LOCUS       sequence-200688-       10728 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse Fzd3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10728)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307-mFzd3
FEATURES             Location/Qualifiers
     source          1..10728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        93..1271
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          324..1262
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1219..1239
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1291..1315
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3410..3544
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3634..3658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3666..3707
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3758..4346
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3811..3831)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4229..4240)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4418..4651
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4729..4850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4890..5025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5046..5064)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5074..5091)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5074..5090)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5083..5105)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5232..5687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5319..5338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5529..5550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5713..5817
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5818..5886,5887..6678)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6036..6055)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6849..7437
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7338..7357
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7591..7608
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7725..7746
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7761..7766,7767..7784,7785..7791)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7799..7815
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7804..7826
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7823..7839
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7823..7839
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7858..7878
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7860..7878
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7906..8132
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8133..8313
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8360..8485
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8978..9211
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9396..9440
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9671..9881
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9693..9889
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9740..9875
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9921..10523
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9921..9940)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10417..10437
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10566..10683
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gaggcctgca ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat
       61 ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc
      121 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
      181 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      241 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      301 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
      361 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
      421 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
      481 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
      541 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
      601 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
      661 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
      721 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
      781 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
      841 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
      901 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
      961 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     1021 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     1081 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     1141 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     1201 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     1261 aggtgtcgtg aggctagcat cgattgatca acaagtttgt acaaaaaagc aggctggcgc
     1321 cggaaccaat tcagtcgact ggatccggta ccgaattcag gccggcctac catggctgtg
     1381 agctggattg tctttgatct ttggctcttg actgtgtttc tggggcagat aggtgggcac
     1441 agtttgtttt cttgtgaacc tataaccttg aggatgtgcc aagatttgcc ttacaatact
     1501 accttcatgc ctaatcttct gaaccattat gaccaacaga ctgcagcttt agcaatggag
     1561 cccttccacc ctatggtgaa cctggattgt tctcgggatt ttcggccatt tctttgtgca
     1621 ctctatgccc ctatttgtat ggaatatgga cgtgtcacac ttccctgccg taggctgtgt
     1681 cagcgtgcct atagcgagtg ttcaaaactc atggagatgt ttggtgtccc gtggcctgaa
     1741 gatatggagt gcagtaggtt tccagattgt gatgagccat atccccgact tgtggatttg
     1801 aatttagttg gagatccaac tgaaggagcc ccagttgcag tgcagaggga ctatggtttt
     1861 tggtgtccca gagagttaaa aattgatcct gatcttggct attcctttct gcacgtgcga
     1921 gattgttcgc caccatgtcc caatatgtac ttcaggagag aagaactgtc atttgctcgc
     1981 tatttcatag gcctgatttc aatcatttgc ctctctgcca cattgtttac ttttttaacc
     2041 tttctaattg acgtcacaag attccgttac cctgaaagac ctatcatatt ttatgcagtc
     2101 tgctacatga tggtgtcatt aattttcttc attgggtttt tgctggagga ccgagtagcc
     2161 tgcaatgcat ctagccctgc acagtataag gcttctacag tgacacaagg atctcacaat
     2221 aaggcctgta ccatgctctt tatggtacta tattttttca ctatggctgg cagtgtatgg
     2281 tgggtaattc ttaccatcac atggttttta gcagctgtgc caaagtgggg cagtgaagct
     2341 attgagaaga aagcattgct gtttcatgcc agtgcctggg gcatccccgg aactctaact
     2401 atcatccttt tagcgatgaa taaaattgaa ggtgacaata ttagtggcgt gtgttttgtc
     2461 ggcctctacg acgttgatgc attaagatat ttcgttctcg ctcccctctg cctgtatgtg
     2521 gtagttgggg tttctctcct tttagccggc attatatccc taaacagagt tcggattgag
     2581 atcccattag aaaaggaaaa ccaagataag ttagtgaagt tcatgatccg gattggtgtt
     2641 ttcagcattc tctaccttgt gccactcttg gttgtaattg gatgttactt ttatgagcaa
     2701 gcttaccgcg gcatctggga gacaacatgg atccaggaac gctgcagaga gtatcacatt
     2761 ccatgtccgt accaggttac tcagatgagt cgtccagacc tgattctctt tctgatgaag
     2821 tatctcatgg ctctcatagt tgggattccc tctatatttt gggttggaag caaaaagaca
     2881 tgctttgaat gggccagttt tttccatggg cgtaggaaaa aagagatagt gaatgagagc
     2941 cggcaggtgc tccaggaacc tgactttgct cagtcactcc tgagggaccc aaatactcca
     3001 attataagaa aatcaagagg aacttccact caagggacat ccacacatgc ttcttcaact
     3061 cagctggcca tggtggatga ccaaagaagc aaagcaggga gtgtccacag caaagtgagc
     3121 agctaccatg gcagcctcca caggtcacgg gatggcaggt acactccctg cagttaccga
     3181 ggaatggagg agagactacc tcacggcagc atgtcacggc tgacggatca ttccaggcac
     3241 agtagttctc atcggctcaa cgagcagtcc cgacacagca gcatccgaga cctcagtaac
     3301 aaccccatga ctcacattac acatggcacc agcatgaacc gtgttattga ggaggatgga
     3361 accagtgctt agggcgcgcc tctagaacta tagtgagtcg tattacgtag atccagacat
     3421 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3481 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3541 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     3601 tttttaattc gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggttg
     3661 atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgaacta
     3721 gtaccggtta agtcgacaat caacgcgtta agtcgacaat caacctctgg attacaaaat
     3781 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3841 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3901 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3961 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4021 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4081 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     4141 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     4201 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     4261 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     4321 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     4381 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4441 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4501 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4561 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4621 cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt
     4681 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     4741 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     4801 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     4861 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4921 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4981 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     5041 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     5101 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     5161 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     5221 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5521 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5581 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5641 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     5701 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5761 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     5821 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5881 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5941 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     6001 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     6061 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     6121 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     6181 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     6241 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     6301 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     6361 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     6421 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     6481 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     6541 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     6601 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     6661 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     6721 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     6781 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6841 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6901 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6961 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     7021 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     7081 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     7141 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     7201 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     7261 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     7321 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     7381 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     7441 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     7501 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     7561 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     7621 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     7681 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     7741 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     7801 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     7861 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
     7921 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
     7981 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
     8041 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
     8101 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
     8161 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8221 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8281 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     8341 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     8401 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     8461 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     8521 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     8581 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     8641 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     8701 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     8761 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     8821 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     8881 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     8941 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     9001 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     9061 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     9121 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     9181 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     9241 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     9301 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     9361 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     9421 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     9481 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     9541 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     9601 ccccgagggg acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     9661 aggctcccca gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt
     9721 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9781 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9841 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag
     9901 aggtaccacc ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     9961 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
    10021 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
    10081 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg
    10141 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
    10201 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
    10261 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
    10321 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
    10381 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
    10441 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
    10501 cccgcaagcc cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc
    10561 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
    10621 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
    10681 tttcgggttt attacaggga cagcagagat ccactttggc gccggctc
//
