LOCUS       sequence-200524-        3929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-mScarlet-I
FEATURES             Location/Qualifiers
     source          1..3929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     85..103
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1021..1067
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1240..2190)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2238..2460
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2966..3000)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3041..3835)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3152..3171)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3762..3781
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccgacgtcta agaaaccatt
       61 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaccac
      121 agctaacacc acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataact
      181 ttacgggcat gcataaggct cgtatgatat attcagggag accacaacgg tttccctcta
      241 caaataattt tgtttaactt ttctagattt aagaaggaga tatacatatg agtaaaggag
      301 aagctgtgat taaagagttc atgcgcttca aagttcacat ggagggttct atgaacggtc
      361 acgagttcga gatcgaaggc gaaggcgagg gccgtccgta tgaaggcacc cagaccgcca
      421 aactgaaagt gactaaaggc ggcccgctgc ctttttcctg ggacatcctg agcccgcaat
      481 ttatgtacgg ttctagggcg ttcatcaaac acccagcgga tatcccggac tattataagc
      541 agtcttttcc ggaaggtttc aagtgggaac gcgtaatgaa ttttgaagat ggtggtgccg
      601 tgaccgtcac tcaggacacc tccctggagg atggcaccct gatctataaa gttaaactgc
      661 gtggtactaa ttttccacct gatggcccgg tgatgcagaa aaagacgatg ggttgggagg
      721 cgtctaccga acgcttgtat ccggaagatg gtgtgctgaa aggcgacatt aaaatggccc
      781 tgcgcctgaa agatggcggc cgctatctgg ctgacttcaa aaccacgtac aaagccaaga
      841 aacctgtgca gatgcctggc gcgtacaatg tggaccgcaa actggacatc acctctcata
      901 atgaagatta tacggtggta gagcaatatg agcgctccga gggtcgtcat tctaccggtg
      961 gcatggatga actatacaaa taaatgtcca gacctgcagg catgcaagct ctagaggcat
     1021 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgaaatgca
     1081 ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc atctgtgcat
     1141 atggacagtt ttccctttga tatctaacgg tgaacagttg ttctactttt gtttgttagt
     1201 cttgatgctt cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc
     1261 cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag
     1321 atcatgctta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt
     1381 tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta cgtaggtagg aatctgatgt
     1441 aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac
     1501 gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg
     1561 cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa
     1621 aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt
     1681 aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt
     1741 taataaccac tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag
     1801 attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac
     1861 taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc
     1921 tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc
     1981 taatacacca taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt
     2041 gaacgatacc gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac
     2101 acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc
     2161 atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc
     2221 actataccaa ttgagatggg ctagtcaatg ataattacta gtccttttcc tttgagttgt
     2281 gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg ctagaccctc
     2341 tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg ttataattta
     2401 tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg tgtataactc
     2461 actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt ttgctcctct
     2521 acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc
     2581 gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca
     2641 ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac tacaggcgcc
     2701 ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc acgggcttct
     2761 cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct gttcagcagt
     2821 tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag gtcattcaga
     2881 ctggctaatg cacccagtaa ggcagcggta tcatcaacag gcttacccgt cttactgtca
     2941 accagacccg ccaggattct catgcataaa aaacgcccgg cggcaaccga gcgttctgaa
     3001 ttaattaatc atcgcgactg cagagatccc agagtcctta ttagaagaac tcgtcaagaa
     3061 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     3121 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     3181 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     3241 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     3301 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     3361 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     3421 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     3481 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     3541 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     3601 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     3661 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     3721 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     3781 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     3841 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
     3901 gcaagaaagc catccagttt actttgcag
//
