LOCUS       sequence-200734-       12064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgenesis, EGFP membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12064)
  AUTHORS   Icha J, Kunath C, Rocha-Martins M, Norden C
  TITLE     Independent modes of ganglion cell translocation ensure correct 
            lamination of the zebrafish retina.
  JOURNAL   J Cell Biol. 2016 Oct 24;215(2):259-275.
  PUBMED    27810916
REFERENCE   2  (bases 1 to 12064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-10-24"; volume: "215(2)"; pages: "259-275"
COMMENT     Sequence Label: ath5:GFP-CAAX
FEATURES             Location/Qualifiers
     source          1..12064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(778..789,790..952)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1324..1344
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1346..1364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1389..1405
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1390..1406
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1440..1456)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6393..6409)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6446..6464)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6464..6488
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(6494..6496,6497..6499,6500..7210)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6494..6496,6497..6499,6500..7210)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6539..6560)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6800..6819)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7147..7168
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7290..7314)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7319..7453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7343..7362)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7397..7416
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9594..9781,9782..9793)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9827..9843)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(9880..9898)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9919..9935)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9919..9935)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9932..9954)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9943..9959
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9967..9973,9974..9991,9992..9997))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10012..10033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10150..10167)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10321..10909)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10401..10420)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11080..11871,11872..11940))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11703..11722
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11941..12045)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caggtcattg cacaacacca gaaatgccct ctgatctgca aaagacgtga atatctgttc
      721 agacacccat atccactctg ttccacacag gtcagaggtt tgtccaggag ttcttgacag
      781 aggtgtaaaa agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt
      841 ttactcaatt aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt
      901 actccattaa aattgtactt gagtattaag gaagtaaaag taaaagcaag aaagaaaact
      961 agagattctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca tacaaggttt
     1021 tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac aatctaatgc
     1081 cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa gctgattctc
     1141 aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg cagtgctgaa
     1201 aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag agaggctgca
     1261 aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca gatgggccga
     1321 tatcaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat tgggtaccgg
     1381 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
     1441 atccactagt tctagaattt aaatatttta aaaatgtata tatataataa atgtatatat
