LOCUS       sequence-200656-        9467 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Dzafic E, Strzyz PJ, Wilsch-Brauninger M, Norden C
  TITLE     Centriole Amplification in Zebrafish Affects Proliferation and 
            Survival but Not Differentiation of Neural Progenitor Cells.
  JOURNAL   Cell Rep. 2015 Oct 6;13(1):168-182. doi: 
            10.1016/j.celrep.2015.08.062. Epub 2015 Sep 24.
  PUBMED    26411683
REFERENCE   2  (bases 1 to 9467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.08.062"; journalName: "Cell Rep"; date: 
            "2015-10-6- 6"; volume: "13"; issue: "1"; pages: "168-182"
COMMENT     Sequence Label: keratin 8 (krt8)-H2B-RFP
FEATURES             Location/Qualifiers
     source          1..9467
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3579..3603
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3606..3983
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             4005..4679
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4005..4679
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4135..4153)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4403..4422
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4597..4620
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(4693..4717)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4722..4856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4746..4765)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4800..4819
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6997..7184,7185..7196)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7230..7246)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7230..7246)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7283..7303)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7283..7301)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7322..7338)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7322..7338)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7335..7357)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7346..7362
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7370..7376,7377..7394,7395..7400))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7415..7436
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7553..7570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7724..8312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7804..7823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8483..9274,9275..9343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9106..9125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9344..9448)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttgccttcc cttctacttt tgacgtcctt ttaagagctt
     1381 gtgcatgaaa gcagatttgg agctgattac tcatctcaaa cacccataca aagggatgat
     1441 tgccgtacca tgatctcaca cctttcacac ctggtttata ctatgatagt tgtagacgat
     1501 tgcgtaatgc tattaaatgc ccatcagtgc tggctgtgac acccaactgc tgccatttcg
     1561 tgttgacttg cacgagaaat gagaaattgt ctgactatgc agggtgtcta tgcgtgggaa
     1621 catttatcag tggtcattaa atactatagt ttacagttag accaaagtgt gctgtatttt
     1681 tgtgttagct tagctgcagt ttttgtgtgt gaagtaacaa atgacaaata ctcaaactat
     1741 tgtaattaag tagtttttct cagaaattgt aatttactaa gtagtttaaa aatgtgtact
     1801 tttactttcc cttgagtaca tttttagtgc agtgttggta cttttatttc acttccttcc
     1861 ttcaacctgc agtcactact ttatttattc ttgtctatgt ggattagaca aatcagtcct
     1921 gtgattcctg tccaatcaaa ttgcacatag aaggtaaatc acatcataat gaactacctt
     1981 aagacatggg ccatttataa ttgcagcaaa ctgtttgcca gcattaaaag aagatgtcaa
     2041 aaatatttac acgcattaac ccagagactg cttagatgca tgtcactgat gagaagatga
     2101 tggatgttta ctgtatgatg accgaaataa ctttaaacgc acacaagacg gcacaagacg
     2161 tcaacatggc gttaggttga cgttgtaccc caacgcagtg gggacgttgc attttgttta
     2221 gaaatgaaaa ttaggttgac gtcagaactc aacgtcaggt cgatgtcaat gttcaacatc
     2281 caatctaaaa tcatatatca atgtctaatg atgttacagc ttgatgttat gcggatgtta
     2341 cccctatgac gtctatcaga cgttggatta tggttgccat acctgatgaa taaatgtcat
     2401 tatttgacgt tggtttaaga tgttggttcg acattggatt ttggtcgctt tccaacacaa
     2461 cctaaatcca ccaaatatta acttcctatg acatcgttat tggacgtcaa aataacaata
     2521 tccttagatg ctggctagac tttgaattta ggtcaccaca acctatattt aacctaatat
     2581 taacatctta tgatgttgtg tgcctgctgg gcaataacta aatgcactac agaatgttac
     2641 gtttacacac atgtaaatta catgtaaatg catcagcttt tcacagcata atactcacta
     2701 cttactactc ttgagtactt ttaaaaaagc tacttttcac tcatactttg agtaatattt
     2761 acaactgata cttttactcg cactacattt ttaggcatgt attgatattt ttactatgat
     2821 ttttcagtac tctttccact actgcagccc tccccataca taatcgtatg tttacacata
     2881 tggtggagtt tagagccata atctacatta gctttgttag ccgctagcat tactgtgcag
     2941 aattgtgtgt gtgcacattt tccaatatca atacagaagg aaactgtgtt ccctgttccc
     3001 ttgtaaatct caacaatgca actgttcagc tcagggggaa aaatgccctg ccagatccaa
     3061 acggctggca aaagtgaatg gaaaaaagcc tttcattaat gtgaaagttg ctgcgcgccc
     3121 cacccagata aaaagagcag aggttaacat gctctctacg gctgtccagc caaccagata
     3181 ctgaggcaga aacacacccg ctggcagatg gtgagagcta cactgtcttt tccagagttt
     3241 ctactggaat gcctgtcctc aagtctcaag cctctccttg cattctctca ttccacctgg
     3301 ggcaaagccc caggctgggt gtgacaacat ttatcttacc actttctctc tgtacctgtc
     3361 taacaggtag ggtgtgtgtg agagtgcgta tgtgtgcaag tgcgtgtgtg tgtgagagca
     3421 gtcagctcca ccctctcaag agtgtgtata aaattggtca gccagctgct gagagacacg
     3481 cagagggact ttgactctcc tttgtgagca acctcctcca ctcactcctc tctcagagag
     3541 cactctcgta cctccttctc agcaactcaa agacacaggc aagtttgtac aaaaaagcag
     3601 gctggatgcc agagccagcg aagtctgctc ccgccccgaa aaagggctcc aagaaggcgg
