11641 aatgcaagag gagcgtataa aatgcaattt tcattactaa tatatatagt cgtaattttt
    11701 gcggttatgt atttcttgat gatcagacca caacaaaaac gtgcgaaaca gcatcgtgag
    11761 ttgattaata acattcaatc tggtcaaaga attacaacta ttggtggtat taaaggtact
    11821 gttaaagcag tagatgaaac aactgttgtt attacagtta atggtcatgg tactgaatta
    11881 actttcgaaa aacctgctat taaacaagtt gacccttcat aatgagaagt ggtattaaaa
    11941 aggatgaaac gaatagcgtt tcatcctttt taagttataa gtagtatgta gaatagctta
    12001 tttttattcc aaaaaaagaa attgtgatac ataataattg ttaaatttaa attaaatata
    12061 tctatgacta tttttactcc ctgctctagt atgttaatat gtataaggct aataaatttt
    12121 gtaagaaaga tttaacttgc gttataaaat agtattcaag tattctaaat acatacgtaa
    12181 ataatgaggt gttcatgtga agaaaagtag tagaataatt gcgttcttgc ttttagtcgt
    12241 attgttgttt gccggaatgg ctgcaactta taaaagtgtt ataaagaatg tcaatttagg
    12301 acttgatttg caaggtggat ttgaggtgct ttatcaagtc gatcctttaa ataaaggcga
    12361 caaaatagat aaaaaagcct tacaatcaac agcgcaaaca ttagaaaatc gtgttaacgt
    12421 tttaggtgtt tcggaaccta aaattcaagt tgaagaacct aatagaatta gagtacaact
    12481 tgctggtgta acagaccaga atgaagcacg taaaatttta tcttctcaag ctaatttaac
    12541 aattcgcgat gcagaagata aagtaaaatt aagcggttct gatattaaac agggatctgc
    12601 caaacaagaa tttaaacagg aaacaaacca gcctacagtt actcccgggc ctcgctcact
    12661 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    12721 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggcca
//