     1501 atataaatgt ataatgagca aaattctaag ttttatgcaa ataaaaaaag tattatggaa
     1561 aatgtattat attgcagttc taaattattt aagaaaacgt ggctgacttg ccaaaatgat
     1621 ggtaaactgt gcaactctgc ttccaagatg ttagaaacag acgtttttct tttagcctca
     1681 taatttaatg ctgttgcgat gttaccgtct cttcgctgta ctggttgtta ccaggttacg
     1741 gaaaaatata acatgctcac aacatccaat cagattaaat gaactgaaaa gcaatatggg
     1801 ttttaagaca tcacagagaa ggtaacattt aaaggcttaa aagcacaaac tgtttctcgc
     1861 ttctaagact aagtaagtaa gtttttgcac acaattaaca aatagttgca ggcaaattaa
     1921 actttagtga caaggcagta ctggcccgat caggccagta atgattttgt ctactgtcca
     1981 aagcatctct cacgctggcc ctgggtcatc aagcagttct tattgttgag ccctgcatta
     2041 taattatata caattttttt ttttattatt taattttttt aaattaaaat gttatgtaat
     2101 gttatgttaa taaatgttta aataagaata ttaaaaaata atttgattta atacaaatac
     2161 agggaagcac gttttaaaaa gtaaataaat tttgttaaat tttatataag gaaaatatgt
     2221 ttatttctgt atttttctat acactgaaca caactaagaa tttctaaata aatatagaca
     2281 aatgctatta ggcatgggat gataaccagt ttaaaggtca aggtttaaaa acccccagaa
     2341 ttatctgtta taccgttcca acgatatatg taattttttt attaacactt ttaataaaaa
     2401 aggaccctca tcatcaaact gttcttaaaa taaactatat tgtgctcaaa ggtaaaaaca
     2461 gttcttgttt tttacccaga catttaaaaa aagaatttat tttagagcag taatcacaat
     2521 accgtccgtg ataccgtgat ttttttttat caaaggtaat catacagtca caatctttta
     2581 ccggcccatg ctatttatac ggcagtgtaa aataaagtgt aaattgtaac gcgttataaa
     2641 gaattgcttc ttttaaacaa tgccacaaaa attatgaaca tttttttttt gtaaaaattt
     2701 taaaccaaaa ataactaaaa atttctccaa atataaaaca attgagcagg gcacattgta
     2761 acacataacg aaaactgctt ccattgtttt tttttatact tgaaaatcct tttcatcttt
     2821 gtcccacata agtcataaca ttaacaataa taattgaata tagatttctt gtctaattga
     2881 ctgcccttaa taagacattg cttcattgac cagttaagat taaacataac aaccagaagg
     2941 gaacaaaaag gctcaaatag gcatgaaatt acaagcacct tgcttgacct aattacagtg
     3001 acgagcctca tctcaccaga tgccccaatc tgacggtggg ggttcaagtc tcctcatcta
     3061 tggaggtcac tgtttacaca atatgcctca cttttctgaa tgacctcatt ggtcaatatg
     3121 ccagttctgt gagaatcgat caaaaatagc tttaaagatt aaagatttta agttttcttc
     3181 tcactttttt aggtagatta tgtgttgaaa tgaacacaac agtttatacc catatctcag
     3241 agatcatatc gatgatgaac tttaatgaat gataagttat tatattatat attaaatcat
     3301 attatattat aatatattat attctatatt cgttttgata gatgtagtgt ttaaatgaac
     3361 agtttatact catctcaagg atcataaaga agtgtcatat catagaactg taaatgacaa
     3421 tgttatatta ttttgatgga cctttagata tattctttgg ttattatact gtatattttt
     3481 ggtagattat gtttacatca tagagcttta aatgactagt tctattctag tctatttgat
     3541 tttttttttt ttaaatgacc acaacagttt atactcaaat atcagtgatc ataatgttat
     3601 atcaacatat aaaatctata ttatgttata ttatattata ttatattata ttatattata
     3661 ttatattata ttttatttta tattagacag cacccattct cagttttaca tctattattt
     3721 gactgtcagc ttgacttaag taggagtttg atcattagtc gtcactttcc tcgagttcag
     3781 aggattacgc attcgatttt taagaccatt ttctccttcg gaatgaacgg cgctttatgc
     3841 tttaaatgaa aaccagattt actaaatcct ggattaagct gttaaactga taatagacct
     3901 tctcactttc ccctcgtcaa gaccgagtaa atctttaatc tgctcaacac tgtctcaaaa
     3961 attacaggag tagcttgtaa catatggcct tttgcatagg agtccagaca agcctatcat
     4021 gtgaacgagt gaaaaaacaa ggccgtttaa catgtctgga ccaagccctt taaacgggac
     4081 aagcagcagc tggtcaggga tgccaatctg caatcaatga aaacaaacta aaccagtgga
     4141 ttgggtttat atgcccaatt gaacagcaga attactcatc taatgcagat gagcgtatat
     4201 ggcttgatgg ccacagtcag ccgggacatg aattatgcag aagagatata gcgatttgtc
     4261 acttgtacac aaatcagatg taataatgta tgtatttagt gcagtatttt atatagaacg
     4321 ctactttacg atttcaagta cctgtaggaa aatatgttat agataattac tatactatag
     4381 aggtgataat tcataatgca taactttaaa aacattttgt ttcatagtat agcaattata
     4441 tatacagtat ataagataga ctgcatatat atatatatat atatatatta tatctgtgtg
     4501 ttattatatt ataaccttag tataacaata atagtatttt ggaaaggttt atgaatgagt
     4561 ttttttgcca acaggctttt tttggagggg gtgaaactgt gactgtctga atctgctttg
     4621 tttgattcac attgaacaga ttgagatcga cagcttgtcg aagacttctg tctgtctcaa
