LOCUS       sequence-200531-        3941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding mRFP1*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-mRFP1*
FEATURES             Location/Qualifiers
     source          1..3941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..96
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1026..1072
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1245..2195)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2243..2465
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2971..3005)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3046..3840)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3157..3176)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3767..3786
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaccttacc agagggcgcc ccagctggca attccgacgt ctaagaaacc attattatca
       61 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac cacagctaac
      121 accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata actttacggg
      181 catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct ctacaaataa
      241 ttttgtttaa cttttctaga tttaagaagg agatatacat atgagtaaag gagaagaaaa
      301 caacttagct gtgattaaag agttcatgcg cttcaaagtt cgcatggagg gttctgttaa
      361 cggtcacgag ttcgagatcg aaggcgaagg cgagggccgt ccgtatgaag gcacccagac
      421 cgccaaactg aaagtgacta aaggcggccc gctgcctttt gcgtgggaca tcctgagccc
      481 gcaatttcag tacggttcta aagcgtatgt taaacaccca gcggatatcc cggactatct
      541 gaagctgtct tttccggaag gtttcaagtg ggaacgcgta atgaattttg aagatggtgg
      601 tgtcgtgacc gtcactcagg actcctccct gcaggatggc gagttcatct ataaagttaa
      661 actgcgtggt actaattttc catctgatgg cccggtgatg cagaaaaaga cgatgggttg
      721 ggaggcgtct accgaacgca tgtatccgga agatggtgcg ctgaaaggcg aaattaaaat
      781 gcgcctgaaa ctgaaagatg gcggccatta tgacgctgaa gtgaaaacca cgtacatggc
      841 caagaaacct gtgcagctgc ctggcgcgta caagaccgat attaaactgg acatcacctc
      901 tcataatgaa gattatacga tcgtagagca atatgagcgc gcggagggtc gtcattctac
      961 cggtggcatg gatgaactat acaaataaat gtccagacct gcaggcatgc aagctctaga
     1021 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgaa
     1081 atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg
     1141 tgcatatgga cagttttccc tttgatatct aacggtgaac agttgttcta cttttgtttg
     1201 ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat
     1261 ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca
     1321 ttgagatcat gcttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga
     1381 atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct
     1441 gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg
     1501 gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg
     1561 cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt
     1621 ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg
     1681 aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt
     1741 tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt
     1801 tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta
     1861 aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca
     1921 aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct
     1981 ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta
     2041 taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt
     2101 gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct
     2161 agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt
     2221 taatcactat accaattgag atgggctagt caatgataat tactagtcct tttcctttga
     2281 gttgtgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga
     2341 ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca agtggttata
     2401 atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat
     2461 aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct
     2521 cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc
     2581 aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg
     2641 tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag
     2701 gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg
     2761 cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca
     2821 gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat
     2881 tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta cccgtcttac
     2941 tgtcaaccag acccgccagg attctcatgc ataaaaaacg cccggcggca accgagcgtt
     3001 ctgaattaat taatcatcgc gactgcagag atcccagagt ccttattaga agaactcgtc
     3061 aagaaggcga tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag
     3121 gaagcggtca gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat
     3181 gtcctgatag cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc
     3241 attttccacc atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc
     3301 gtcgggcatg cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc
     3361 ttcgtccaga tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat
     3421 gcgatgtttc gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg
     3481 cattgcatca gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc
     3541 ctgccccggc acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag
     3601 cacagctgcg caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg
     3661 cagttcattc agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc
     3721 tgacagccgg aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc
     3781 gaatagcctc tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat
     3841 gcgaaacgat cctcatcctg tctcttgatc agatcttgat cccctgcgcc atcagatcct
     3901 tggcggcaag aaagccatcc agtttacttt gcagggcttc c
//