     3661 tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg cagccgcaag gagagctatt
     3721 ccatctatgt gtacaaggtt ctgaagcagg tccaccctga caccggcatt tcgtccaagg
     3781 ccatgggcat catgaattcg tttgtgaacg acattttcga gcgcatcgca ggtgaggctt
     3841 cccgcctggc gcattacaac aagcgctcga ccatcacctc cagggagatc cagacggccg
     3901 tgcgcctgct gctgcctggg gagttggcca agcacgccgt gtccgagggt actaaggcca
     3961 tcaccaagta caccagcgct aaggatccac cgggatccac cggtatggcc tcctccgagg
     4021 acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg
     4081 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     4141 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc cctcagttcc
     4201 agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     4261 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     4321 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     4381 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     4441 ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag atgaggctga
     4501 agctgaagga cggcggccac tacgacgccg aggtcaagac cacctacatg gccaagaagc
     4561 ccgtgcagct gcccggcgcc tacaagaccg acatcaagct ggacatcacc tcccacaacg
     4621 aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgccactcc accggcgccg
     4681 tgtacatcta ggacccagct ttcttgtaca aagttgatgt agatccagac atgataagat
     4741 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4801 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4861 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat
     4921 tcgcggccgc ggcgccaatg cattgggccc tcgagcatat gagatctgtc ccgatctttc
     4981 tcttctgtgc tgtcaattta gagtagtttt tgaggtaatt tgggtgcatt ctctgtaaaa
     5041 acaacaaata aatatttata ttgtatgcaa taaatgtaac aattacacat acactctctg
     5101 attacagcat taaagggtta gttcacccaa aaatgaaaag atgtcattaa tgactcaccc
     5161 tcatgtcgtt ccaagcccgt aacacctccg ttcatcttcg gaacacagtt taagatattt
     5221 tagatttagt ccgagagctt tctgtccctc cactgaaaat gtatgtacgg tatactgtcc
     5281 atgtccagaa aggtaataaa aacatcaaag tagtccatgt gacatcagtg ggttaggtag
     5341 aattatttga agcatcgaaa atacattttg gtccaaaaat aacaaaacct acgactttat
     5401 tcagcattgt agcgtcactg cgaagtcgtg aacgcggatt gacaacagac ccggaagaga
     5461 atacaatgcc gaataaagtc gtaggttttg ttatttttgg accaaaatgt attcgatgct
     5521 tcaaaaaatt ctaactaacc cactgatgtc acatggacta ctttgatgtt tttattacct
     5581 ttctggacat ggacagtata ccgtacatac attctcaatg gaggcacaga aagctctcgg
     5641 actaaatcta aaatatctta aactgtgttc tgaagatgaa cggaggtctt acgggcttgg
     5701 aacgacatga gggcgagtca ttaatgacat tattttcatt tttgggtgaa ctaacccttt
     5761 aataaaactg acatttgcag ccaattgcac actttataat gttaaatact tgttgatttt
     5821 agaaatacag tagcatttag tgacaatcaa acattaattt tatttctgat tatattgaca
     5881 aaaacacagg actgtaaggc tgacatgaca gggtgaattg atttcattac attagttttg
     5941 atggtaacac ttttacaata aggttcgtta gttaactaca ttagttaaca tgaactaata
     6001 atgaactgca cgtatacatg ttaatttcaa catttactaa tactttatat cttgttaaca
     6061 ttagttaatg cactgtgaac taacatgaac aaacaatgaa catctgtatt tctattaact
     6121 aacgttaaca aagattaaca gatacagtaa caaatgtatt gctcatggtt atttaatgtt
     6181 agttaataca tgaactaatc aacctcattg taaagtgtta ccgttttgac aatacaacat
     6241 gtttggaaaa aaatttctcg cttggttttc ttgtgttttg catgaattca aacacaatga
     6301 gagcacggaa tgataatgat tattcaaagc tgcaataggc gccaaatttc ccgtggacct
     6361 aacgttacca attacaaatg cattttagca ttttattacg tgagccttat attgtcaatc
     6421 gcatgcatta gtgaaaatag gcctactaca catataaaag gcgcgccatc aacgtcaaaa
     6481 catgcacacc caaaaaaaaa aaaaaaaaaa aaaaaaaaaa ctatcagtaa aacaaaatac
     6541 ttaatgtact tacctcaata tgcttcctta ggtttgatgg cgaacttttg aaggccgata
     6601 tttctttatt aagcgggaga cagaggacac atttcatctt gaatgaatct ttatttacac
     6661 cacttaaaga aaagaattcg cgaagatacg gccacgggtg ctcttgatcc tgtggctgat
     6721 tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt ttcttccact
     6781 gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga
     6841 tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt catataatgt
     6901 gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca tcagcgcaat
     6961 tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac
     7021 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
     7081 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
     7141 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc
     7201 ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc
     7261 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     7321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     8041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     8101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     8161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     8281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     8341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     8401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     8461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     8521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     9001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     9061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     9121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     9181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     9241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     9301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     9361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     9421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtg
//