     4681 ttcctctgag acactcaaat caattattta gtgctgcccc acaaatgtgt tttatatggt
     4741 tttgttattg gtttactata tattattaaa tatatagaat gtaatatgta atatagagaa
     4801 atgtgctgat gatacagtag atgaatactg acactatatt aaaatattct atataaaagt
     4861 ataaaatttg aacactgacg ctatattaaa agattgcaaa tgaccgtata aatgtttaaa
     4921 atgtagaaac ttcacatgtt attttttcta aacaagcatg ctggttatta ctttataatt
     4981 atgtaacaat ataactgtac taatacagtt cagtaattaa caagtaattc aaaaatattt
     5041 ttattttaaa tgtttatatt ttatcattat tcttcaagcc actaggttat attagtttgt
     5101 cagcaaaaat gaacaaatta gcaccaacgc ttataattta aaatattaaa tcttgaaaat
     5161 attttaaaga atgttacatt taatgaaaat tattttaaaa agcataagaa aaattacaca
     5221 ctcaaattgc atttaatatg ctttccgtgt aaattactta tgtaaattta atttgatgca
     5281 cactaaaatg tctcgtctat gacctatata cataatgtat tatcatattg taactcaaat
     5341 attacatgta atattaatat taccttaaat atttatatat tacaatttaa cattatgaat
     5401 aacaaagaca actggagtat gtttgaccaa aacaaaacaa caatctttct atttgtaaaa
     5461 gttaatttta aaaaaactgt tgcgatgtgt aaaatatcca tttactttat tcagtaaaac
     5521 taatgtggat tataatagta tagcataaat atataacaat atatcaattt aatattaaca
     5581 tcaatataaa attgatgaat ctatctatga aaaaatatga aaaattcatt atttactcac
     5641 ttattactaa ttgcacaccc gtatactatt cttttttcat tgaacacaaa gaaagatatg
     5701 ttgaagtatg ctgaaaaccg gtaaccattg acttcaacag tatttatttt tccaatggca
     5761 gccagtggtt acaggtttgc agctttcaac caaatatttt cttttgtgat cagcagaaga
     5821 aagaaactca taaaagtttg gaaccactta aataaaatag tgcataaata gtctgtaaat
     5881 gttcattttt gtatgaacta tcccttcaaa ttatcgttta tattaaaaga ttccagtaat
     5941 aaatgtaaat aatgagacct acaaccctgt aaaacaaatt gcaaaatcaa actgactcta
     6001 tacaggcaat tactcctgca aacaatgttc tcatttaaga aatccaaaga tgtcacggtt
     6061 gcttaaagat gcatgcactt tttaattagg taatggtcct tgccacccgg cgctggttgt
     6121 ggccagatgg taagttatct ctggtttgta acgcctgttt tcagtgatag agcagccaaa
     6181 gttaattgga atgtcattaa agtttgagtt actgggggtt ggggctggac tgcactagtc
     6241 ctgctgttgg agatgtgcgt ttcattaata ataatggatg tttggatgga gtaataaggg
     6301 tggggctcca cctgctgcca cctgttaata ctctatataa gcatcgctca tccttatggc
     6361 agaacagaag cgattaagaa ccatccgggg gggatccact agttctagag cggccgccac
     6421 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattcaagtt tgtacaaaaa
     6481 agcaggctgg accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     6541 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     6601 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     6661 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     6721 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     6781 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     6841 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     6901 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     6961 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     7021 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     7081 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     7141 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     7201 gctgtacaag ggaggaggaa gatctaagct gaaccctcct gatgagagtg gccccggctg
     7261 catgagctgc aagtgtgtgc tctcctgaga cccagctttc ttgtacaaag ttgatgtaga
     7321 tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7381 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7441 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     7501 gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggccctcg agcatatgag
     7561 atctgtcccg atctttctct tctgtgctgt caatttagag tagtttttga ggtaatttgg
     7621 gtgcattctc tgtaaaaaca acaaataaat atttatattg tatgcaataa atgtaacaat
     7681 tacacataca ctctctgatt acagcattaa agggttagtt cacccaaaaa tgaaaagatg
     7741 tcattaatga ctcaccctca tgtcgttcca agcccgtaac acctccgttc atcttcggaa
     7801 cacagtttaa gatattttag atttagtccg agagctttct gtccctccac tgaaaatgta
     7861 tgtacggtat actgtccatg tccagaaagg taataaaaac atcaaagtag tccatgtgac
     7921 atcagtgggt taggtagaat tatttgaagc atcgaaaata cattttggtc caaaaataac
