LOCUS       sequence-200733-        9236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human GART-GFP fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9236)
  AUTHORS   An S, Kumar R, Sheets ED, Benkovic SJ
  TITLE     Reversible compartmentalization of de novo purine biosynthetic 
            complexes in living cells.
  JOURNAL   Science. 2008 Apr 4;320(5872):103-6. doi: 10.1126/science.1152241.
  PUBMED    18388293
REFERENCE   2  (bases 1 to 9236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2008-04-4- 4"; volume: "320"; issue: 
            "5872"; pages: "103-6"
COMMENT     Sequence Label: pGART-GFP
FEATURES             Location/Qualifiers
     source          1..9236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4148..4861
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(4178..4206)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4801..4821
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    4957..5078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4968..4987)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5022..5041
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5264..5719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5351..5370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5561..5582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5836..6193
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5836..6027
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5859..5879)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6044..6179
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6298..6317)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6891..6910
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7085..7133
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7174..7193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7293..7315
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7353..7371)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7439..7543
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7544..7612,7613..8404)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7762..7781)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8575..9163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9064..9083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc atggctgctc gtgtacttat aattggcagt ggaggaaggg aacatacgct
     1141 ggcctggaaa cttgcacagt ctcatcatgt caaacaagtg ttggttgccc caggaaacgc
     1201 aggcactgcc tgctctgaaa agatttcaaa taccgccatc tcaatcagtg accacactgc
     1261 ccttgctcaa ttctgcaaag agaagaaaat tgaatttgta gttgttggac cagaagcacc
     1321 tctggctgct gggattgttg ggaacctgag gtctgcagga gtgcaatgct ttggcccaac
     1381 agcagaagcg gctcagttag agtccagcaa aaggtttgcc aaagagttta tggacagaca
     1441 tggaatccca accgcacaat ggaaggcttt caccaaacct gaagaagcct gcagcttcat
     1501 tttgagtgca gacttccctg ctttggttgt gaaggccagt ggtcttgcag ctggaaaagg
     1561 ggtgattgtt gcaaagagca aagaagaggc ctgcaaagct gtacaagaga tcatgcagga
     1621 gaaagccttt ggggcagctg gagaaacaat tgtcattgaa gaacttcttg acggagaaga
     1681 ggtgtcgtgt ctgtgtttca ctgatggcaa gactgtggcc cccatgcccc cagcacagga
     1741 ccataagcga ttactggagg gagatggtgg ccctaacaca gggggaatgg gagcctattg
     1801 tccagcccct caggtttcta atgatctatt actaaaaatt aaagatactg ttcttcagag
     1861 gacagtggat ggcatgcagc aagagggtac tccatataca ggtattctct atgctggaat
     1921 aatgctgacc aagaatggcc caaaagttct agagtttaat tgccgttttg gtgatccaga
     1981 gtgccaagta atcctcccac ttcttaaaag tgacctttat gaagtgattc agtccacctt
     2041 agatggactg ctctgcacat ctctgcctgt ttggctagaa aaccacaccg ccctaactgt
     2101 tgtcatggca agtaaaggtt atcctggaga ctacaccaag ggtgtagaga taacagggtt
     2161 tcctgaggct caagctctag gactggaggt gttccatgca ggcactgccc tcaaaaatgg
     2221 caaagtagta actcatgggg gtagagttct tgcagtcaca gccatccggg aaaatctcat
     2281 atcagccctt gaggaagcca agaaaggact agctgctata aagtttgagg gagcaattta
     2341 taggaaagac gtcggctttc gtgccatagc tttcctccag cagcccagga gtttgactta
     2401 caaggaatct ggagtagata tcgcagctgg aaatatgctg gtcaagaaaa ttcagccttt
     2461 agcaaaagcc acttccagat caggctgtaa agttgatctt ggaggttttg ctggtctttt
     2521 tgatttaaaa gcagctggtt tcaaagatcc ccttctggcc tctggaacag atggcgttgg
     2581 aactaaacta aagattgccc agctatgcaa taaacatgat accattggtc aagatttggt
     2641 agcaatgtgt gttaatgata ttctggcaca aggagcagag cccctcttct tccttgatta
     2701 cttttcctgt ggaaaacttg acctcagtgt aactgaagct gttgttgctg gaattgctaa
     2761 agcttgtgga aaagctggat gtgctctcct tggaggtgaa acagcagaaa tgcctgacat
     2821 gtatccccct ggagagtatg acctagctgg gtttgccgtt ggtgccatgg agcgagatca
     2881 gaaactccct cacctggaaa gaatcactga gggtgatgtt gttgttggaa tagcttcatc
     2941 tggtcttcat agcaatggat ttagccttgt gaggaaaatc gtggcaaaat cttccctcca
     3001 gtactcctct ccagcacctg atggttgtgg tgaccagact ttaggggact tacttctcac
     3061 gcctaccaga atctacagcc attcactgtt acctgtccta cgttcaggac atgtcaaagc
     3121 ctttgcccat attactggtg gaggattact agagaacatc cccagagtcc tccctgagaa
     3181 acttggggta gatttagatg cccagacctg gaggatcccc agggtcttct catggttgca
     3241 gcaggaagga cacctctctg aggaagagat ggccagaaca tttaactgtg gggttggcgc
     3301 tgtccttgtg gtatcaaagg agcagacaga gcagattctg agggatatcc agcagcacaa
     3361 ggaagaagcc tgggtgattg gcagtgtggt tgcacgagct gaaggttccc cacgtgtgaa
     3421 agtcaagaat ctgattgaaa gcatgcaaat aaatgggtca gtgttgaaga atggctccct
     3481 gacaaatcat ttctcttttg aaaaaaaaaa ggccagagtg gctgtcttaa tatctggaac
