LOCUS       sequence-200518-        3929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding mRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-mRFP1
FEATURES             Location/Qualifiers
     source          1..3929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             164..835
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TG"
     terminator      897..943
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1116..2066)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2114..2336
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2842..2876)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2917..3711)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3028..3047)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3638..3657
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3890..3908
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacaccacgt cgtccctatc tgctgcccta ggtctatgag tggttgctgg ataactttac
       61 gggcatgcat aaggctcgta tgatatattc agggagacca caacggtttc cctctacaaa
      121 taattttgtt taacttttct agatttaaga aggagatata catatggcca gcagcgagga
      181 cgtgattaaa gagttcatgc gcttcaaagt tcgcatggag ggttctgtta acggtcacga
      241 gttcgagatc gaaggcgaag gcgagggccg tccgtatgaa ggcacccaga ccgccaaact
      301 gaaagtgact aaaggcggcc cgctgccttt tgcgtgggac atcctgagcc cgcaatttca
      361 gtacggttct aaagcgtatg ttaaacaccc agcggatatc ccggactatc tgaagctgtc
      421 ttttccggaa ggtttcaagt gggaacgcgt aatgaatttt gaagatggtg gtgtcgtgac
      481 cgtcactcag gactcctccc tgcaggatgg cgagttcatc tataaagtta aactgcgtgg
      541 tactaatttt ccatctgatg gcccggtgat gcagaaaaag acgatgggtt gggaggcgtc
      601 taccgaacgc atgtatccgg aagatggtgc gctgaaaggc gaaattaaaa tgcgcctgaa
      661 actgaaagat ggcggccatt atgacgctga agtgaaaacc acgtacatgg ccaagaaacc
      721 tgtgcagctg cctggcgcgt acaagaccga tattaaactg gacatcacct ctcataatga
      781 agattatacg atcgtagagc aatatgagcg cgcggagggt cgtcattcta ccggtggcat
      841 ggatgaacta tacaaataaa tgtccagacc tgcaggcatg caagctctag aggcatcaaa
      901 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttga aatgcaccaa
      961 aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg
     1021 acagttttcc ctttgatatc taacggtgaa cagttgttct acttttgttt gttagtcttg
     1081 atgcttcact gatagataca agagccataa gaacctcaga tccttccgta tttagccagt
     1141 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
     1201 tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
     1261 gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg
     1321 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
     1381 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
     1441 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
     1501 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
     1561 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
     1621 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
     1681 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
     1741 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
     1801 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
     1861 acaccataag cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac
     1921 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
     1981 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
     2041 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
     2101 taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg agttgtgggt
     2161 atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta
     2221 aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga
     2281 ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta
     2341 ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa
     2401 aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg
     2461 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca
     2521 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt
     2581 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg
     2641 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
     2701 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
     2761 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtcaacca
     2821 gacccgccag gattctcatg cataaaaaac gcccggcggc aaccgagcgt tctgaattaa
     2881 ttaatcatcg cgactgcaga gatcccagag tccttattag aagaactcgt caagaaggcg
     2941 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     3001 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     3061 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     3121 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     3181 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     3241 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     3301 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3361 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     3421 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3481 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     3541 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     3601 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     3661 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     3721 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     3781 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     3841 caattccgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3901 cacgaggccc tttcgtcttc accacagct
//
