LOCUS       sequence-200519-        3941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding mCherry-L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-mCherry-L
FEATURES             Location/Qualifiers
     source          1..3941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      936..982
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1155..2105)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2153..2375
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2881..2915)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2956..3750)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3067..3086)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3677..3696
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     join(3929..3941,1..6)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgaggccctt tcgtcttcac cacagctaac accacgtcgt ccctatctgc tgccctaggt
       61 ctatgagtgg ttgctggata actttacggg catgcataag gctcgtatga tatattcagg
      121 gagaccacaa cggtttccct ctacaaataa ttttgtttaa cttttctaga tttaagaagg
      181 agatatacat atgagtaaag gagaagaaga taacttagct atcattaaag agttcatgcg
      241 cttcaaagtt cacatggagg gttctgttaa cggtcacgag ttcgagatcg aaggcgaagg
      301 cgagggccgt ccgtatgaag gcacccagac cgccaaactg aaagtgacta aaggcggccc
      361 gctgcctttt gcgtgggaca tcctgagccc gcaatttatg tacggttcta aagcgtatgt
      421 taaacaccca gcggatatcc cggactatct gaagctgtct tttccggaag gtttcaagtg
      481 ggaacgcgta atgaattttg aagatggtgg tgtcgtgacc gtcactcagg actcctccct
      541 gcaggatggc gagttcatct ataaagttaa actgcgtggt actaattttc catctgatgg
      601 cccggtgatg cagaaaaaga cgatgggttg ggaggcgtct agcgaacgca tgtatccgga
      661 agatggtgcg ctgaaaggcg aaattaaaca gcgcctgaaa ctgaaagatg gcggccatta
      721 tgacgctgaa gtgaaaacca cgtacaaagc caagaaacct gtgcagctgc ctggcgcgta
      781 caatgtgaat attaaactgg acatcacctc tcataatgaa gattatacga tcgtagagca
      841 atatgagcgc gcggagggtc gtcattctac cggtggcatg gatgaactat acaaataaat
      901 gtccagacct gcaggcatgc aagctctaga ggcatcaaat aaaacgaaag gctcagtcga
      961 aagactgggc ctttcgtttt atctgttgaa atgcaccaaa aactcgtaaa agctctgatg
     1021 tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc tttgatatct
     1081 aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa
     1141 gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt
     1201 tgtttttgcg tgagccatga gaacgaacca ttgagatcat gcttactttg catgtcactc
     1261 aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt
     1321 tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca
     1381 ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca
     1441 acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg
     1501 ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac
     1561 tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata
     1621 tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata
     1681 gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac
     1741 tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac
     1801 ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc
     1861 acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc attttcccta
     1921 ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt
     1981 gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca
     2041 tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca
     2101 gacatacatc tcaattggtc taggtgattt taatcactat accaattgag atgggctagt
     2161 caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc
     2221 tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt
     2281 gttttttttg tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag
     2341 ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat
     2401 tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa
     2461 ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt tgtctccgac
     2521 catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc tcacggctct
     2581 ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg caaggaaact
     2641 acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg cgggtctgct
     2701 atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt ttccagtctg
     2761 accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc agtaaggcag
     2821 cggtatcatc aacaggctta cccgtcttac tgtcaaccag acccgccagg attctcatgc
     2881 ataaaaaacg cccggcggca accgagcgtt ctgaattaat taatcatcgc gactgcagag
     2941 atcccagagt ccttattaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     3001 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     3061 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     3121 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     3181 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
     3241 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     3301 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     3361 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     3421 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     3481 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     3541 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     3601 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     3661 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     3721 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     3781 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     3841 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg tctaagaaac
     3901 cattattatc atgacattaa cctataaaaa taggcgtatc a
//
