LOCUS       sequence-200704-        6466 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  heat shock inducible transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C
  TITLE     Interkinetic nuclear migration is centrosome independent and ensures
            apical cell division to maintain tissue integrity.
  JOURNAL   Dev Cell. 2015 Jan 26;32(2):203-19. doi: 
            10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15.
  PUBMED    25600237
REFERENCE   2  (bases 1 to 6466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.12.001"; journalName: "Dev Cell"; date: 
            "2015-01-26- 26"; volume: "32"; issue: "2"; pages: "203-19"
COMMENT     Sequence Label: HS-DN-Plk1
FEATURES             Location/Qualifiers
     source          1..6466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2945..3079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2969..2988)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3023..3042
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3192..3212)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3192..3210)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3231..3247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3231..3247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3244..3266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3255..3271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3279..3285,3286..3303,3304..3309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3324..3345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3462..3479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3633..4221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3713..3732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4392..5183,5184..5252))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5015..5034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5253..5357)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5383..5838)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5520..5541)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5732..5751
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcaggggtgt cgcttggtta tttccaaaaa tcaaattaat tttattaaac tattagaacg
       61 agcatgtttt gtctatatgc tacagaagat aaaaaataat aggagttaac agttataaaa
      121 caacacactt tgtttctatt gattgttgac cacactgggg tctcattaag ttagattaaa
      181 gacacactaa ctgggtcaaa agcagcagat tgatttcata gcaccagggt aaactttcta
      241 acacttttac ggcaatcata tacattaaaa ttaaatacag accacgactg aacaaggagg
      301 atgatctcca atattaaaca aagagacttg tgcctatttc tctgagggta aacatgacct
      361 ctcaagttag caagttgttt ttaacactac aaaaatagtt aagactgcaa tcccagaata
      421 aagtattggt tttaaccaat caatatagta cagtaaacat ccatttgttt tgttgaaacg
      481 ttaaacaaat ctgaccaaag ctattagctt atataaaaca ggtttgcctt ctatgtagct
      541 gaaaacacca caggcccgat tttgctactg tgtaaaacat ttcagcaaga tttttttatt
      601 gcattttttt tactgaatcn tcaaacattt tatcatttta gtttgttcat tcattgcaac
      661 tggaaaaaca acacatcaca caaccgcaca tatttcagca ataagtacaa taaaacactc
      721 aaataaaaaa aacattttaa atctctttgt atttttgacc gctgtttcgc gtaatttcac
      781 ggtaaaactc tggaaatctc cactacattc ctctcagcgg ctcctctcaa tgacagctga
      841 agaagtgacg cggctgcctg ctgtgttttg attggtcgaa ttcactggag gcttccagaa
      901 cagtgtagag tctgaacggg tgcgcgctct gctgtattta aagggcgaaa gagagaccgc
      961 agagaaactc aaccgaagag aagcgacttg acaaagaaga aaagagcagc ctgacaggac
     1021 tttttccccg acgaggtgtt tattcgctct atttaagaat ctactgtaag gtaagtctca
     1081 atatattgta ctctattggc taatcagaat tatatagaga ttatatgtac ttaatgtcaa
     1141 aaaatcaact ttgtatatgt aatcttttta catgtggact gcctatgttc atcttatttt
     1201 aggtctacta gaaaattata tttcccgttt tcacaataag gatttttaaa aaaagcaatg
     1261 aacagacggg catttacttt atgttgctga cattatttta tatgagcata ataaccataa
     1321 atactagcaa atgtcctaaa tgaatttgtg ttaatgttgt ctacaaaaga aaattagcgt
     1381 tttacttgta caactaataa taacttggtt attaagagaa tttcacttgt tgactagaaa
     1441 aatcctttca taatgaaaca attgcaccat aaattgtata aatataaaat taattctaat
     1501 tgtttttttt taagcttctc ctggagctgc acaagaggag gaaagccctg actgagcctg
     1561 aggcccgata ctacctacgg caaattgtgc ttggctgcca gtacctgcac cgaaaccgag
     1621 ttattcatcg agacctcaag ctgggcaacc ttttcctgaa tgaagatctg gaggtgaaaa
     1681 taggggattt tggactggca accaaagtcg aatatgacgg ggagaggaag aagaccctgt
     1741 gtgggactcc taattacata gctcccgagg tgctgagcaa gaaagggcac agtttcgagg
     1801 tggatgtgtg gtccattggg tgtatcatgt ataccttgtt agtgggcaaa ccaccttttg
     1861 agacttcttg cctaaaagag acctacctcc ggatcaagaa gaatgaatac agtattccca
     1921 agcacatcaa ccccgtggcc gcctccctca tccagaagat gcttcagaca gatcccactg
     1981 cccgcccaac cattaacgag ctgcttaatg acgagttctt tacttctggc tatatccctg
     2041 cccgtctccc catcacctgc ctgaccattc caccaaggtt ttcgattgct cccagcagcc
     2101 tggaccccag caaccggaag cccctcacag tcctcaataa aggcttggag aaccccctgc
     2161 ctgagcgtcc ccgggaaaaa gaagaaccag tggttcgaga gacaggtgag gtggtcgact
     2221 gccacctcag tgacatgctg cagcagctgc acagtgtcaa tgcctccaag ccctcggagc
     2281 gtgggctggt caggcaagag gaggctgagg atcctgcctg catccccatc ttctgggtca
     2341 gcaagtgggt ggactattcg gacaagtacg gccttgggta tcagctctgt gataacagcg
     2401 tgggggtgct cttcaatgac tcaacacgcc tcatcctcta caatgatggt gacagcctgc
     2461 agtacataga gcgtgacggc actgagtcct acctcaccgt gagttcccat cccaactcct
     2521 tgatgaagaa gatcaccctc cttaaatatt tccgcaatta catgagcgag cacttgctga
     2581 aggcaggtgc caacatcacg ccgcgcgaag gtgatgagct cgcccggctg ccctacctac
     2641 ggacctggtt ccgcacccgc agcgccatca tcctgcacct cagcaacggc agcgtgcaga
     2701 tcaacttctt ccaggatcac accaagctca tcttgtgccc actgatggca gccgtgacct
     2761 acatcgacga gaagcgggac ttccgcacat accgcctgag tctcctggag gagtacggct
     2821 gctgcaagga gctggccagc cggctccgct acgcccgcac tatggtggac aagctgctga
     2881 gctcacgctc ggccagcaac cgtctcaagg cctcctctag aactatagtg agtcgtatta
     2941 cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     3001 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     3061 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     3121 ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc
     3181 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     3241 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3301 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3361 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3421 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3481 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3541 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3601 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3661 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3721 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3781 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3841 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3901 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3961 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4021 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     4081 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4141 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4201 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4261 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4321 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4381 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4441 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4501 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4561 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4621 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4681 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4741 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4801 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4861 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4921 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4981 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5041 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5101 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5161 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5221 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5281 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5341 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     5401 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     5461 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     5521 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     5581 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     5641 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     5701 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     5761 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     5821 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     5881 cggtgcgggc ctcttcgcta ttacgccagt cgaccatagc caattcaata tggcgtatat
     5941 ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata tggcgtatat
     6001 ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata tggcggactt
     6061 ggcaccatgc caattcaata tggcggactt ggcactgtgc caactgggga ggggtctact
     6121 tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc catattgaat
     6181 tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat atataggcaa
     6241 tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta tgtattggcc
     6301 ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc ccctcccaat
     6361 gggcggtccc atataccata tatggggctt cctaataccg cccatagcca ctcccccatt
     6421 gacgtcaatg gtctctatat atggtctttc ctattgacgt catatg
//
