LOCUS       sequence-200700-       12533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of mCherry-LacR-BRCA1 siRNA resistant 
            construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12533)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 12533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pDEST-mCherry-LacR-BRCA1
FEATURES             Location/Qualifiers
     source          1..12533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             984..1691
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1129..1147)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1397..1416
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(1735..1754)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             2796..2816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    2826..2850
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8447..8471)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8513..8530)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8519..8743
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8876..8895)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    9027..9074
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9082..10104
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGDFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10234..10355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10271..10290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10325..10344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10404..10420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10404..10420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10417..10439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10428..10444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10452..10458,10459..10476,10477..10482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10497..10518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10635..10652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10806..11394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10886..10905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11565..12356,12357..12425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12188..12207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12426..12530)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttatggtga gcaagggcga ggaggataac atggccatca
     1021 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     1081 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     1141 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     1201 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     1261 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     1321 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     1381 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     1441 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     1501 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     1561 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     1621 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     1681 agctgtacaa gtccggactc agatctcgag ggatggtgaa accagtaacg ttatacgatg
     1741 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc
     1801 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc
     1861 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct
     1921 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc
     1981 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag
     2041 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg
     2101 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg
     2161 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac
     2221 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat
     2281 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc
     2341 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa
     2401 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga
     2461 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct
     2521 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca
     2581 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc
     2641 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca
     2701 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2761 tggcacgaca ggtttcccga agcagcctga ggcctcctaa gaagaagagg aaggttggcg
     2821 cgcccacaag tttgtacaaa aaagcaggct tcaccatgga tttatctgct cttcgcgttg
     2881 aagaagtaca aaatgtcatt aatgctatgc agaaaatctt agagtgtccc atctgtctgg
     2941 agttgatcaa ggaacctgtc tccacaaagt gtgaccacat attttgcaaa ttttgcatgc
     3001 tgaaacttct caaccagaag aaagggcctt cacagtgtcc tttatgtaag aatgatataa
     3061 ccaaaaggag cctacaagaa agtacgagat ttagtcaact tgttgaagag ctattgaaaa
     3121 tcatttgtgc ttttcagctt gacacaggtt tggagtatgc aaacagctat aattttgcaa
     3181 aaaaggaaaa taactctcct gaacatctaa aagatgaagt ttctatcatc caaagtatgg
     3241 gctacagaaa ccgtgccaaa agacttctac agagtgaacc cgaaaatcct tccttgcagg
     3301 aaaccagtct cagtgtccaa ctctctaacc ttggaactgt gagaactctg aggacaaagc
     3361 agcggataca acctcaaaag acgtctgtct acattgaatt gggatctgat tcttctgaag
     3421 ataccgttaa taaggcaact tattgcagtg tgggagatca agaattgtta caaatcaccc
     3481 ctcaaggaac cagggatgaa atcagtttgg attctgcaaa aaaggctgct tgtgaatttt
