LOCUS       sequence-200537-        3932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding 2ndVal mScarlet-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Balleza E, Kim JM, Cluzel P
  TITLE     Systematic characterization of maturation time of fluorescent 
            proteins in living cells.
  JOURNAL   Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 
            2017 Nov 20.
  PUBMED    29320486
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.4509"; journalName: "Nat Methods"; date: "2018-01"; 
            volume: "15"; issue: "1"; pages: "47-51"
COMMENT     Sequence Label: pEB2-2ndVal mScarlet-I
FEATURES             Location/Qualifiers
     source          1..3932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     78..96
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      1017..1063
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1236..2186)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      2234..2456
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2962..2996)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(3037..3831)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3148..3167)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3758..3777
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 caaccttacc agagggcgcc ccagctggca attccgacgt ctaagaaacc attattatca
       61 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac cacagctaac
      121 accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata actttacggg
      181 catgcataag gctcgtatga tatattcagg gagaccacaa cggtttccct ctacaaataa
      241 ttttgtttaa cttttctaga tttaagaagg agatatacat atggtgagta aaggagaagc
      301 tgtgattaaa gagttcatgc gcttcaaagt tcacatggag ggttctatga acggtcacga
      361 gttcgagatc gaaggcgaag gcgagggccg tccgtatgaa ggcacccaga ccgccaaact
      421 gaaagtgact aaaggcggcc cgctgccttt ttcctgggac atcctgagcc cgcaatttat
      481 gtacggttct agggcgttca tcaaacaccc agcggatatc ccggactatt ataagcagtc
      541 ttttccggaa ggtttcaagt gggaacgcgt aatgaatttt gaagatggtg gtgccgtgac
      601 cgtcactcag gacacctccc tggaggatgg caccctgatc tataaagtta aactgcgtgg
      661 tactaatttt ccacctgatg gcccggtgat gcagaaaaag acgatgggtt gggaggcgtc
      721 taccgaacgc ttgtatccgg aagatggtgt gctgaaaggc gacattaaaa tggccctgcg
      781 cctgaaagat ggcggccgct atctggctga cttcaaaacc acgtacaaag ccaagaaacc
      841 tgtgcagatg cctggcgcgt acaatgtgga ccgcaaactg gacatcacct ctcataatga
      901 agattatacg gtggtagagc aatatgagcg ctccgagggt cgtcattcta ccggtggcat
      961 ggatgaacta tacaaataaa tgtccagacc tgcaggcatg caagctctag aggcatcaaa
     1021 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttga aatgcaccaa
     1081 aaactcgtaa aagctctgat gtatctatct tttttacacc gttttcatct gtgcatatgg
     1141 acagttttcc ctttgatatc taacggtgaa cagttgttct acttttgttt gttagtcttg
     1201 atgcttcact gatagataca agagccataa gaacctcaga tccttccgta tttagccagt
     1261 atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc attgagatca
     1321 tgcttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg aatttttgca
     1381 gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg
     1441 gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc ggttacgaga
     1501 tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg gcctcgctta
     1561 tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg tttcaaaacc
     1621 cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat gaacttaaat
     1681 tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag ttcttttaat
     1741 aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg ttccagatta
     1801 tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact aaaaactaat
     1861 tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc aaagccttta
     1921 accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc tttagctaat
     1981 acaccataag cattttccct actgatgttc atcatctgag cgtattggtt ataagtgaac
     2041 gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag tgccacacag
     2101 cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc tagttcattt
     2161 gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt ttaatcacta
     2221 taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg agttgtgggt
     2281 atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag accctctgta
     2341 aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat aatttataga
     2401 ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta taactcacta
     2461 ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa
     2521 aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggg caaatcgctg
     2581 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca
     2641 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt
     2701 atggattcat gcaaggaaac tacccataat acaagaaaag cccgtcacgg gcttctcagg
     2761 gcgttttatg gcgggtctgc tatgtggtgc tatctgactt tttgctgttc agcagttcct
     2821 gccctctgat tttccagtct gaccacttcg gattatcccg tgacaggtca ttcagactgg
     2881 ctaatgcacc cagtaaggca gcggtatcat caacaggctt acccgtctta ctgtcaacca
     2941 gacccgccag gattctcatg cataaaaaac gcccggcggc aaccgagcgt tctgaattaa
     3001 ttaatcatcg cgactgcaga gatcccagag tccttattag aagaactcgt caagaaggcg
     3061 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     3121 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     3181 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     3241 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     3301 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     3361 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     3421 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3481 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     3541 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3601 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     3661 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     3721 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     3781 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     3841 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     3901 gaaagccatc cagtttactt tgcagggctt cc
//
