LOCUS       sequence-200608-        2376 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses E. coli HPII catalase with TAG stop codon at site 283 and 
            N-terminal 6x his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Mehl R, van Zee K, Kean K
  TITLE     
  JOURNAL   2020
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "2020"
COMMENT     Sequence Label: pBad-HPII TAG283
FEATURES             Location/Qualifiers
     source          1..2376
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatcgat ggggatccga gctcgagtcg
      121 caacataacg aaaagaaccc acatcagcac cagtcaccac tacacgattc cagcgaagcg
      181 aaaccgggga tggactcact ggcacctgag gacggctctc atcgtccagc ggctgaacca
      241 acaccgccag gtgcacaacc taccgcccca gggagcctga aagcccctga tacgcgtaac
      301 gaaaaactta attctctgga agacgtacgc aaaggcagtg aaaattatgc gctgaccact
      361 aatcagggcg tgcgcatcgc cgacgatcaa aactcactgc gtgccggtag ccgtggtcca
      421 acgctgctgg aagattttat tctgcgcgag aaaatcaccc actttgacca tgagcgcatt
      481 ccggaacgta ttgttcatgc acgcggatca gccgctcacg gttatttcca gccatataaa
      541 agcttaagcg atattaccaa agcggatttc ctctcagatc cgaacaaaat caccccagta
      601 tttgtacgtt tctctaccgt tcagggtggt gctggctctg ctgataccgt gcgtgatatc
      661 cgtggctttg ccaccaagtt ctataccgaa gagggtattt ttgacctcgt tggcaataac
      721 acgccaatct tctttatcca ggatgcgcat aaattccccg attttgttca tgcggtaaaa
      781 ccagaaccgc actgggcaat tccacaaggg caaagtgccc acgatacttt ctgggattat
      841 gtttctctgc aacctgaaac tctgcacaac gtgatgtggg cgatgtcgga tcgcggcatc
      901 ccccgcagtt accgcaccat ggaaggcttc ggtattcaca ccttccgcct gattaatgcc
      961 taggggaagg caacgtttgt acgtttccac tggaaaccac tggcaggtaa agcctcactc
     1021 gtttgggatg aagcacaaaa actcaccgga cgtgacccgg acttccaccg ccgcgagttg
     1081 tgggaagcca ttgaagcagg cgattttccg gaatacgaac tgggcttcca gttgattcct
     1141 gaagaagatg aattcaagtt cgacttcgat cttctcgatc caaccaaact tatcccggaa
     1201 gaactggtgc ccgttcagcg tgtcggcaaa atggtgctca atcgcaaccc ggataacttc
     1261 tttgctgaaa acgaacaggc ggctttccat cctgggcata tcgtgccggg actggacttc
     1321 accaacgatc cgctgttgca gggacgtttg ttctcctata ccgatacaca aatcagtcgt
     1381 cttggtgggc cgaatttcca tgagattccg attaaccgtc cgacctgccc ttaccataat
     1441 ttccagcgtg acggcatgca tcgcatgggg atcgacacta acccggcgaa ttacgaaccg
     1501 aactcgatta acgataactg gccgcgcgaa acaccgccgg ggccgaaacg cggcggtttt
     1561 gaatcatacc aggagcgcgt ggaaggcaat aaagttcgcg agcgcagccc atcgtttggc
     1621 gaatattatt cccatccgcg tctgttctgg ctaagtcaga cgccatttga gcagcgccat
     1681 attgtcgatg gtttcagttt tgagttaagc aaagtcgttc gtccgtatat tcgtgagcgc
     1741 gttgttgacc agctggcgca tattgatctc actctggccc aggcggtggc gaaaaatctc
     1801 ggtatcgaac tgactgacga ccagctgaat atcaccccac ctccggacgt caacggtctg
     1861 aaaaaggatc catccttaag tttgtacgcc attcctgacg gtgatgtgaa aggtcgcgtg
     1921 gtagcgattt tacttaatga tgaagtgaga tcggcagacc ttctggccat tctcaaggcg
     1981 ctgaaggcca aaggcgttca tgccaaactg ctctactccc gaatgggtga agtgactgcg
     2041 gatgacggta cggtgttgcc tatagccgct acctttgccg gtgcaccttc gctgacggtc
     2101 gatgcggtca ttgtcccttg cggcaatatc gcggatatcg ctgacaacgg cgatgccaac
     2161 tactacctga tggaagccta caaacacctt aaaccgattg cgctggcggg tgacgcgcgc
     2221 aagtttaaag caacaatcaa gatcgctgac cagggtgaag aagggattgt ggaagctgac
     2281 agcgctgacg gtagttttat ggatgaactg ctaacgctga tggcagcaca ccgcgtgtgg
     2341 tcacgcattc ctaagattga caaaattcct gcctga
//
