LOCUS       sequence-200776-        1305 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR27; Opto-GPR27; B3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR27
FEATURES             Location/Qualifiers
     source          1..1305
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcatcg tgcgggagcg cagcctgcac
      301 cgcgccctca actacatcct gctcaacctg gccgtggccg acctcttcat ggtgttcggg
      361 ggcttcacca ccaccctcta cacctctctg cacgggtact tcgtctttgg gcccacgggc
      421 tgcaacctgg agggcttctt tgccaccttg ggcggtgaaa ttgcactgtg gtccttggtg
      481 gtcctggcca tcgagcggta cgtggtggtg gcgcaccacc gcttctatgc agagcgcctg
      541 gccggctggc cgtgcgccgc catcatgggc gtcgccttca cctgggtcat ggctctggcc
      601 tgtgccgcgc cccccctcgt cggctggtcc aggtacatcc cggagggcat gcagtgctcg
      661 tgcgggattg actactacac gccccacgag gaaaccaaca atgagtcgtt cgtcatctac
      721 atgttcgtgg tccacttcat catccccctg attgtcatat tcttctgcta cggacacgac
      781 cgccgcaaga tgcggcccgc gcgcctggtg cccgccgtca gccacgactg gaccttccac
      841 ggcccgggcg ccaccggcca ggccgccgcc aactggacgg cgggcttcgg ccgcgggccc
      901 acgccgcccg cgcttgtggg catccggccc gcagggccgg gccgcggcgc gcgccgcctc
      961 ctcgtgctgg aagaattcaa gacggagaag aggctgtgcc gcatggtgat catcatggtc
     1021 atcgctttcc taatctgctg gctgccctac gctggggtgg cgttctacat cttcacccat
     1081 cagggctctg actttggccc catcttcatg accatcccgg ctttctttgc caagacttct
     1141 gccgtctaca accccgtcat ctacatcatg atgaacaagc agttccgcga ctgcttcagg
     1201 gcccagttcc cctgctgcca gagcccccgg accacccagg cgacccatcc ctgcgacctg
     1261 aaaggcattg gtttaacaga aaccagccaa gtggcgcctg cctaa
//