     3541 aggatcgaac ctgcaagcac ttatagacag tactcgggaa ccaaatagct ctgcacaaat
     3601 tgatattgtt atctccaaca aagccgcagt agctgggtta gataaagcgg aaagagctgg
     3661 tattcccact agagtaatta atcataaact gtataaaaat cgtgtagaat ttgacagtgc
     3721 aattgaccta gtccttgaag agttctccat agacatagtc tgtcttgcag gattcatgag
     3781 aattctttct ggcccctttg tccaaaagtg gaatggaaaa atgctcaata tccacccatc
     3841 cttgctccct tcttttaagg gttcaaatgc ccatgagcaa gccctggaaa ccggagtcac
     3901 agttactggg tgcactgtac actttgtagc tgaagatgtg gatgctggac agattatttt
     3961 gcaagaagct gttcccgtga agaggggtga tactgtcgca actctttctg aaagagtaaa
     4021 attagcagaa cataaaatat ttcctgcagc ccttcagctg gtggccagtg gaactgtaca
     4081 gcttggagaa aatggcaaga tctgttgggt taaagaggaa ctcgaggcaa gtactggttc
     4141 ggaattcatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     4201 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     4261 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     4321 aacacttgtc actactttca cttatggtgt tcaatgcttt tcaagatacc cagatcacat
     4381 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     4441 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     4501 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     4561 acacaaattg gaatataact ataactcaca caatgtatac atcatggcag acaaacaaaa
     4621 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     4681 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     4741 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     4801 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     4861 ataataagga tcccaggagc tcggcaagct tgcggccgca ctcgacccgg gcggccgctt
     4921 ccctttagtg agggttaatg cttcgagcag acatgataag atacattgat gagtttggac
     4981 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     5041 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     5101 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca
     5161 aatgtggtaa aatccgataa ggatcgatcc gggctggcgt aatagcgaag aggcccgcac
     5221 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc
     5281 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5341 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5401 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5461 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5521 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5581 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5641 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5701 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     5761 cacaccgcat acgcggatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac
     5821 ttggttaggt accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     5881 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5941 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6001 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6061 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6121 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6181 aggcttttgc aaaaagcttg attcttctga cacaacagtc tcgaacttaa ggctagagcc
     6241 accatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6301 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6361 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6421 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6481 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6541 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6601 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgagcacg
     6661 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6721 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6781 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6841 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6901 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6961 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7021 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgatgg
     7081 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     7141 agcgataagg atccgcgtat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7201 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7261 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7321 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7381 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7441 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7501 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7561 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7621 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7681 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7741 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7801 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7861 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7921 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7981 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8041 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8101 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8161 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8221 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8281 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8341 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8401 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8461 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8521 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8701 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atggctcgac agatct
//
