LOCUS       sequence-200536-        3944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding 2ndVal mCherry2-L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-2ndVal mCherry2-L
FEATURES             Location/Qualifiers
     source          1..3944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..73
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1006..1052
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1225..2175)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2223..2445
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2951..2985)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3026..3820)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3137..3156)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3747..3766
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gctggcaatt ccgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
       61 cgtatcacga ggccctttcg tcttcaccac agctaacacc acgtcgtccc tatctgctgc
      121 cctaggtcta tgagtggttg ctggataact ttacgggcat gcataaggct cgtatgatat
      181 attcagggag accacaacgg tttccctcta caaataattt tgtttaactt ttctagattt
      241 aagaaggaga tatacatatg gtgagtaaag gagaagaaaa caacttagct atcattaaag
      301 agttcatgcg cttcaaagtt cacatggagg gttctgttaa cggtcacgag ttcgagatcg
      361 aaggcgaagg cgagggccgt ccgtatgaag gcacccagac cgccaaactg aaagtgacta
      421 aaggcggccc gctgcctttt gcgtgggaca tcctgagccc gcaatttatg tacggttcta
      481 aagcgtatgt taaacaccca gcggatatcc cggactatct gaagctgtct tttccggaag
      541 gtttcaactg ggaacgcgta atgaattttg aagatggtgg tgtcgtgacc gtcactcagg
      601 actcctccct gcaggatggc gagttcatct ataaagttaa actgcgtggt actaattttc
      661 catctgatgg cccggtgatg cagtgtagga cgatgggttg ggaggcgtct accgaacgca
      721 tgtatccgga agatggtgcg ctgaaaggcg aaattaaaca gcgcctgaaa ctgaaagatg
      781 gcggccatta tgacgctgaa gtgaaaacca cgtacaaagc caagaaacct gtgcagctgc
      841 ctggcgcgta caatgtggat attaaactgg acatcttatc tcataatgaa gattatacga
      901 tcgtagagca atatgagcgc gcggagggtc gtcattctac cggtggcatg gatgaactat
      961 acaaataaat gtccagacct gcaggcatgc aagctctaga ggcatcaaat aaaacgaaag
     1021 gctcagtcga aagactgggc ctttcgtttt atctgttgaa atgcaccaaa aactcgtaaa
     1081 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc
     1141 tttgatatct aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
     1201 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
     1261 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat gcttactttg
     1321 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
     1381 gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat
     1441 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
     1501 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
     1561 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
     1621 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
     1681 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
     1741 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
     1801 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
     1861 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
     1921 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
     1981 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
     2041 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
     2101 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
     2161 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
     2221 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
     2281 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
     2341 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
     2401 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
     2461 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
     2521 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt
     2581 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
     2641 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
     2701 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg
     2761 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt
     2821 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc
     2881 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtcaaccag acccgccagg
     2941 attctcatgc ataaaaaacg cccggcggca accgagcgtt ctgaattaat taatcatcgc
     3001 gactgcagag atcccagagt ccttattaga agaactcgtc aagaaggcga tagaaggcga
     3061 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     3121 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     3181 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     3241 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     3301 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     3361 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     3421 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     3481 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     3541 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     3601 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg
     3661 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     3721 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     3781 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg
     3841 tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc
     3901 agtttacttt gcagggcttc ccaaccttac cagagggcgc ccca
//
