LOCUS       sequence-200675-        7401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient express full length MYB with 3*FLAG tag at N terminal in 
            HEK 293T cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander 
            EL, Wilkinson JE, Challen GA, Vakoc CR
  TITLE     A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi: 
            10.1016/j.ccell.2017.12.002.
  PUBMED    29316427
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2017.12"; journalName: "Cancer Cell"; date: 
            "2018-01-8- 8"; volume: "33"; issue: "1"; pages: "13-28.e8"
COMMENT     Sequence Label: PCDNA3-N3FLAG-MYB-FL
FEATURES             Location/Qualifiers
     source          1..7401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             919..939
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             940..1005
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(2953..2971)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2954..2971)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2991..3008)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2997..3221
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3267..3695
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3354..3373)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3564..3585
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3704..3724)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3709..4039
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3890..4025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3952..3971
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4106..4900
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4160..4179)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4770..4789
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5074..5195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5111..5130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5165..5184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5244..5260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5244..5260)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5257..5279)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5268..5284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5292..5298,5299..5316,5317..5322))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5337..5358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5475..5492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5646..6234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5726..5745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6405..7196,7197..7265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7028..7047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7266..7370)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccgccatgcc aaagaagaag cgtaaggtag attacaaaga ccatgacggt
      961 gattataaag atcatgatat cgattacaag gatgacgatg acaagggagg cgaattcgcc
     1021 cggagacccc gacacagcat ctacagtagc gatgaagatg atgaagacat tgagatgtgt
     1081 gaccatgact acgatgggct gctgcccaaa tctggaaagc gtcacttggg gaaaactagg
     1141 tggacaaggg aagaggatga gaagctgaag aagctggtgg aacagaacgg aacagacgac
     1201 tggaaagtca ttgccaatta tctgcccaac cggacagatg tgcagtgcca acaccggtgg
     1261 cagaaagtgc tgaaccctga actcatcaaa ggtccctgga ccaaagaaga agatcagaga
     1321 gtcatagagc ttgtccagaa atatggtccg aagcgttggt ctgttattgc caagcactta
     1381 aaagggagaa ttggaaagca gtgtcgggag aggtggcaca accatttgaa tccagaagtt
     1441 aagaaaacct cctggacaga agaggaggac agaatcattt accaggcaca caagcgtctg
     1501 gggaacagat gggcagagat cgcaaagctg ctgcccggac ggactgataa tgctatcaag
     1561 aaccactgga attccaccat gcgtcgcaag gtggaacagg aaggctacct gcaggagcct
     1621 tccaaagcca gccagacgcc agtggccacg agcttccaga agaacaatca tttgatgggg
     1681 tttgggcatg cctcacctcc atctcagctc tctccaagtg gccagtcctc cgtcaacagc
     1741 gaatatccct attaccacat cgccgaagca caaaacatct ccagtcacgt tccctatcct
     1801 gtcgcattgc atgttaatat agtcaacgtc cctcagccgg ctgcggcagc catccagaga
     1861 cactataacg acgaagaccc tgagaaggaa aagcgaataa aggagctgga gttgctcctg
     1921 atgtcaacag agaacgagct gaagggacag caggcattac caacacagaa ccacacttgc
     1981 agctaccccg ggtggcacag cacctccatt gtggaccaga ccagacctca tggggatagt
     2041 gcacctgttt cctgtttggg agaacaccat gccaccccat ctctgcctgc agatcccggc
     2101 tccctacctg aagaaagtgc ctcaccagca aggtgcatga tcgtccacca gggcaccatt
     2161 ctggacaatg ttaagaacct cttagaattt gcagaaacac tccagtttat agattctttc
     2221 ttgaacactt ccagcaacca tgaaagctcg ggcttagatg cacctacctt accctccact
     2281 cctctcattg gtcacaaact gacaccatgt cgagaccaga ctgtgaaaac ccagaaggaa
     2341 aattccatct ttagaactcc agctatcaaa aggtcaatcc tcgaaagctc tcctcgaact
     2401 cccacaccat tcaaacatgc ccttgcagct caagaaatta aatacggtcc cctgaagatg
     2461 ctacctcaga ccccctccca tgcagtggag gacctacaag atgtgattaa gcaggaatcg
     2521 gatgaatctg gaattgtggc tgagtttcaa gagagtggac caccgttact gaaaaaaatc
     2581 aagcaggagg tggagtcgcc aactgagaaa tcgggaaact tcttctgctc aaaccactgg
     2641 gcagagaaca gcctgagcac ccagctgttc tcgcaggcgt ctcctgtggc agatgcccca
     2701 aatattctta caagctctgt tttaatgaca cctgtatcag aagatgaaga caatgtcctc
     2761 aaagccttta ccgtacctaa gaacaggccc ctggtgggtc ccttgcagcc atgcagtggt
     2821 gcctgggagc cagcatcctg tgggaagaca gaggaccaga tgacggcctc cggtccggct
     2881 cggaaatacg tgaacgcgtt ctcagctcga actctggtca tgtgactcga gcatgcatct
     2941 agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt
     3001 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     3061 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     3121 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     3181 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     3241 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     3301 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     3361 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3421 gggcatccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3481 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3541 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     3601 tatctcggtc tattcttttg atttataagg gattttgggg atttcggcct attggttaaa
     3661 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     3721 gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca tgcatctcaa
     3781 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3841 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3901 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3961 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4021 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4081 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4141 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4201 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4261 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4321 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4381 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4441 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4501 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4561 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4621 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4681 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4741 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4801 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4861 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     4921 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     4981 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5041 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5101 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5161 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     5221 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5281 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5341 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5401 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5461 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5521 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5581 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5641 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5701 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5761 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5821 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5881 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5941 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6001 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6061 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     6121 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6181 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     6241 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     6301 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     6361 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     6421 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     6481 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6541 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6601 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6661 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6721 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6781 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6841 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6901 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6961 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     7021 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     7081 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     7141 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     7201 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     7261 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     7321 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     7381 cgaaaagtgc cacctgacgt c
//