     3541 ctgagacgga tgtaacaaat actgaacatc atcaacccag taataatgat ttgaacacca
     3601 ctgagaagcg tgcagctgag aggcatccag aaaagtatca gggtagttct gtttcaaact
     3661 tgcatgtgga gccatgtggc acaaatactc atgccagctc attacagcat gagaacagca
     3721 gtttattact cactaaagac agaatgaatg tagaaaaggc tgaattctgt aataaaagca
     3781 aacagcctgg cttagcaagg agccaacata acagatgggc tggaagtaag gaaacatgta
     3841 atgataggcg gactcccagc acagaaaaaa aggtagatct gaatgctgat cccctgtgtg
     3901 agagaaaaga atggaataag cagaaactgc catgctcaga gaatcctaga gatactgaag
     3961 atgttccttg gataacacta aatagcagca ttcagaaagt taatgagtgg ttttccagaa
     4021 gtgatgaact gttaggttct gatgactcac atgatgggga gtctgaatca aatgccaaag
     4081 tagctgatgt attggacgtt ctaaatgagg tagatgaata ttctggttct tcagagaaaa
     4141 tagacttact ggccagtgat cctcatgagg ctttaatatg taaaagtgaa agagttcact
     4201 ccaaatcagt agagagtaat attgaagaca aaatatttgg gaaaacctat cggaagaagg
     4261 caagcctccc caacttaagc catgtaactg aaaatctaat tataggagca tttgttactg
     4321 agccacagat aatacaagag cgtcccctca caaataaatt aaagcgtaaa aggagaccta
     4381 catcaggcct tcatcctgag gattttatca agaaagcaga tttggcagtt caaaagactc
     4441 ctgaaatgat aaatcaggga actaaccaaa cggagcagaa tggtcaagtg atgaatatta
     4501 ctaatagtgg tcatgagaat aaaacaaaag gtgattctat tcagaatgag aaaaatccta
     4561 acccaataga atcactcgaa aaagaatctg ctttcaaaac gaaagctgaa cctataagca
     4621 gcagtataag caatatggaa ctcgaattaa atatccacaa ttcaaaagca cctaaaaaga
     4681 ataggctgag gaggaagtct tctaccaggc atattcatgc gcttgaacta gtagtcagta
     4741 gaaatctaag cccacctaat tgtactgaat tgcaaattga tagttgttct agcagtgaag
     4801 agataaagaa aaaaaagtac aaccaaatgc cagtcaggca cagcagaaac ctacaactca
     4861 tggaaggtaa agaacctgca actggagcca agaagagtaa caagccaaat gaacagacaa
     4921 gtaaaagaca tgacagcgat actttcccag agctgaagtt aacaaatgca cctggttctt
     4981 ttactaagtg ttcaaatacc agtgaactta aagaatttgt caatcctagc cttccaagag
     5041 aagaaaaaga agagaaacta gaaacagtta aagtgtctaa taatgctgaa gaccccaaag
     5101 atctcatgtt aagtggagaa agggttttgc aaactgaaag atctgtagag agtagcagta
     5161 tttcattggt acctggtact gattatggca ctcaggaaag tatctcgtta ctggaagtta
     5221 gcactctagg gaaggcaaaa acagaaccaa ataaatgtgt gagtcagtgt gcagcatttg
     5281 aaaaccccaa gggactaatt catggttgtt ccaaagataa tagaaatgac acagaaggct
     5341 ttaagtatcc attgggacat gaagttaacc acagtcggga aacaagcata gaaatggaag
     5401 aaagtgaact tgatgctcag tatttgcaga atacattcaa ggtttcaaag cgccagtcat
     5461 ttgctccgtt ttcaaatcca ggaaatgcag aagaggaatg tgcaacattc tctgcccact
     5521 ctgggtcctt aaagaaacaa agtccaaaag tcacttttga atgtgaacaa aaggaagaaa
     5581 atcaaggaaa gaatgagtct aatatcaagc ctgtacagac agttaatatc actgcaggct
     5641 ttcctgtggt tggtcagaaa gataagccag ttgataatgc caaatgtagt atcaaaggag
     5701 gctctaggtt ttgtctatca tctcagttca gaggcaacga aactggactc attactccaa
     5761 ataaacatgg acttttacaa aacccatatc gtataccacc actttttccc atcaagtcat
     5821 ttgttaaaac taaatgtaag aaaaatctgc tagaggaaaa ctttgaggaa cattcaatgt
     5881 cacctgaaag agaaatggga aatgagaaca ttccaagtac agtgagcaca attagccgta
     5941 ataacattag agaaaatgtt tttaaagaag ccagctcaag caatattaat gaagtaggtt
     6001 ccagtactaa tgaagtgggc tccagtatta atgaaatagg ttccagtgat gaaaacattc
     6061 aagcagaact aggtagaaac agagggccaa aattgaatgc tatgcttaga ttaggggttt
     6121 tgcaacctga ggtctataaa caaagtcttc ctggaagtaa ttgtaagcat cctgaaataa
     6181 aaaagcaaga atatgaagaa gtagttcaga ctgttaatac agatttctct ccatatctga
     6241 tttcagataa cttagaacag cctatgggaa gtagtcatgc atctcaggtt tgttctgaga
     6301 cacctgatga cctgttagat gatggtgaaa taaaggaaga tactagtttt gctgaaaatg
     6361 acattaagga aagttctgct gtttttagca aaagcgtcca gaaaggagag cttagcagga
     6421 gtcctagccc tttcacccat acacatttgg ctcagggtta ccgaagaggg gccaagaaat
     6481 tagagtcctc agaagagaac ttatctagtg aggatgaaga gcttccctgc ttccaacact
     6541 tgttatttgg taaagtaaac aatatacctt ctcagtctac taggcatagc accgttgcta
     6601 ccgagtgtct gtctaagaac acagaggaga atttattatc attgaagaat agcttaaatg
     6661 actgcagtaa ccaggtaata ttggcaaagg catctcagga acatcacctt agtgaggaaa
     6721 caaaatgttc tgctagcttg ttttcttcac agtgcagtga attggaagac ttgactgcaa
     6781 atacaaacac ccaggatcct ttcttgattg gttcttccaa acaaatgagg catcagtctg
     6841 aaagccaggg agttggtctg agtgacaagg aattggtttc agatgatgaa gaaagaggaa
     6901 cgggcttgga agaaaataat caagaagagc aaagcatgga ttcaaactta ggtgaagcag
     6961 catctgggtg tgagagtgaa acaagcgtct ctgaagactg ctcagggcta tcctctcaga
     7021 gtgacatttt aaccactcag cagagggata ccatgcaaca taacctgata aagctccagc
     7081 aggaaatggc tgaactagaa gctgtgttag aacagcatgg gagccagcct tctaacagct
     7141 acccttccat cataagtgac tcttctgccc ttgaggacct gcgaaatcca gaacaaagca
     7201 catcagaaaa agcagtatta acttcacaga aaagtagtga ataccctata agccagaatc
     7261 cagaaggcct ttctgctgac aagtttgagg tgtctgcaga tagttctacc agtaaaaata
     7321 aagaaccagg agtggaaagg tcatcccctt ctaaatgccc atcattagat gataggtggt
     7381 acatgcacag ttgctctggg agtcttcaga atagaaacta cccatctcaa gaggagctca
     7441 ttaaggttgt tgatgtggag gagcaacagc tggaagagtc tgggccacac gatttgacgg
     7501 aaacatctta cttgccaagg caagatctag agggaacccc ttacctggaa tctggaatca
     7561 gcctcttctc tgatgaccct gaatctgatc cttctgaaga cagagcccca gagtcagctc
     7621 gtgttggcaa cataccatct tcaacctctg cattgaaagt tccccaattg aaagttgcag
