LOCUS       sequence-200520-        3941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding mCherry2-L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-mCherry2-L
FEATURES             Location/Qualifiers
     source          1..3941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..48
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      978..1024
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1197..2147)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2195..2417
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2923..2957)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2998..3792)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3109..3128)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3719..3738
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
       61 accacagcta acaccacgtc gtccctatct gctgccctag gtctatgagt ggttgctgga
      121 taactttacg ggcatgcata aggctcgtat gatatattca gggagaccac aacggtttcc
      181 ctctacaaat aattttgttt aacttttcta gatttaagaa ggagatatac atatgagtaa
      241 aggagaagaa aacaacttag ctatcattaa agagttcatg cgcttcaaag ttcacatgga
      301 gggttctgtt aacggtcacg agttcgagat cgaaggcgaa ggcgagggcc gtccgtatga
      361 aggcacccag accgccaaac tgaaagtgac taaaggcggc ccgctgcctt ttgcgtggga
      421 catcctgagc ccgcaattta tgtacggttc taaagcgtat gttaaacacc cagcggatat
      481 cccggactat ctgaagctgt cttttccgga aggtttcaac tgggaacgcg taatgaattt
      541 tgaagatggt ggtgtcgtga ccgtcactca ggactcctcc ctgcaggatg gcgagttcat
      601 ctataaagtt aaactgcgtg gtactaattt tccatctgat ggcccggtga tgcagtgtag
      661 gacgatgggt tgggaggcgt ctaccgaacg catgtatccg gaagatggtg cgctgaaagg
      721 cgaaattaaa cagcgcctga aactgaaaga tggcggccat tatgacgctg aagtgaaaac
      781 cacgtacaaa gccaagaaac ctgtgcagct gcctggcgcg tacaatgtgg atattaaact
      841 ggacatctta tctcataatg aagattatac gatcgtagag caatatgagc gcgcggaggg
      901 tcgtcattct accggtggca tggatgaact atacaaataa atgtccagac ctgcaggcat
      961 gcaagctcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1021 ttatctgttg aaatgcacca aaaactcgta aaagctctga tgtatctatc ttttttacac
     1081 cgttttcatc tgtgcatatg gacagttttc cctttgatat ctaacggtga acagttgttc
     1141 tacttttgtt tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag
     1201 atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat
     1261 gagaacgaac cattgagatc atgcttactt tgcatgtcac tcaaaaattt tgcctcaaaa
     1321 ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttacgt
     1381 aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg
     1441 ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta
     1501 tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct
     1561 ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca
     1621 agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc
     1681 ttttgtgtta gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa
     1741 gacttaacat gttccagatt atattttatg aattttttta actggaaaag ataaggcaat
     1801 atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac
     1861 tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc
     1921 tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga
     1981 gcgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg
     2041 gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag
     2101 cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg
     2161 tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc
     2221 cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta
     2281 aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt
     2341 caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc
     2401 agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa
     2461 acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc
     2521 gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc acctgagtcg
     2581 ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga atgggggtaa
     2641 atggcactac aggcgccttt tatggattca tgcaaggaaa ctacccataa tacaagaaaa
     2701 gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg ctatgtggtg ctatctgact
     2761 ttttgctgtt cagcagttcc tgccctctga ttttccagtc tgaccacttc ggattatccc
     2821 gtgacaggtc attcagactg gctaatgcac ccagtaaggc agcggtatca tcaacaggct
     2881 tacccgtctt actgtcaacc agacccgcca ggattctcat gcataaaaaa cgcccggcgg
     2941 caaccgagcg ttctgaatta attaatcatc gcgactgcag agatcccaga gtccttatta
     3001 gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc
     3061 gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt
     3121 agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc
     3181 agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac
     3241 gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag
     3301 cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg
     3361 tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt
     3421 atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga
     3481 tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt
     3541 gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc
     3601 tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg
     3661 gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc
     3721 ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc
     3781 ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg atcccctgcg
     3841 ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct tcccaacctt
     3901 accagagggc gccccagctg gcaattccga cgtctaagaa a
//