     7981 aaaacctacg actttattca gcattgtagc gtcactgcga agtcgtgaac gcggattgac
     8041 aacagacccg gaagagaata caatgccgaa taaagtcgta ggttttgtta tttttggacc
     8101 aaaatgtatt cgatgcttca aaaaattcta actaacccac tgatgtcaca tggactactt
     8161 tgatgttttt attacctttc tggacatgga cagtataccg tacatacatt ctcaatggag
     8221 gcacagaaag ctctcggact aaatctaaaa tatcttaaac tgtgttctga agatgaacgg
     8281 aggtcttacg ggcttggaac gacatgaggg cgagtcatta atgacattat tttcattttt
     8341 gggtgaacta accctttaat aaaactgaca tttgcagcca attgcacact ttataatgtt
     8401 aaatacttgt tgattttaga aatacagtag catttagtga caatcaaaca ttaattttat
     8461 ttctgattat attgacaaaa acacaggact gtaaggctga catgacaggg tgaattgatt
     8521 tcattacatt agttttgatg gtaacacttt tacaataagg ttcgttagtt aactacatta
     8581 gttaacatga actaataatg aactgcacgt atacatgtta atttcaacat ttactaatac
     8641 tttatatctt gttaacatta gttaatgcac tgtgaactaa catgaacaaa caatgaacat
     8701 ctgtatttct attaactaac gttaacaaag attaacagat acagtaacaa atgtattgct
     8761 catggttatt taatgttagt taatacatga actaatcaac ctcattgtaa agtgttaccg
     8821 ttttgacaat acaacatgtt tggaaaaaaa tttctcgctt ggttttcttg tgttttgcat
     8881 gaattcaaac acaatgagag cacggaatga taatgattat tcaaagctgc aataggcgcc
     8941 aaatttcccg tggacctaac gttaccaatt acaaatgcat tttagcattt tattacgtga
     9001 gccttatatt gtcaatcgca tgcattagtg aaaataggcc tactacacat ataaaaggcg
     9061 cgccatcaac gtcaaaacat gcacacccaa aaaaaaaaaa aaaaaaaaaa aaaaaaacta
     9121 tcagtaaaac aaaatactta atgtacttac ctcaatatgc ttccttaggt ttgatggcga
     9181 acttttgaag gccgatattt ctttattaag cgggagacag aggacacatt tcatcttgaa
     9241 tgaatcttta tttacaccac ttaaagaaaa gaattcgcga agatacggcc acgggtgctc
     9301 ttgatcctgt ggctgatttt ggactgtgct gctcgcagct gctgatgaat cacatacttc
     9361 ctccattttc ttccactgat tgactgttat aatttcccta atttccaggt caaggtgctg
     9421 tgcattgtgg taatagatgt gacatgacgt cacttccaaa ggaccaatga acatgtctga
     9481 ccaatttcat ataatgtgaa aacgattttc ataggcagaa taaataacat ttaaattaaa
     9541 ctgggcatca gcgcaattca attggtttgg taatagcaag ggaaaataga atgaagtgat
     9601 ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt
     9661 ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt
     9721 aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta
     9781 ctttacacct ctggttcttg accccctacc ttcagcaagc ccagcagatc cactagttct
     9841 agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     9901 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9961 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10021 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10081 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10141 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10201 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10261 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10321 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10381 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10441 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10501 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10561 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10621 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10681 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10741 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    10801 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10861 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11281 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    11521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11581 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11641 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11701 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11761 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11821 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11881 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11941 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12001 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12061 agtg
//