     7681 aatctgccca gagtccagct gctgctcata ctactgatac tgctgggtat aatgcaatgg
     7741 aagaaagtgt gagcagggag aagccagaat tgacagcttc aacagaaagg gtcaacaaaa
     7801 gaatgtccat ggtggtgtct ggcctgaccc cagaagaatt tatgctcgtg tacaagtttg
     7861 ccagaaaaca ccacatcact ttaactaatc taattactga agagactact catgttgtta
     7921 tgaaaacaga tgctgagttt gtgtgtgaac ggacactgaa atattttcta ggaattgcgg
     7981 gaggaaaatg ggtagttagc tatttctggg tgacccagtc tattaaagaa agaaaaatgc
     8041 tgaatgagca tgattttgaa gtcagaggag atgtggtcaa tggaagaaac caccaaggtc
     8101 caaagcgagc aagagaatcc caggacagaa agatcttcag ggggctagaa atctgttgct
     8161 atgggccctt caccaacatg cccacagatc aactggaatg gatggtacag ctgtgtggtg
     8221 cttctgtggt gaaggagctt tcatcattca cccttggcac aggtgtccac ccaattgtgg
     8281 ttgtgcagcc agatgcctgg acagaggaca atggcttcca tgcaattggg cagatgtgtg
     8341 aggcacctgt ggtgacccga gagtgggtgt tggacagtgt agcactctac cagtgccagg
     8401 agctggacac ctacctgata ccccagatcc cccacagcca ctactgaatc agctttcttg
     8461 tacaaagtgg tgtgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     8521 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8581 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8641 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8701 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8761 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8821 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8881 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8941 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9001 gattagggtg atggttcacg tacctagaag ttcctattcc gaagttccta ttctctagaa
     9061 agtataggaa cttccttggc caaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     9121 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     9181 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     9241 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     9301 ccggaagtgc ttgacattgg ggatttcagc gagagcctga cctattgcat ctcccgccgt
     9361 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     9421 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     9481 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     9541 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     9601 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     9661 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     9721 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     9781 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     9841 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     9901 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     9961 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
    10021 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
    10081 actcgtccga gggcaaagga atagcacgta ctacgagatt tcgattccac cgccgccttc
    10141 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    10201 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
    10261 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    10321 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
    10381 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    10441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    10501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10561 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10621 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    10681 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10741 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10801 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10861 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10921 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10981 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    11041 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    11101 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    11161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    11221 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    11281 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    11341 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    11401 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    11461 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    11521 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    11581 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    11641 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11701 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11761 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11821 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11881 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11941 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    12001 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    12061 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    12121 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    12181 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    12241 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    12301 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    12361 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    12421 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    12481 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cac
//
