LOCUS       sequence-200633-        5814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of NFS1 C381A mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Alvarez SW, Sviderskiy VO, Terzi EM, Papagiannakopoulos T, Moreira 
            AL, Adams S, Sabatini DM, Birsoy K, Possemato R
  TITLE     NFS1 undergoes positive selection in lung tumours and protects cells
            from ferroptosis.
  JOURNAL   Nature. 2017 Nov 22. doi: 10.1038/nature24637.
  PUBMED    29168506
REFERENCE   2  (bases 1 to 5814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Nov 22. doi: 10.1038/nature24637."
COMMENT     Sequence Label: pBabe hygro NFS1 CD
FEATURES             Location/Qualifiers
     source          1..5814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             98..567
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    632..831
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(2879..2899)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2884..3213
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3064..3199
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3287..4312
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             3287..4312
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     4829..4848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             5110..5579
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
      121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
      181 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      241 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      301 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      361 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      421 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      481 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      541 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
      601 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
      661 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg ccggatccac catgctgctc cgagccgctt ggaggcgggc ggcagtggcg
     1501 gtgacagcgg ctccagggcc gaagcccgcg gcgcccactc gggggctgcg cctgcgcgtt
     1561 ggagaccgtg ctcctcagtc tgcggttccc gcagatacag ccgctgcccc ggaggtgggg
     1621 ccagtgctgc gacctctcta tatggatgtg caagctacaa ctcctctgga cccccgggtg
     1681 cttgatgcca tgctccctta cctaattaac tactatggga acccacactc ccggacacat
     1741 gcttatggct gggagagtga ggcagccatg gaacgtgctc gtcagcaagt agcatctctg
     1801 attggagctg atcctcgtga gatcattttt actagtggtg ctactgaatc caacaacata
     1861 gcaattaagg gggtggcccg attctacagg tcacggaaaa agcacttgat caccacccag
     1921 acagaacaca aatgtgtctt ggactcctgc cgttcactgg aagctgaggg ctttcaggtc
     1981 acctacctcc cagtgcagaa gagtgggatc attgacctaa aggaactaga ggctgctatc
     2041 cagccagata ctagcctggt gtcagtcatg actgtgaaca atgagattgg agtgaagcag
     2101 cctattgcag aaatagggcg gatttgttca agtcgtaaag tttacttcca tactgatgca
     2161 gcccaggctg ttggaaaaat cccacttgat gtcaatgaca tgaaaattga tctcatgagc
     2221 attagtggtc acaaaatcta cggtcccaaa ggggttggtg ccatctacat ccgtcgccgg
     2281 ccccgtgtgc gtgtggaggc cctgcagagt ggaggggggc aggagcgggg tatgcggtct
     2341 gggacagtgc ccacaccctt agtggtgggg ctgggggctg cgtgtgaggt ggcacagcaa
     2401 gagatggagt atgaccacaa gcgaatctca aagttgtcag agcggctgat acagaatata
     2461 atgaagagcc ttccagatgt ggtgatgaat ggggacccta agcaccatta tcccggctgt
     2521 atcaacctct cctttgcata tgtggaaggg gaaagtctgc tgatggcact gaaggacgtt
     2581 gccttatcct cagggagtgc cgctacctct gcatccctgg agccctctta tgtgcttaga
     2641 gcaattggca ctgatgagga tttagcgcac tcttctatca ggtttggaat tggccgcttc
     2701 actacagagg aggaagtgga ctacacagtg gagaaatgca ttcagcatgt gaagcgtctt
     2761 cgagaaatga gccctctctg ggagatggtt caggatggca ttgacctcaa gagcatcaag
     2821 tggacccaac actagaagaa tggccctgag aattcgccag cacagtggtc gaccctgtgg
     2881 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     2941 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     3001 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     3061 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3121 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3181 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg ccagctgggg cgccctctgg
     3241 taaggttggg aagccctgca gatctgatcc cggggggcaa tgagatatga aaaagcctga
     3301 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     3361 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     3421 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     3481 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aatttagcga
     3541 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     3601 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     3661 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3721 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3781 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3841 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3901 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3961 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     4021 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     4081 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     4141 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     4201 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     4261 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg
     4321 ccgaccggga tctatcgatg cggcgggtgt ggtggttacg cgtaatctgc tgcttgcaaa
     4381 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4441 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     4501 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4561 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4621 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     4741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     4801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     4861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     4921 tatggaaaaa cgccagcaac gcatcgataa aataaaagat tttatttagt ctccagaaaa
     4981 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     5041 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     5101 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     5161 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     5221 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     5281 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     5341 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     5401 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     5461 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     5521 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     5581 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     5641 tagtgcggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5701 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5761 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg cccc
//
