LOCUS       sequence-200601-        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing PTEN , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PTEN_WT
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             163..954
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1047..1635
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1536..1555
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1789..1806
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1965..1992)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2084..2170)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2219..2241
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2233..2250
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2234..2250
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2266..2365
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3581..3680)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3697..3716)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3698..3716)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3721..3737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3721..3737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
       61 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
      121 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
      181 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
      241 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
      301 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
      361 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
      421 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
      481 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
      541 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
      601 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
      661 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
      721 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
      781 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
      841 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
      901 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
      961 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     1021 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1081 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1141 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1201 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1261 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1321 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1381 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1441 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1501 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1561 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1621 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1681 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     1741 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     1801 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1861 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     1921 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     1981 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     2041 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     2101 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     2161 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     2221 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccccaaat aatgatttta
     2281 ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt ttttataatg
     2341 ccaactttgt acaaaaaagt tggcaccatg acagccatca tcaaagagat cgttagcaga
     2401 aacaaaagga gatatcaaga ggatggattc gacttagact tgacctatat ttatccaaac
     2461 attattgcta tgggatttcc tgcagaaaga cttgaaggcg tatacaggaa caatattgat
     2521 gatgtagtaa ggtttttgga ttcaaagcat aaaaaccatt acaagatata caatctttgt
     2581 gctgaaagac attatgacac cgccaaattt aattgcagag ttgcacaata tccttttgaa
     2641 gaccataacc caccacagct agaacttatc aaaccctttt gtgaagatct tgaccaatgg
     2701 ctaagtgaag atgacaatca tgttgcagca attcactgta aagctggaaa gggacgaact
     2761 ggtgtaatga tatgtgcata tttattacat cggggcaaat ttttaaaggc acaagaggcc
     2821 ctagatttct atggggaagt aaggaccaga gacaaaaagg gagtaactat tcccagtcag
     2881 aggcgctatg tgtattatta tagctacctg ttaaagaatc atctggatta tagaccagtg
     2941 gcactgttgt ttcacaagat gatgtttgaa actattccaa tgttcagtgg cggaacttgc
     3001 aatcctcagt ttgtggtctg ccagctaaag gtgaagatat attcctccaa ttcaggaccc
     3061 acacgacggg aagacaagtt catgtacttt gagttccctc agccgttacc tgtgtgtggt
     3121 gatatcaaag tagagttctt ccacaaacag aacaagatgc taaaaaagga caaaatgttt
     3181 cacttttggg taaatacatt cttcatacca ggaccagagg aaacctcaga aaaagtagaa
     3241 aatggaagtc tatgtgatca agaaatcgat agcatttgca gtatagagcg tgcagataat
     3301 gacaaggaat atctagtact tactttaaca aaaaatgatc ttgacaaagc aaataaagac
     3361 aaagccaacc gatacttttc tccaaatttt aaggtgaagc tgtacttcac aaaaacagta
     3421 gaggagccgt caaatccaga ggctagcagt tcaacttctg taacaccaga tgttagtgac
     3481 aatgaacctg atcattatag atattctgac accactgact ctgatccaga gaatgaacct
     3541 tttgatgaag atcagcatac acaaattaca aaagtctgat tgccaacttt cttgtacaaa
     3601 gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt
     3661 caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg
     3721 gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca
     3781 caagataaaa atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact
     3841 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
     3901 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     3961 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgca
//
