LOCUS       sequence-200772-        1218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR21; Opto-GPR21; A11).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR21
FEATURES             Location/Qualifiers
     source          1..1218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtctttc actgtgcacc tttgttgaac
      301 catcacactc tcaactacat cctgctcaac ctggccgtgg ccgacctctt catggtgttc
      361 gggggcttca ccaccaccct ctacacctct ctgcacgggt acttcgtctt tgggcccacg
      421 ggctgcaacc tggagggctt ctttgccacc ttgggcggtg aaattgcact gtggtccttg
      481 gtggtcctgg ccatcgagcg gtacgtggtg gtgactaaac ctttaaccta taatactctg
      541 gttacaccct ggagagccat catgggcgtc gccttcacct gggtcatggc tctggcctgt
      601 gccgcgcccc ccctcgtcgg ctggtccagg tacatcccgg agggcatgca gtgctcgtgc
      661 gggattgact actacacgcc ccacgaggaa accaacaatg agtcgttcgt catctacatg
      721 ttcgtggtcc acttcatcat ccccctgatt gtcatattct tctgctacgg aaacatcttc
      781 cgcatctgcc aacagcacac aaaggacatc agcgaaaggc aagcccgctt cagcagccag
      841 agtggggaga ctggggaagt gcaggcctgt cctgataagc gctatgcccg catggtgatc
      901 atcatggtca tcgctttcct aatctgctgg ctgccctacg ctggggtggc gttctacatc
      961 ttcacccatc agggctctga ctttggcccc atcttcatga ccatcccggc tttctttgcc
     1021 aagacttctg ccgtctacaa ccccgtcatc tacatcatga tgaacaagca gttccgcaga
     1081 ggactaaagc gcctctcagg ggctatgtgt acttcttgtg caagtcagac tacagccaac
     1141 gacccttaca cagttagaag caaaggccct cttaatggat gtcatatcac agaaaccagc
     1201 caagtggcgc ctgcctaa
//
